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Histone H3K27me3 (H3K27 Trimethyl) Polyclonal Antibody

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Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K27 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K27 antibody.
Immunohistochemistry of paraffin-embedded rat heart using TriMethyl-Histone H3-K27 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using TriMethyl-Histone H3-K27 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using TriMethyl-Histone H3-K27 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat ovary using TriMethyl-Histone H3-K27 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using TriMethyl-Histone H3-K27 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using TriMethyl-Histone H3-K27 antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using TriMethyl-Histone H3-K27 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using TriMethyl-Histone H3-K27 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using TriMethyl-Histone H3-K27 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Chromatin immunoprecipitation analysis of extracts of 293T cells, using TriMethyl-Histone H3-K27 antibody and rabbit IgG. P1 and P2 were located on ANO2 gene. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Application: ChIP, ChIPseq, IF, IHC, IP, WB
Areas of Research: Histone Methylation & Demethylation
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Affinity Purified
Reactivity: Broad Range, Human, Mouse, Rat
100% Guarantee: 6 months
Catalog No.SizePriceQty
A-4039-02525 µl $161.00 
A-4039-05050 µl $311.00 
A-4039-100100 µl $436.00 
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Product Overview

Description
Histone H3K27me3 (H3K27 Trimethyl) Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Specificity
Broad Range, Mouse, Rat, Human

Isotype
IgG

Uniprot ID
Q16695

Purification
Affinity Purified

Immunogen
A synthetic trimethylated peptide around K27 of human histone H3 (NP_003520.1)

Storage
Shipped at 4°C. Upon receipt, store at -20°C. Avoid repeated freeze.

Alternative Names
HIST3H3, H3/g

Application
WB, IHC, IF, IP, ChIP, ChIPseq; Recommended dilution: WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200 ChIP 1:20 - 1:100 CHIPseq 1:20 - 1:100

Datasheet & SDS

Datasheet/User Guide
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