Background
Histone H3 along with H2A, H2B and H4 is involved in the structure of chromatin in eukaryotic cells. Histone H3 can undergo several different types of epigenetic modifications that influence cellular processes. These modifications, including acetylation, phosphorylation, methylation, ubiquitination, and ADP-ribosylation, occur on the N-terminal tail domains of histone H3, which results in remodeling of the nucleosome structure into an open conformation more accessible to transcription complexes. In most species, histone H3 is primarily acetylated at lysine 9, 14, 18, 23, and can also be acetylated at lysine 56.

Description
Histone H3K56ac (Acetyl H3K56) Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Liquid. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3 .

Specificity
Mouse, Rat, Human, Broad Range

Isotype
IgG

Uniprot ID
Q16695

Purification
Affinity Purified

Immunogen
Synthetic Peptide of Human Acetyl-Histone H3-K56

Storage
Shipped at 4°C. Store at -20°C. Avoid multiple freeze/thaw cycles.

Alternative Names
H3K56ac antibody, H3K56a antibody

Application
WB, IHC, IF, IP, ChIP, ChIPseq

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von Walden F et. al. (August 2016). mTOR signaling regulates myotube hypertrophy by modulating protein synthesis, rDNA transcription and chromatin remodeling. Am J Physiol Cell Physiol. :ajpcell.00144.2016.

Pal S et. al. (May 2016). The Commercial Antibodies Widely Used to Measure H3 K56 Acetylation Are Non-Specific in Human and Drosophila Cells. PLoS One. 11(5):e0155409.

Zhang ZQ et. al. (April 2016). Epigenetic regulation of NKG2D ligands is involved in exacerbated atherosclerosis development in Sirt6 heterozygous mice. Sci Rep. 6:23912.

Elhanati S et. al. (September 2013). Multiple regulatory layers of SREBP1/2 by SIRT6. Cell Rep. 4(5):905-12.