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EpiQuik DNMT Activity/Inhibition Assay Ultra Kit (Colorimetric)


For absorbance-based measurement of DNA methyltransferase activity or inhibition

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EpiQuik DNMT activity and inhibition colorimetric assay: (a) High sensitivity and specificity achieved by using recombinant DNMT1 with the EpiQuik DNMT Activity/Inhibition Assay Ultra Kit (Colorimetric); (b) High sensitivity and specificity achieved by using nuclear extract with the EpiQuik DNMT Activity/Inhibition Assay Ultra Kit (Colorimetric). Nuclear extracts were prepared from MCF-7 cells using the EpiQuik Nuclear Extraction Kit (Cat. No. OP-0002).
Schematic procedure for the EpiQuik™ DNMT Activity/Inhibition Assay Ultra Kit (Colorimetric).
Input Type: Nuclear Extracts, Purified Enzyme
Research Area: DNA Methylation
Target Application: Activity Measurement
Vessel Format: 96-Well Plate
100% Guarantee: 6 months
Catalog No.SizePriceQty
P-3009-4848 assays $349.00 
P-3009-9696 assays $579.00 
Order now & get it by Thursday, December 12th  
Product Overview

We recommend using a newer version of this product: EpiQuik DNMT Activity/Inhibition ELISA Easy Kit (Colorimetric)


The EpiQuik™ DNMT (DNA Methyltransferase) Activity/Inhibition Assay Ultra Kit is a complete set of optimized buffers and reagents that allows the experimenter to colorimetrically measure DNA methyltransferase activity or inhibition at extremely fast speeds on a 96-stripwell microplate. The kit is ready-to-use and provides all the essential components needed to carry out a successful DNMT activity/inhibition experiment without the need for radioactivity or any special equipment. The EpiQuik DNMT Activity/Inhibition Assay Ultra Kit is a further refinement of its popular predecessor kit by enhancing sample signals and significantly minimizing background signals, in addition to being five times more sensitive. The kit has the following advantages and features:

  • Colorimetric assay with easy-to-follow steps for convenience and speed. The entire procedure can be completed within 3 hours and 45 minutes.
  • Safe and innovative colorimetric assay without radioactivity, extraction, and chromatography.
  • The ultra-sensitive detection limit can be as low as 0.5 µg of nuclear extract or 0.5 ng of purified enzymes, which is five times better than the predecessor kit.
  • Optimized antibody & enhancer solutions allow high specificity to 5-mC without cross-reactivity to unmethylated cytosine.
  • Strip-well microplate format allows for either low or high throughput analysis.

The EpiQuik Nuclear Extraction Kit has also been optimized for use with this kit.

About DNA Methyltransferases
The addition of methyl groups is carried out by a family of enzymes, DNA methyltransferases (DNMTs or DNA MTases). Chromatin structure in the vicinity of gene promoters also affects DNA methylation and transcriptional activity. DNMT1, DNMT3A, and DNMT3B are required for the establishment and maintenance of DNA methylation patterns. Two additional enzymes, DNMT2/TRDMT1 and DNMT3L, may also have more specialized but related functions. DNMT1 appears to be responsible for maintenance of established patterns of DNA methylation, while DNMT3A and 3B seem to mediate establishment of new or de novo, DNA methylation patterns. DNMT2/TRDMT1 was shown to methylate tRNAAsp at C38 and DNMT3L is found to be a catalytically inactive regulatory factor of DNA methyltransferases, which is essential for the function of DNMT3A and DNMT3B. Diseased cells such as cancer cells may be different in that DNMT1 alone is not responsible for maintaining abnormal gene hypermethylation and both DNMT1 and DNMT3B may cooperate with this function.

Product Details

Fig. 2. EpiQuik DNMT activity and inhibition colorimetric assay: (a) High sensitivity and specificity achieved by using recombinant DNMT1 with the EpiQuik DNMT Activity/Inhibition Assay Ultra Kit (Colorimetric); (b) High sensitivity and specificity achieved by using nuclear extract with the EpiQuik DNMT Activity/Inhibition Assay Ultra Kit (Colorimetric). Nuclear extracts were prepared from MCF-7 cells using the EpiQuik Nuclear Extraction Kit (Cat. No. OP-0002).

