Targeted Bisulfite Sequencing (Methyl-Seq) Service


Targeted Bisulfite Sequencing is an NGS method used to evaluate site-specific DNA methylation changes. The process is similar to pyrosequencing but offers a much higher throughput overall. Our next-generation sequencing platforms can deliver a great amount of useful DNA methylation information with publication-ready data parsed by our expert bioinformatics scientists. The methylation analysis at single-base resolution of individual cytosine in DNA is facilitated by bisulfite treatment of DNA followed by PCR amplification of targeted region, library construction, and sequencing of the amplicon regions. Specific primers are designed for the region of interest and cytosine methylation changes are evaluated within that region. Each DNA methylation site of interest receives a high-sequencing depth of coverage for accurate, quantitative and single-base resolution data output.

EpiGentek’s Targeted Methyl-Seq platform yields reliable information on the methylation states of individual cytosines within the targeted region by effectively and efficiently preparing converted DNA for use in next-generation sequencing techniques.

Why Targeted Bisulfite-Seq With Us?
Service #S-1TMS
Bisulfite sequencing method to detect base resolution DNA methylation at a targeted region of interest
End-to-End Package:
Includes everything from sample preparation to data analysis
Bioinformatics:
FASTQ raw data, FASTQC quality control insights, read mapping, methylation calling and differential methylation analysis
Rapid Turnaround Time:
4 – 8 weeks from sample submission*
Sequencing & Library Preparation Guarantee:
Library QC by Bioanalyzer, KAPA Quantification
and Q30 Sequencing score > 75%
High Sequencing Depth:
Each base can be covered > 100X
Post-Completion Technical Support:
Complimentary high-quality support to assist with data analysis at no additional charge

*Time measured when pre-payment for service is received

**Assuming 12 isolated DNA samples submitted,
1 primer pair and placed on 1 SR50 lane

What EpiGentek Provides:

Example Data

Targeted Bisulfite-Seq (Methyl-Seq) Example Data & Figures
*These are examples of data that may be provided. Please consult your service representative regarding the exact data you will receive.


Sample Requirements

Epigenetic Services Sample Requirements

We currently accept DNA, cell, and tissue samples for our Targeted Bisulfite Sequencing Methyl-Seq service. Proper sample preparation along with the appropriate quality control methods on your end will allow the greatest chance for assay success. Please follow all guidelines for sample requirements, packaging and labeling, and shipping which are outlined on the

Submission Instructions

page.

Talk to a Scientist

talk-to-a-scientist2

Want more information on our Targeted Bisulfite Sequencing Methyl-Seq service or interested in customizing your research project? We’re here to help! Get in touch with one of our highly knowledgeable epigenetic services experts by calling toll-free at 1-877-374-4368 or emailing services @ epigentek.com.