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Histone H3K27me3 (H3K27 Trimethyl) Polyclonal Antibody

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Western Blot - Histone H3K27me3 (H3K27 Trimethyl) Polyclonal Antibody
Immunohistochemistry - Histone H3K27me3 (H3K27 Trimethyl) Polyclonal Antibody
Immunohistochemistry - Histone H3K27me3 (H3K27 Trimethyl) Polyclonal Antibody
Immunohistochemistry - Histone H3K27me3 (H3K27 Trimethyl) Polyclonal Antibody
Immunohistochemistry - Histone H3K27me3 (H3K27 Trimethyl) Polyclonal Antibody
Immunohistochemistry - Histone H3K27me3 (H3K27 Trimethyl) Polyclonal Antibody
Immunofluorescence - Histone H3K27me3 (H3K27 Trimethyl) Polyclonal Antibody
Immunofluorescence - Histone H3K27me3 (H3K27 Trimethyl) Polyclonal Antibody
Immunofluorescence - Histone H3K27me3 (H3K27 Trimethyl) Polyclonal Antibody
Immunofluorescence - Histone H3K27me3 (H3K27 Trimethyl) Polyclonal Antibody
Dot Blot - Histone H3K27me3 (H3K27 Trimethyl) Polyclonal Antibody
Chromatin Immunoprecipitation - Histone H3K27me3 (H3K27 Trimethyl) Polyclonal Antibody
Application: ChIP, ChIPseq, IF, IHC, IP, WB
Areas of Research: Histone Methylation & Demethylation
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Affinity Purified
Reactivity: Broad Range, Human, Mouse, Rat
100% Guarantee: 6 months
Catalog No.SizePriceQty
A-4039-02525 µl $147.00 
A-4039-05050 µl $285.00 
Availability: Usually Ships in 3-4 Days 
Product Overview

Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replicationdependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

Description
Histone H3K27me3 (H3K27 Trimethyl) Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Specificity
Broad Range, Mouse, Rat, Human

Isotype
IgG

Uniprot ID
Q16695

Purification
Affinity Purified

Immunogen
Synthetic Peptide of Human TriMethyl-Histone H3-K27

Storage
Shipped at 4°C. Store at -20°C. Avoid multiple freeze/thaw cycles.
 

Application
WB, IHC, IF, IP, ChIP, ChIPseq; Recommended dilution: WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200 ChIP 1:20 - 1:100 CHIPseq 1:20 - 1:100

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

Product Citations

Colón-Caraballo M et. al. (February 2018). Effects of histone methyltransferase inhibition in endometriosis. Biol Reprod.

Warren Vieira et. al. (December 2017). Trimethylation of Histone 3 lysine 27 (H3K27me3) ChIP-PCR and transcriptional expression data of Ef1-alpha, cyp26A, HoxC10, HoxD10 and HoxD11 in the Xenopus XTC cell line Data in Brief. 15:970-974.

Cascio S et. al. (December 2017). Abnormally glycosylated MUC1 establishes a positive feedback circuit of inflammatory cytokines, mediated by NF-κB p65 and EzH2, in colitis-associated cancer. Oncotarget. 8(62):105284-105298.

Xiong Y et. al. (August 2017). Effects of high progesterone level on the day of human chorionic gonadotrophin administration in in vitro fertilization cycles on epigenetic modification of endometrium in the peri-implantation period. Fertil Steril. 108(2):269-276.e1.

Upadhyaya B et. al. (April 2017). Prenatal Exposure to a Maternal High-Fat Diet Affects Histone Modification of Cardiometabolic Genes in Newborn Rats. Nutrients. 9(4)

Yadav R et. al. (February 2017). EZH2 regulates spinal neuroinflammation in rats with neuropathic pain. Neuroscience.

Timoshevskiy VA et. al. (June 2016). Cellular and Molecular Features of Developmentally Programmed Genome Rearrangement in a Vertebrate (Sea Lamprey: Petromyzon marinus). PLoS Genet. 12(6):e1006103.

Eid A et. al. (February 2016). Developmental lead exposure and lifespan alterations in epigenetic regulators and their correspondence to biomarkers of Alzheimer's disease Alzheimer's and Dement. :1-9.

Barroso M et. al. (October 2015). S-adenosylhomocysteine induces inflammation through NFkB: A possible role for EZH2 in endothelial cell activation. Biochim Biophys Acta. 1862(1):82-92.

Pacini L et. al. (September 2015). Down-regulation of toll-like receptor 9 expression by beta human papillomavirus type 38 and implications for cell cycle control. J Virol.

Guo X et. al. (December 2014). Regulation of histone demethylase KDM6B by hypoxia-inducible factor-2α Acta Biochim Biophys Sin (Shanghai).

Siouda M et. al. (May 2014). Epstein-Barr virus down-regulates tumor suppressor DOK1 expression. PLoS Pathog. 10(5):e1004125.

Guo X et. al. (March 2014). Nicotine induces alteration of H3K27 demethylase UTX in kidney cancer cell. Hum Exp Toxicol. 33(3):264-9.

Saidj D et. al. (November 2013). Oncoprotein E7 from beta human papillomavirus 38 induces formation of an inhibitory complex for a subset of p53-regulated promoters. J Virol. 87(22):12139-50.

Accardi R et. al. (March 2013). Epstein - Barr virus transforming protein LMP-1 alters B cells gene expression by promoting accumulation of the oncoprotein ΔNp73α. PLoS Pathog. 9(3):e1003186.

Siouda M et. al. (December 2012). Transcriptional regulation of the human tumor suppressor DOK1 by E2F1. Mol Cell Biol. 32(23):4877-90.

Shen Y et. al. (October 2012). Expression and significance of histone H3K27 demethylases in renal cell carcinoma. BMC Cancer. 12:470.

Ding YB et. al. (September 2012). 5-aza-2'-deoxycytidine leads to reduced embryo implantation and reduced expression of DNA methyltransferases and essential endometrial genes. PLoS One. 7(9):e45364.

Ramming A et. al. (May 2012). Maturation-related histone modifications in the PU.1 promoter regulate Th9-cell development. Blood. 119(20):4665-74.

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