Principle & Procedure
In the non-radioactive assay with this kit, a universal DNMT substrate is stably coated onto the wells. DNMT enzymes transfer methyl groups to cytosines from Adomet to methylate the DNA substrate. The methylated DNA can be recognized with an anti-5-methylcytosine antibody. The ratio or amount of methylated DNA, which is proportional to the enzyme activity, can then be colorimetrically measured through an ELISA-like reaction by reading the absorbance in a microplate spectrophotometer at a wavelength of 450 nm. The activity of DNMT enzymes is proportional to the intensity of optical density measured.

Easy, Fast, & Flexible
The entire colorimetric assay has easy-to-follow steps for convenience and speed, which can be completed in 3 hours and 45 minutes. The stripwell microplate format allows for a flexible assay in manual or high throughput analysis. 

Safe & Convenient
All the needed reagents, including negative controls and positive controls, for measurement of DNMT activity/inhibition are conveniently packaged in the kit. The direct colorimetric quantification of DNA samples replaces obsolete or inferior methods and eliminates the need for radioactivity, extraction, or chromatography. 

Highly Sensitive & Specific
The novel procedure and proprietary kit compositions allow for an accurate measurement of DNMT activity/inhibition to be achieved with high sensitivity and specificity. The detection limit of the input DNA can be as low as 0.5 µg of nuclear extract or 0.5 ng of purified enzymes, which is five times better than the predecessor kit.

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing along with your contact information and institution name.

[Safety Data Sheet]
Product Citations

Al Sayed R et. al. (December 2019). A 2x folic acid treatment affects epigenetics and dendritic spine densities in SHSY5Y cells. Biochem Biophys Rep. 20:100681.

Sundaram MK et. al. (November 2019). Genistein Modulates Signaling Pathways and Targets Several Epigenetic Markers in HeLa Cells. Genes (Basel). 10(12)

Yen HY et. al. (November 2019). Regulation of carcinogenesis and modulation through Wnt/β-catenin signaling by curcumin in an ovarian cancer cell line. Sci Rep. 9(1):17267.

Guo W et. al. (November 2019). Homocysteine accelerates atherosclerosis by inhibiting scavenger receptor class B member1 via DNMT3b/SP1 pathway. J Mol Cell Cardiol.

Zhou L et. al. (October 2019). TET2-interacting long noncoding RNA promotes active DNA demethylation of the MMP-9 promoter in diabetic wound healing. Cell Death Dis. 10(11):813.

Awada Z et. al. (October 2019). DNA methylome-wide alterations associated with estrogen receptor-dependent effects of bisphenols in breast cancer. Clin Epigenetics. 11(1):138.

Qiu Z et. al. (October 2019). Disruption of Epigenetic Silencing in Human Colon Cancer Cells Lines Utilizing a Novel Supercritical CO<sub>2</sub> Extract of Neem Leaf (<i>Azadirachta indica</i>). Anticancer Res. 39(10):5473-5481.

Chen B et. al. (September 2019). The relationship among occupational irradiation, DNA methylation status, and oxidative damage in interventional physicians. Medicine (Baltimore). 98(39):e17373.

Sheng J et. al. (August 2019). The Inhibitory Effect of (-)-Epigallocatechin-3-Gallate on Breast Cancer Progression via Reducing <i>SCUBE2</i> Methylation and DNMT Activity. Molecules. 24(16)

Ma X et. al. (July 2019). Administration of metformin alleviates atherosclerosis by promoting H2S production via regulating CSE expression. J Cell Physiol.

Arjun HA et. al. (July 2019). Design, Synthesis, and Biological Evaluation of (E)-N'-((1-Chloro-3,4-Dihydronaphthalen-2-yl)Methylene)Benzohydrazide Derivatives as Anti-prostate Cancer Agents Front. Chem.

Sakharkar AJ et. al. (June 2019). Altered amygdala DNA methylation mechanisms after adolescent alcohol exposure contributes to adult anxiety and alcohol drinking. Neuropharmacology. :107679.

Kunovac A et. al. (June 2019). ROS promote epigenetic remodeling and cardiac dysfunction in offspring following maternal engineered nanomaterial (ENM) exposure. Part Fibre Toxicol. 16(1):24.

Izquierdo-Torres E et. al. (June 2019). Resveratrol up-regulates ATP2A3 gene expression in breast cancer cell lines through epigenetic mechanisms. Int J Biochem Cell Biol. 113:37-47.

Zhao L et. al. (June 2019). Effects of ginsenoside Rg3 on epigenetic modification in ovarian cancer cells. Oncol Rep. 41(6):3209-3218.

