EpiQuik Histone Methyltransferase Activity/Inhibition Assay Kit (H3K27)

The EpiQuik™ Histone Methyltransferase Activity/Inhibition Assay Kit (H3-K27) is a convenient set of tools that allows the experimenter to measure the activity or inhibition of individual histone methyltransferase that specifically target histone H3 at lysine 27 (H3-K27). The kit is ready-to-use and provides all the essential components needed to carry out a successful HMT activity/inhibition experiment without the need for radioactivity or any special equipment. The kit has the following advantages:

• Very rapid procedure, which can be completed within 3 hours.
• Safe and innovative colorimetric assay without radioactivity, electrophoresis, and chromatography.
• Specific measurement of activity/inhibition of H3-K27 histone methyltransferases.
• Strip microplate format makes the assay flexible: manual or high throughput analysis.
• Extremely simple, reliable, and consistent assay conditions.

Principle & Procedure
The EpiQuik™ Histone Methyltransferase Activity/Inhibition Assay Kit (H3-K27) is designed for measuring HMTs that specifically target histone H3 at lysine 27. In an assay with this kit, the histone substrate is stably captured on the strip wells through biotin-streptavidin binding. HMT enzymes transfer a methyl group to histone H3 substrate from Adomet to methylate the substrate at lysine 4. The methylated histone H3-K27 can be recognized with a high-affinity antibody. The ratio or amount of methylated H3- K27, which is directly proportional to enzyme activity, can be quantified through a HRP conjugated secondary antibody-color development system. The HMT activity is then calculated based on the amount of methylated H3-K27 converted by the HMTs.