We recommend our top selling Histone H3 Modification Multiplex Assay Kit to quantify 21 different histone H3 modifications.
Background Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replicationdependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Description Histone H3K27me3 (H3K27 Trimethyl) Polyclonal Antibody. Unconjugated. Raised in: Rabbit.
Formulation PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
SpecificityBroad Range, Mouse, Rat, Human
IsotypeIgG
Uniprot ID Q16695
PurificationAffinity Purified
ImmunogenSynthetic Peptide of Human TriMethyl-Histone H3-K27
StorageShipped at 4°C. Store at -20°C. Avoid multiple freeze/thaw cycles.
ApplicationWB, IHC, IF, IP, ChIP, ChIPseq; Recommended dilution: WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200 ChIP 1:20 - 1:100 CHIPseq 1:20 - 1:100