Zhang X et. al. (May 2019). Botulinum toxin type A prevents the phenotypic transformation of fibroblasts induced by TGF‑β1 via the PTEN/PI3K/Akt signaling pathway. Int J Mol Med.

Zhang J et. al. (May 2019). DNA methylation and regulation of DNA methyltransferases in a freeze tolerant vertebrate. Biochem Cell Biol.

Pradhan N et. al. (May 2019). Paederia foetida induces anticancer activity by modulating chromatin modification enzymes and altering pro-inflammatory cytokine gene expression in human prostate cancer cells. Food Chem Toxicol.

Kuroda T et. al. (May 2019). SALL3 expression balance underlies lineage biases in human induced pluripotent stem cell differentiation. Nat Commun. 10(1):2175.

Tian XL et. al. (May 2019). Potassium bisperoxo (1,10-phenanthroline) oxovanadate suppresses proliferation of hippocampal neuronal cell lines by increasing DNA methyltransferases. Neural Regen Res. 14(5):826-833.

Wee CW et. al. (February 2019). Radiosensitization of Glioblastoma Cells by a Novel DNA Methyltransferase-inhibiting Phthalimido-Alkanamide Derivative. Anticancer Res. 39(2):759-769.

Lewis KA et. al. (December 2018). Effects of SAHA and EGCG on Growth Potentiation of Triple-Negative Breast Cancer Cells. Cancers (Basel). 11(1)

Cramer T et. al. (December 2018). PARP Inhibitor Affects Long-term Heat-stress Response via Changes in DNA Methylation. Neuroscience. 399:65-76.

Mondal NK et. al. (November 2018). Tetrahydrocurcumin epigenetically mitigates mitochondrial dysfunction in brain vasculature during ischemic stroke. Neurochem Int. 122:120-138.

Zhang M et. al. (November 2018). Involvement of nerve growth factor in mouse hippocampal neuronal cell line (HT22) differentiation and underlying role of DNA methyltransferases. J Toxicol Environ Health A. 81(21):1116-1122.

Yang Z et. al. (November 2018). Effects of Cyclocarya paliurus polysaccharide on lipid metabolism-related genes DNA methylation in rats. Int J Biol Macromol. 123:343-349.

Wanga XZ et. al. (November 2018). Peperomin E Induces Promoter Hypomethylation of Metastatic-Suppressor Genes and Attenuates Metastasis in Poorly Differentiated Gastric Cancer. Cell Physiol Biochem. 50(6):2341-2364.

Tsai CC et. al. (November 2018). Regulation of Leptin Methylation Not via Apoptosis by Melatonin in the Rescue of Chronic Programming Liver Steatosis. Int J Mol Sci. 19(11)

Poupeau A et. al. (October 2018). Genes controlling the activation of natural killer lymphocytes are epigenetically remodeled in intestinal cells from germ-free mice. FASEB J. :fj201800787R.

Kwak J et. al. (September 2018). Hepatitis C Virus Core Protein Activates p53 to Inhibit E6-associated Protein Expression via Promoter Hypermethylation . 28(9):1007-1015.

Zuo Q et. al. (August 2018). The dietary flavone luteolin epigenetically activates the Nrf2 pathway and blocks cell transformation in human colorectal cancer HCT116 cells. J Cell Biochem.

Shahzad K et. al et. al. (August 2018). Activated protein C reverses epigenetically sustained p66Shc expression in plaque-associated macrophages in diabetes Communications Biology. 1(104)

Gao Y et. al. (July 2018). Combinational Proanthocyanidins and Resveratrol Synergistically Inhibit Human Breast Cancer Cells and Impact Epigenetic⁻Mediating Machinery. Int J Mol Sci. 19(8)

Anier K et. al. (June 2018). Cocaine-induced epigenetic DNA modification in mouse addiction-specific and non-specific tissues. Neuropharmacology.

Rudzinskas SA et. al. (June 2018). Methamphetamine Alters DNMT and HDAC Activity in the Posterior Dorsal Medial Amygdala in an Ovarian Steroid-Dependent Manner. Neurosci Lett.

Manoharlal et. al. (May 2018). Dissecting the transcriptional networks underlying the gibberellin response in Nicotiana tabacum Biol Plant. :1-16.

Luo X et. al. (May 2018). DNMT1 mediates metabolic reprogramming induced by Epstein-Barr virus latent membrane protein 1 and reversed by grifolin in nasopharyngeal carcinoma. Cell Death Dis. 9(6):619.

