The EpiQuik™ Histone Methyltransferase Activity/Inhibition Assay Kit (H3-K27) is a convenient set of tools that allows the experimenter to measure the activity or inhibition of individual histone methyltransferase that specifically target histone H3 at lysine 27 (H3-K27). The kit is ready-to-use and provides all the essential components needed to carry out a successful HMT activity/inhibition experiment without the need for radioactivity or any special equipment. The kit has the following advantages:
Principle & ProcedureThe EpiQuik™ Histone Methyltransferase Activity/Inhibition Assay Kit (H3-K27) is designed for measuring HMTs that specifically target histone H3 at lysine 27. In an assay with this kit, the histone substrate is stably captured on the strip wells through biotin-streptavidin binding. HMT enzymes transfer a methyl group to histone H3 substrate from Adomet to methylate the substrate at lysine 4. The methylated histone H3-K27 can be recognized with a high-affinity antibody. The ratio or amount of methylated H3- K27, which is directly proportional to enzyme activity, can be quantified through a HRP conjugated secondary antibody-color development system. The HMT activity is then calculated based on the amount of methylated H3-K27 converted by the HMTs.
Fig. 1. Schematic procedure for using the EpiQuik Histone Methyltransferase Activity/Inhibition Assay Kit (H3K27).
Fig. 2. Nuclear extracts were prepared from MCF-7 cells using the EpiQuik Nuclear Extraction Kit and H3-K27 specific histone methyltrasferase activity was measured.
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