Goat anti-rat IgG recognizes rat IgG whole molecule. This secondary antibody was purified using antigen affinity chromatography. The antibody is conjugated with Biotin.
Liquid; This product was prepared from monospecific antiserum by immunoaffinity chromatography, followed by solid phase adsorption(s) to remove any unwanted reactivities.
Rat IgG whole molecule
In 10 mM sodium phosphate, 75 mM NaCl, 50% (v/v) glycerol, pH 7.2 (0.01% (w/v) sodium azide, 10 mg/mL BSA (Immunoglobulin and Protease free))
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
The optimal working dilution should be determined by the end user. For maximum recovery of the products, centrifuge the vial prior to opening the cap.
Applications & Suggested Dilutions
Western Blot: 1:1000-1: 10000; Immunohistochemistry:1:100-1:500; Immunofluorescence: 1:100-1:500; ELISA: 1:2000-1:20000.