Description Mouse monoclonal antibody to 5-methylcytosine (5-mC), clone 33D3, MeDIP/ChIP-grade Concentration 1 mg/ml Purification Protein A Immunogen Ovalbumin-conjugated 5-methylcytosine (5-mC) Isotype IgG1 Specificity Modified base 5-methylcytosine (5-mC), a broad range of species Formulation Purified IgG in 10 mM phosphate buffer, 150 mM NaCl, pH 7.4 Storage 4°C, stable for 6 months from the date of shipment. For long-term storage, aliquot and store at -20°C. Avoid repeated freezing and thawing. Multiple freeze/thaw cycles may result in decreased performance. Handling Recommendations For maximum recovery of the products, centrifuge the vial prior to opening the cap. Alternate Names 5-methylcytidine, anti-5-methylcytidine, anti-5-methylcytosine, 5mC, 5meC, 5-meC Application & Suggested Dilutions* Dot Blot: 1:1000-1:2000; Immunohistochemistry: 1:100-1:500; Immunofluorescence: 1:100-1:500; ELISA: 1:1000-1:2000; MeDIP: 0.5-1 µg/reaction *The end user is responsible for determining optimal working dilutions.

Fig. 1. Methylated DNA successfully captured by anti-5-methylcytosine clone 33D3 (Cat #A-1014) during MeDIP. Fig. 2. Immunofluorescence staining with anti-5-methylcytosine clone 33D3 (Cat #A-1014).

[User Guide]* *Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

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