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Histone H3K27me3 (H3K27 Trimethyl) Polyclonal Antibody

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Western blot analysis of various lysates using Histone H3K27me3 (H3K27 Trimethyl) Polyclonal Antibody at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 0.5s
Dot-blot analysis of all sorts of methylation peptides using Histone H3K27me3 (H3K27 Trimethyl) Polyclonal Antibody.
Immunohistochemistry analysis of paraffin-embedded Human testis using Histone H3K27me3 (H3K27 Trimethyl) Polyclonal Antibody at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of PC-12 cells using Histone H3K27me3 (H3K27 Trimethyl) Polyclonal Antibody at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Histone H3K27me3 (H3K27 Trimethyl) Polyclonal Antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Histone H3K27me3 (H3K27 Trimethyl) Polyclonal Antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Application: ChIP, ChIPseq, ELISA, IF/ICC, IHC, IP, WB
Areas of Research: Histone Methylation & Demethylation
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Affinity Purified
Reactivity: Broad Range, Human, Mouse, Rat
100% Guarantee: 6 months
Catalog No.SizePriceQty
A-4039-02525 µl $180.00 
A-4039-05050 µl $350.00 
A-4039-100100 µl $486.00 
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Product Overview

Description
Histone H3K27me3 (H3K27 Trimethyl) Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.

Specificity
Broad Range, Mouse, Rat, Human

Isotype
IgG

Uniprot ID
Q16695/P68431

Purification
Affinity Purified

Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human histone H3 (NP_003520.1).

Storage
Shipped at 4°C. Upon receipt, store at -20°C. Avoid repeated freeze.

Alternative Names
HIST3H3, H3/g

Application
WB, IHC, IF/ICC, IP, ChIP, ChIPseq, ELISA; Recommended dilution: WB 1:500 - 1:1000, IHC 1:50 - 1:200, IF/ICC 1:50 - 1:200, IP 0.5ug-4ug antibody for 400ug-600ug extracts of whole cells, ChIP 5μg antibody for 5μg-10μg of Chromatin, CHIPseq 1:20 - 1:100, ELISA - recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Datasheet & SDS

Datasheet/User Guide
If the above file is not available online, you can request a copy by contacting us.

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