Goat Anti-Rat Secondary Antibody, Biotin Conjugated

Description
Goat anti-rat IgG recognizes rat IgG whole molecule. This secondary antibody was purified using antigen affinity chromatography. The antibody is conjugated with Biotin.

Antibody Type
Polyclonal Antibody

Purification
Liquid; This product was prepared from monospecific antiserum by immunoaffinity chromatography, followed by solid phase adsorption(s) to remove any unwanted reactivities. 

Immunogen
Rat IgG whole molecule

Isotype
IgG

Size
350 µg

Formulation
In 10 mM sodium phosphate, 75 mM NaCl, 50% (v/v) glycerol, pH 7.2 (0.01% (w/v) sodium azide, 10 mg/mL BSA (Immunoglobulin and Protease free))

Specificity
Rat

Storage
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.

Handling Recommendations
The optimal working dilution should be determined by the end user. For maximum recovery of the products, centrifuge the vial prior to opening the cap. 

Applications & Suggested Dilutions
Western Blot: 1:1000-1: 10000;  Immunohistochemistry:1:100-1:500; Immunofluorescence: 1:100-1:500; ELISA: 1:2000-1:20000.