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FitAmp Blood and Cultured Cell DNA Extraction Kit

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The FitAmp™ Blood and Cultured Cell DNA Extraction kit simply applies our proprietary DNA isolation buffer to cell pellets. After treatment with DNA digestion buffer, the DNA is easily recovered in 10-20 µl by our specially designed F-Spin Column. DNA is ready for downstream applications.
Genomic DNA was isolated from MCF-7 cell line using the FitAmp™ Blood and Cultured Cell DNA Extraction kit. The isolated DNA yield was quantified by real time PCR.
Input Type: Unisolated Samples
Research Area: DNA Damage & Repair, DNA Methylation, Next Gen Sequencing
Target Application: Sample Isolation
Vessel Format: Columns/Tubes
100% Guarantee: 6 months
Catalog No.SizePriceQty
P-1018-05050 samples $125.00 
P-1018-100100 samples $222.00 
Availability: Usually Ships In 1-2 Days 
Product Overview

The FitAmp™ Blood and Cultured Cell DNA Extraction Kit is a complete set of essential components which enables the experimenter to efficiently isolate DNA from blood leukocytes and cultured mammalian cells. The entire procedure can be completed within 20 minutes. 

WHY CHOOSE THE FITAMP™ BLOOD AND CULTURED CELL DNA EXTRACTION KIT,

  • Fast procedure which can be finished within 20 minutes with consistent isolation conditions.
  • High efficiency of DNA isolation from blood leukocytes or cultured mammalian cells.
  • Use of non-toxic reagents and no phenol chloroform.


Fig. 1.  The FitAmp Blood and Cultured Cell DNA Extraction kit simply applies our proprietary DNA isolation buffer to cell pellets. After treatment with DNA digestion buffer, the DNA is easily recovered in 10-20 µl by our specially designed F-Spin Column. DNA is ready for downstream applications.

 
Fig. 2.  Genomic DNA was isolated from MCF-7 cell line using the FitAmp Blood and Cultured Cell DNA Extraction kit. The isolated DNA yield was quantified by real time PCR.

Product Components

CB1 (Suspending buffer)
CB2 (DNA digestion solution)
CB3 (DNA digestion powder)
CB4 (DNA capture buffer)
CB5 (DNA elution solution)
F-spin column
F-collection tube
User guide

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Material Safety Data Sheet]
Product Citations

Liu X et. al. (October 2017). Effects of 2-amino-9H-pyrido[2,3-b]indole (AαC) metabolic bio-activation on oxidative DNA damage in human hepatoma G2 (HepG2) cells. Toxicol Mech Methods. :1-29.

Trivedi M et. al. (June 2017). MicroRNA-34a Encapsulated in Hyaluronic Acid Nanoparticles Induces Epigenetic Changes with Altered Mitochondrial Bioenergetics and Apoptosis in Non-Small-Cell Lung Cancer Cells. Sci Rep. 7(1):3636.

Liu Z et. al. (June 2016). DNA Demethylation Rescues the Impaired Osteogenic Differentiation Ability of Human Periodontal Ligament Stem Cells in High Glucose. Sci Rep. 6:27447.

Chen S et. al. (March 2016). High-Sensitivity and High-Efficiency Detection of DNA Hydroxymethylation in Genomic DNA by Multiplexing Electrochemical Biosensing. Anal Chem.

Morfouace M et. al. (October 2015). Preclinical studies of 5-fluoro-2'-deoxycytidine and tetrahydrouridine in pediatric brain tumors. J Neurooncol.

Zhang TT et. al. (March 2015). Genotoxic and oxidative stress effects of 2-amino-9H-pyrido[2,3-b]indole in human hepatoma G2 (HepG2) and human lung alveolar epithelial (A549) cells. Toxicol Mech Methods. :1-11.

Jung SH et. al. (October 2014). Molecular mechanisms of repeated social defeat-induced glucocorticoid resistance: Role of microRNA. Brain Behav Immun.

Trivedi M et. al. (May 2014). Morphine induces redox-based changes in global DNA methylation and retrotransposon transcription by inhibition of excitatory amino acid transporter type 3-mediated cysteine uptake. Mol Pharmacol. 85(5):747-57.

Ghosh SK et. al. (July 2013). Comparison of epigenetic profiles of human oral epithelial cells from HIV-positive (on HAART) and HIV-negative subjects. Epigenetics. 8(7):703-9.

Li W et. al. (June 2011). Distribution of 5-hydroxymethylcytosine in different human tissues. J Nucleic Acids. 2011:870726.

R. Ortiz et. al. Global DNA Methylation in Peripheral Blood mononuclear cells predicts prolonged elevations of plasma Il-6 in Women throughout the Breast Cancer Trajectory. . Abstract

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