EpiQuik In Situ DNA Damage H2AX-Ser139 (γH2AX) Assay Kit

The EpiQuik™ In Situ DNA Damage H2AX-Ser139 (γH2AX, or gamma H2AX) Assay Kit is a convenient set of tools that allows the experimenter to detect DNA damage or apoptosis by measuring phosphorylation of H2AX^Ser139 in situ. The kit is ready-to-use and provides all the essential components needed for specifically measuring DNA damage in situ through phospho H2AX^Ser139 detection using cultured adherent cells. The kit has the following advantages:

• Quick and efficient procedure, which can be finished within 3 hours.
• Innovative colorimetric assay without the need for radioactivity, electrophoresis, and chromatography.
• Measurement of in situ histone H2AX phosphorylation without the need to prepare cell lysates.
• Microplate format makes the assay suitable for high throughput analysis of agents that increases or inhibits DNA damage.
• Simple, reliable, and consistent assay conditions.

Principle & Procedure
EpiQuik™ In Situ DNA Damage H2AX-Ser139 (γH2AX) Assay Kit is a whole cell-based detection of DNA damage and/or apoptosis. In this assay, adherent cells are cultured in conventional 96 -well microplates. After your experimental treatment, cells are fixed and permeabilized. The phosphorylation of H2AX at serine139, the sensitive marker of DNA damage, is then detected by an anti-phospho H2AX^Ser139 antibody. The ratio or amount of phospho H2AX^Ser139 can be quantified through HRP conjugated secondary antibody-color development system and is proportional to the intensity of color development.

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