Xu Y et. al. (May 2018). S-allylcysteine suppresses ovarian cancer cell proliferation by DNA methylation through DNMT1. J Ovarian Res. 11(1):39.

Zhang L et al. et. al. (March 2018). Inhibition of DNMT suppresses the stemness of colorectal cancer cells through down-regulating Wnt signaling pathway Cellular Signaling.

George AK et. al. (March 2018). Exercise Mitigates Alcohol Induced Endoplasmic Reticulum Stress Mediated Cognitive Impairment through ATF6-Herp Signaling. Sci Rep. 8(1):5158.

Shahid M et. al. (March 2018). Alpha-oxoglutarate inhibits the proliferation of immortalized normal bladder epithelial cells via an epigenetic switch involving ARID1A. Sci Rep. 8(1):4505.

Søvik E et. al. (February 2018). Cocaine Directly Impairs Memory Extinction and Alters Brain DNA Methylation Dynamics in Honey Bees Front. Physiol.. 9

Li W et. al. (December 2017). Curcumin Derivative Epigenetically Reactivates Nrf2 Antioxidative Stress Signaling in Mouse Prostate Cancer TRAMP C1 Cells. Chem Res Toxicol.

Kuc C et. al. (December 2017). Rainbow trout exposed to benzo[a]pyrene yields conserved microRNA binding sites in DNA methyltransferases across 500 million years of evolution Scientific Reports. 7

Nanduri J et. al. (November 2017). DNA methylation in the central and efferent limbs of the chemo reflex requires carotid body neural activity. J Physiol.

Carden T et. al. (October 2017). Epigenetic modification of miR-663 controls mitochondria-to-nucleus retrograde signaling and tumor progression. J Biol Chem.

Fu S et. al. (September 2017). 5-Aza-2'-deoxycytidine induces human Tenon's capsule fibroblasts differentiation and fibrosis by up-regulating TGF-β type I receptor. Exp Eye Res.

Wirbisky-Hershberger SE et. al. (August 2017). Atrazine exposure decreases the activity of DNMTs, global DNA methylation levels, and dnmt expression. Food Chem Toxicol.

Li Y et. al. (August 2017). Combinatorial bioactive botanicals re-sensitize tamoxifen treatment in ER-negative breast cancer via epigenetic reactivation of ERα expression. Sci Rep. 7(1):9345.

Li et. al. (August 2017). Effects of dietary l-methionine supplementation on the growth performance, carcass characteristics, meat quality, and muscular antioxidant capacity and myogenic gene expression in low birth weight pigs J. Anim. Sci..

Xu Y et. al. (July 2017). Intracellular adenosine regulates epigenetic programming in endothelial cells to promote angiogenesis. EMBO Mol Med.

Xu H et. al. (May 2017). DNMT1 regulates IL-6- and TGF-β1-induced epithelial mesenchymal transition in prostate epithelial cells. Eur J Histochem. 61(2):2775.

Yin J et. al. (April 2017). Acylamino acid chiral fungicides on toxiciepigenetics in lambda DNA methylation. Food Chem Toxicol.

Ignácio ZM et. al. (March 2017). Quetiapine treatment reverses depressive-like behavior and reduces DNA methyltransferase activity induced by maternal deprivation. Behav Brain Res. 320:225-232.

Fan Yang et. al. (December 2016). Casticin inhibits the activity of transcription factor Sp1 and the methylation of RECK in MGC803 gastric cancer cells Experimental and Therapeutic Medicine. 13(2):745-750.

Sanchez OF et. al. (December 2016). Lead (Pb) exposure reduces global DNA methylation level by non-competitive inhibition and alteration of dnmt expression. Metallomics.

Vibe Nylander et. al. (September 2016). Ionizing Radiation Potentiates High Fat Diet-Induced Insulin Resistance and Reprograms Skeletal Muscle and Adipose Progenitor Cells ADA. 65(10)

Herencia C et. al. (June 2016). Procaine Inhibits Osteo/Odontogenesis through Wnt/β-Catenin Inactivation. PLoS One. 11(6):e0156788.

Wijenayake S et. al. (February 2016). The role of DNA methylation during anoxia tolerance in a freshwater turtle (Trachemys scripta elegans). J Comp Physiol B.

Poh WJ et. al. (January 2016). DNA Methyltransferase Activity Assays: Advances and Challenges Theranostics. 6(3):369-391.

