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Histone H3K36me3 (H3K36 Trimethyl) Polyclonal Antibody

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Citations (4) | Write a Review
Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using Histone H3K36me3 Polyclonal Antibody.
Dot-blot analysis of all sorts of methylation peptides using Histone H3K36me3 Polyclonal Antibody.
Immunohistochemistry of paraffin-embedded rat lung tissue using Histone H3K36me3 Polyclonal Antibody at dilution of 1:200 (x400 lens).
Immunohistochemistry of paraffin-embedded rat spleen tissue using Histone H3K36me3 Polyclonal Antibody at dilution of 1:200 (x400 lens).
Immunohistochemistry of paraffin-embedded human lung cancer tissue using Histone H3K36me3 Polyclonal Antibody at dilution of 1:200 (x400 lens).
Immunohistochemistry of paraffin-embedded human rectal tissue using Histone H3K36me3 Polyclonal Antibody at dilution of 1:200 (x400 lens).
Immunohistochemistry of paraffin-embedded mouse brain tissue using Histone H3K36me3 Polyclonal Antibody at dilution of 1:200 (x400 lens).
Immunofluorescence analysis of 293T cell using Histone H3K36me3 Polyclonal Antibody. Blue: DAPI for nuclear staining.
Chromatin immunoprecipitation analysis of γ-actin gene from 293 cell line, using Histone H3K36me3 Polyclonal Antibody and rabbit IgG. P1, P2 and P3 were probes located on γ-actin gene as the schematic diagram illustrated. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Application: ChIP, ChIPseq, IF, IHC, IP, WB
Areas of Research: Histone Methylation & Demethylation
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Affinity Purified
Reactivity: Broad Range, Human, Mouse, Rat
100% Guarantee: 6 months
Catalog No.SizePriceQty
A-4042-02525 µl $147.00 
A-4042-05050 µl $285.00 
Availability: Usually Ships In 1-2 Days 
Product Overview

Description
Histone H3K36me3 (H3K36 Trimethyl) Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Specificity
Broad Range, Mouse, Rat, Human

Isotype
IgG

Uniprot ID
Q16695

Purification
Affinity Purified

Immunogen
Synthetic Peptide of Human TriMethyl-Histone H3-K36

Storage
Shipped at 4°C. Store at -20°C. Avoid multiple freeze/thaw cycles.

Application
WB, IHC, IF, IP, ChIP, ChIPseq

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

Product Citations

Onyango DO et. al. (April 2016). Tetratricopeptide repeat factor XAB2 mediates the end resection step of homologous recombination. Nucleic Acids Res.

Creamer KM et. al. (June 2014). The Mi-2 Homolog Mit1 Actively Positions Nucleosomes within Heterochromatin To Suppress Transcription. Mol Cell Biol. 34(11):2046-61.

Schroeder EA et. al. (June 2013). Epigenetic silencing mediates mitochondria stress-induced longevity. Cell Metab. 17(6):954-64.

Ma P et. al. (February 2012). Compensatory functions of histone deacetylase 1 (HDAC1) and HDAC2 regulate transcription and apoptosis during mouse oocyte development. Proc Natl Acad Sci U S A. 109(8):E481-9.

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