Superoxide dismutase (SOD) is one of the most important enzymes in defending oxidative stress. SOD catalyses the dismutation of superoxide anion (O2-) into hydrogen peroxide and molecular oxygen (O2). Oxidative stress through reactive oxygen species (ROS) plays an important role in the etiology and progression in certain diseases such as cancer, heart disease, and various autoimmune disorders. ROS have shown to cause cellular damage, enzyme inactivation, and more particularly, epigenetic damage of DNA. Thus, the determination of SOD activity is necessary in various research fields related to human diseases. There are several methods used for measuring SOD activity, yet most of these methods have some drawbacks in selectivity, rapidity, and convenience. The EpiQuik Superoxide Dismutase Activity/Inhibition Assay Kit (Colorimetric) addresses these problems by using a unique procedure to measure SOD activity. The kit has the following advantages:
- The fastest procedure available, which can be finished within 60 minutes.
- Very simple -- just one step.
- High throughput microplate format with consistent reaction conditions.
Principle & Procedure
The EpiQuik™ Superoxide Dismutase Activity/Inhibition Assay Kit (Colorimetric) utilizes a dye that produces a water-soluble formazan upon reduction with superoxide anion. The rate of the reduction with a superoxide anion is linearly related to the xanthine oxidase (XO) activity, and is inhibited by SOD. Therefore, the inhibition activity of SOD can be determined by measuring superoxide anion-caused formation of water-soluble dye (color intensity). The higher the SOD activity, the less the formation of formazan and the lower the OD value.