Sun R et. al. (November 2015). Deoxyribonucleic acid (DNA) methyltransferase contributes to p16 promoter CpG island methylation in lung adenocarcinoma with smoking Int J Clin Exp Med. 8(9):15773-15779.

Naselli F et. al. (October 2015). Phytochemical Indicaxanthin Inhibits Colon Cancer Cell Growth and Affects the DNA Methylation Status by Influencing Epigenetically Modifying Enzyme Expression and Activity. J Nutrigenet Nutrigenomics. 8(3):114-27.

Pushpakumar S et. al. (July 2015). DNA hypermethylation in hyperhomocysteinemia contributes to abnormal extracellular matrix metabolism in the kidney. FASEB J.

Li JJ et. al. (June 2015). Homocysteine Triggers Inflammatory Responses in Macrophages through Inhibiting CSE-H2S Signaling via DNA Hypermethylation of CSE Promoter. Int J Mol Sci. 16(6):12560-12577.

Pathania R et. al. (April 2015). DNMT1 is essential for mammary and cancer stem cell maintenance and tumorigenesis. Nat Commun. 6:6910.

Zeng F et. al. (March 2015). Dynamic change of DNA methylation and cell redox state at different micropropagation phases in birch. Trees. :1-14.

Ishikawa K et. al. (February 2015). Long-term pancreatic beta cell exposure to high levels of glucose but not palmitate induces DNA methylation within the insulin gene promoter and represses transcriptional activity. PLoS One. 10(2):e0115350.

Bian Y et. al. (February 2015). Epigenetic Regulation of miR-29s Affects the Lactation Activity of Dairy Cow Mammary Epithelial Cells. J Cell Physiol.

Cho SH et. al. (January 2015). SIRT1 deficiency in microglia contributes to cognitive decline in aging and neurodegeneration via epigenetic regulation of IL-1β. J Neurosci. 35(2):807-18.

Cheng C et. al. (December 2014). 'Default' generated neonatal regulatory T cells are hypomethylated at conserved non-coding sequence 2 and promote long-term cardiac allograft survival. Immunology. 143(4):618-30.

Zhang S et. al. (December 2014). The Effect of Hypoxia Preconditioning on DNA Methyltransferase and PP1γ in Hippocampus of Hypoxia Preconditioned Mice. High Alt Med Biol. 15(4):483-90.

Cardenas H et. al. (November 2014). TGF-β induces global changes in DNA methylation during the epithelial-to-mesenchymal transition in ovarian cancer cells. Epigenetics.

Chen S et. al. (November 2014). Identifying Novel Selective Non-Nucleoside DNA Methyltransferase 1 Inhibitors through Docking-Based Virtual Screening. J Med Chem. 57(21):9028-41.

Choi YW et. al. (November 2014). B-RafV600E inhibits sodium iodide symporter expression via regulation of DNA methyltransferase 1. Exp Mol Med. 46:e120.

Wang L et. al. (November 2014). Blocking of JB6 Cell Transformation by Tanshinone IIA: Epigenetic Reactivation of Nrf2 Antioxidative Stress Pathway. AAPS J. 16(6):1214-25.

Pudenz M et. al. (October 2014). Impact of soy isoflavones on the epigenome in cancer prevention. Nutrients. 6(10):4218-72.

Zhu Y et. al. (September 2014). Placental mesenchymal stem cells of fetal origin deposit epigenetic alterations during long-term culture under serum-free condition. Expert Opin Biol Ther. :1-18.

Lai J et. al. (August 2014). Transcriptional regulation of the p73 gene by Nrf-2 and promoter CpG methylation in human breast cancer. Oncotarget. 5(16):6909-22.

Wang J et. al. (July 2014). MicroRNA-152 Regulates DNA Methyltransferase 1 and Is Involved in the Development and Lactation of Mammary Glands in Dairy Cows. PLoS One. 9(7):e101358.

Sakharkar AJ et. al. (June 2014). Effects of acute ethanol exposure on anxiety measures and epigenetic modifiers in the extended amygdala of adolescent rats. Int J Neuropsychopharmacol. :1-11.

Scola G et. al. (April 2014). Lithium reduces the effects of rotenone-induced complex I dysfunction on DNA methylation and hydroxymethylation in rat cortical primary neurons. Psychopharmacology (Berl).

Park J et. al. (April 2014). Hepatitis C virus represses E-cadherin expression via DNA methylation to induce epithelial to mesenchymal transition in human hepatocytes. Biochem Biophys Res Commun. 446(2):561-7.

