Background Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replicationdependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Description Histone H3K27me3 (H3K27 Trimethyl) Polyclonal Antibody. Unconjugated. Raised in: Rabbit.
Formulation PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
SpecificityBroad Range, Mouse, Rat, Human
IsotypeIgG
Uniprot ID Q16695
PurificationAffinity Purified
ImmunogenSynthetic Peptide of Human TriMethyl-Histone H3-K27
StorageShipped at 4°C. Store at -20°C. Avoid multiple freeze/thaw cycles.
ApplicationWB, IHC, IF, IP, ChIP, ChIPseq; Recommended dilution: WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200 ChIP 1:20 - 1:100 CHIPseq 1:20 - 1:100
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