We recommend our top selling Histone H3 Modification Multiplex Assay Kit to quantify 21 different histone H3 modifications.
BackgroundHistone H3- along with H2A, H2B, and H4- is involved in the structure of chromatin in eukaryotic cells. Histone H3 can undergo several different types of epigenetic modifications that influence cellular processes. These modifications, including acetylation, phosphorylation, methylation, ubiquitination, and ADP-ribosylation, occur on the N-terminal tail domains of histone H3, which results in the remodeling of the nucleosome structure into an open conformation more accessible to transcription complexes. In most species, histone H3 is primarily acetylated at lysine 9, 14, 18, and 23.
DescriptionHistone H3K9ac (Acetyl H3K9) Polyclonal Antibody. Unconjugated. Raised in: Rabbit.
Formulation:Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3
SpecificityBroad Range, Human, Mouse, Rat
IsotypeIgG
Uniprot IDQ16695
PurificationAffinity Purified
ImmunogenA synthetic acetylated peptide around K9 of human Histone H3 (NP_003520.1).
StorageShipped at 4°C. Store at -20°C. Avoid multiple freeze/thaw cycles.
Alternative NamesH3K9ac antibody, H3K9a antibody
ApplicationWB, IHC, IF, IP, ChIP, ChIPseq; Recommended dilution: WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:50 - 1:200, IP 1:50 - 1:200, ChIP 1:20 - 1:100, CHIPseq 1:20 - 1:50