Chaturvedi P et. al. (April 2014). Differential regulation of DNA methylation versus histone acetylation in cardiomyocytes during HHcy in vitro and in vivo: an epigenetic mechanism. Physiol Genomics. 46(7):245-55.

Lin N et. al. (April 2014). Homocysteine induces cytotoxicity and proliferation inhibition in neural stem cells via DNA methylation in vitro. FEBS J. 281(8):2088-96.

Wu J et. al. (March 2014). Kaposi's sarcoma-associated herpesvirus (KSHV) vIL-6 promotes cell proliferation and migration by upregulating DNMT1 via STAT3 activation. PLoS One. 9(3):e93478.

Wang Y et. al. (March 2014). Ras-induced epigenetic inactivation of Ras-related associated with diabetes gene (RRAD) promotes glucose uptake in a human ovarian cancer model. J Biol Chem.

Zhang X et. al. (February 2014). Regulation of DNA methylation by ethanol induces tissue plasminogen activator expression in astrocytes. J Neurochem. 128(3):344-9.

Muren NB et. al. (November 2013). Electrochemical assay for the signal-on detection of human DNA methyltransferase activity. J Am Chem Soc. 135(44):16632-40.

Liao W et. al. (November 2013). Enhancing bone marrow regeneration by SALL4 protein. J Hematol Oncol. 6:84.

Lee H et. al. (August 2013). DNA methyltransferase inhibitor assay system based on the HBx-induced DNA methylation of E-cadherin. Biochem Biophys Res Commun. 438(3):540-5.

Williams-Karnesky RL et. al. (August 2013). Epigenetic changes induced by adenosine augmentation therapy prevent epileptogenesis. J Clin Invest. 123(8):3552-63.

Chen Z et. al. (July 2013). Epigenetic regulation of cardiac progenitor cells marker c-kit by stromal cell derived factor-1α. PLoS One. 8(7):e69134.

Yara S et. al. (May 2013). Total parenteral nutrition induces sustained hypomethylation of DNA in newborn guinea pigs. Pediatr Res. 73(5):592-5.

Kauntz H et. al. (April 2013). Epigenetic effects of the natural flavonolignan silibinin on colon adenocarcinoma cells and their derived metastatic cells. Oncol Lett. 5(4):1273-1277.

Gao F et. al. (October 2012). Differential DNA methylation in discrete developmental stages of the parasitic nematode Trichinella spiralis. Genome Biol. 13(10):R100.

Tewari S et. al. (July 2012). Mitochondria DNA replication and DNA methylation in the metabolic memory associated with continued progression of diabetic retinopathy. Invest Ophthalmol Vis Sci. 53(8):4881-8.

Yang J et. al. (January 2012). Stem cell gene SALL4 suppresses transcription through recruitment of DNA methyltransferases. J Biol Chem. 287(3):1996-2005.

Customer Reviews

Rating by k******* Unverified Purchase Reviewed on: Tuesday 13 March, 2018
Application Description
I use this assay for cancer chemotherapeutic purposes to determine the efficacy of my drugs to impact the epigenome.

Procedural Details
The procedure is fairly straight forward. I have noticed that following specific steps can be a bit challenging for my foreign colleagues due to wording choices in the protocol.

Other Thoughts
I wish the different buffers/Abs/etc. were shipped at lower concentrations. Sometimes I only need to use 24-36 of the wells, which makes for awkward dilution quantities.

Small experimental set-up

All of the diluted tubes...there’s a lot!
Rating by 2******** Unverified Purchase Reviewed on: Tuesday 22 August, 2017
Application Description
This product is for determine the DNMT activity, the price is great, with this perfect product
Rating by L** Verified Purchase Reviewed on: Monday 19 December, 2016
Application Description
We used EpiQuik DNMT Activity/Inhibition Assay Ultra Kit (p-3009) and EpiQuik HDAC Activity/Inhibition Assay Kit (p-4002) to check DNMT and HDAC activity in tumor cells.

Pros: They are very easy to work with. Protocols are clearly delineated the detailed procedures.
Cons: Too much steps and solutions, which may cause confusion and mistakes.

Other Thoughts
1) DNMT kit should have positive control included.
2) There is a concern for how to determine internal HDAC and DNMT quantity (protein level) to normalize all samples.

How friendly was the user guide? (1 – worst, 5 – best)

How professional was the appearance and presentation of the product? (1 – worst, 5 – best)

How would you rate the overall product? (1 – worst, 5 – best)
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