EpiQuik Histone Methyltransferase Activity/Inhibition Assay Kit (H3K9)

HK1 (10X Wash Buffer)
HK2 (Histone Assay Buffer)
HK3 (Adomet)*
HK4 (Biotinylated Substrate, 25 µg/ml)*
HK5 (HMT Standard, 10 µg/ml)*
HK6 (Capture Antibody, 100 µg/ml)*
HK7 (Detection Antibody, 200 µg/ml)*
HK8 (Developing Solution)
HK9 (Stop Solution)
Control Enzyme (150 µg/ml)*
8-Well Assay Strips (with frame)
User Guide

* For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap.

1. Can the nuclear extract be increased for the reaction?
Yes, but not more than 6 µl.

3. If the protein concentration extracted is low (0.5 µg/µl), how much extraction can be added in the reaction?
Increase the concentration of nuclear protein during protein extraction (using more sample amount or reduce volume), and then add more sample solution to the reaction (up to 6 µl).

Xue Y et. al. (July 2017). RIZ1 and histone methylation status in pituitary adenomas. Tumour Biol. 39(7):1010428317711794.

Guan M et. al. (June 2017). Fatty acid synthase reprograms the epigenome in uterine leiomyosarcomas. PLoS One. 12(6):e0179692.

Kenji Ishimoto et. al. (October 2016). Ubiquitination of Lysine 867 of the Human SETDB1 Protein Upregulates Its Histone H3 Lysine 9 (H3K9) Methyltransferase Activity. PLOS One.

Navakauskienė et. al. (May 2016). Histone demethylating agents as potential S-adenosyl-L-methionine-competitors Med Chem Comm.

Liu J et. al. (January 2016). Clemastine Enhances Myelination in the Prefrontal Cortex and Rescues Behavioral Changes in Socially Isolated Mice. J Neurosci. 36(3):957-62.

Nizialek EA et. al. (December 2015). Cancer-predisposition gene KLLN maintains pericentric H3K9 trimethylation protecting genomic stability. Nucleic Acids Res.

Veazey KJ et. al. (September 2015). Dose-dependent alcohol-induced alterations in chromatin structure persist beyond the window of exposure and correlate with fetal alcohol syndrome birth defects. Epigenetics Chromatin. 8:39.

Cheedipudi S et. al. (July 2015). A fine balance: epigenetic control of cellular quiescence by the tumor suppressor PRDM2/RIZ at a bivalent domain in the cyclin a gene. Nucleic Acids Res. 43(13):6236-56.

Liu J et. al. (January 2015). Chromatin landscape defined by repressive histone methylation during oligodendrocyte differentiation. J Neurosci. 35(1):352-65.

Austermann J et. al. (December 2014). Alarmins MRP8 and MRP14 Induce Stress Tolerance in Phagocytes under Sterile Inflammatory Conditions. Cell Rep. 9(6):2112-23.

Morishita M et. al. (December 2014). In vitro histone lysine methylation by NSD1, NSD2/MMSET/WHSC1, and NSD3/WHSC1L. BMC Struct Biol. 14(1):25.

Sahdeo S et. al. (August 2014). Dyclonine rescues frataxin deficiency in animal models and buccal cells of patients with Friedreich's ataxia. Hum Mol Genet.

Li B et. al. (July 2013). Identification and characterization of the Spodoptera Su(var) 3-9 histone H3K9 trimethyltransferase and its effect in AcMNPV infection. PLoS One. 8(7):e69442.

Han YC et. al. (June 2013). Metallothionein 1 h tumour suppressor activity in prostate cancer is mediated by euchromatin methyltransferase 1. J Pathol. 230(2):184-93.

Horrillo A et. al. (April 2013). Zebularine regulates early stages of mESC differentiation: effect on cardiac commitment. Cell Death Dis. 4:e570.

Dong C et. al. (March 2013). Interaction with Suv39H1 is critical for Snail-mediated E-cadherin repression in breast cancer. Oncogene. 32(11):1351-62.

Li Y et. al. (January 2013). Epigenetic regulation of multiple tumor-related genes leads to suppression of breast tumorigenesis by dietary genistein. PLoS One. 8(1):e54369.

Zou Y et. al. (July 2012). Effect of phenylhexyl isothiocyanate on aberrant histone H3 methylation in primary human acute leukemia. J Hematol Oncol. 5:36.

Dong C et. al. (April 2012). G9a interacts with Snail and is critical for Snail-mediated E-cadherin repression in human breast cancer. J Clin Invest. 122(4):1469-86.

Candelaria M et. al. (March 2012). DNA methylation-independent reversion of gemcitabine resistance by hydralazine in cervical cancer cells. PLoS One. 7(3):e29181.

Chestnut BA et. al. (November 2011). Epigenetic regulation of motor neuron cell death through DNA methylation. J Neurosci. 31(46):16619-36.