DescriptionGoat anti-rabbit IgG recognizes rabbit IgG whole molecule. This secondary antibody was purified using antigen affinity chromatography. The antibody is conjugated with biotin.Antibody TypePolyclonal AntibodyPurificationLiquid; This product was prepared from monospecific antiserum by immunoaffinity chromatography, followed by solid phase adsorption(s) to remove any unwanted reactivities. ImmunogenRabbit IgG whole moleculeIsotypeIgG Size350 µg
FormulationIn 10 mM sodium phosphate, 75 mM NaCl, 50% (v/v) glycerol, pH 7.2 (0.01% (w/v) sodium azide, 10 mg/mL BSA (Immunoglobulin and Protease free))SpecificityRabbitStorageStore at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.Handling RecommendationsThe optimal working dilution should be determined by the end user. For maximum recovery of the products, centrifuge the vial prior to opening the cap. Applications & Suggested DilutionsWestern Blot: 1:1000-1: 10000; Immunohistochemistry:1:100-1:500; Immunofluorescence: 1:100-1:500; ELISA: 1:2000-1:20000
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