Epigentek Home
EpiGentek Guarantee

EpiQuik Methyl-CpG Binding Domain Protein 2 ChIP Kit


Write a Review
Suggested Workflow
Chromatin Isolation
Chromatin Shearing
MDB Protein ChIP
PCR Analysis
Schematic procedure for using the EpiQuik Methyl-CpG Binding Domain Protein 2 ChIP Kit.
Input Type: Chromatin
Research Area: Chromatin & Transcription
Target Application: Immunoprecipitation
Vessel Format: 96-Well Plate
100% Guarantee: 6 months
Catalog No.SizePriceQty
P-2017-2424 reactions $399.00 
P-2017-4848 reactions $579.00 
Availability: Usually Ships In 1-2 Days 
Product Overview

The EpiQuik™ Methyl-CpG Binding Domain Protein 2 ChIP Kit is a convenient package of tools that allows the experimenter to investigate the binding of MBD2 to methylated DNA in vivo efficiently. The entire procedure can be completed within 5 hours and produces far superior results than any competitor kits. The EpiQuik™ Methyl-CpG Binding Domain Protein 2 ChIP Kit is suitable for combining the specificity of immunoprecipitation with qualitative and quantitative PCR, MS-PCR, DNA sequencing, and southern blot as well as DNA microarray. The kit has the following advantages:

  • The fastest procedure available, which can be finished within 5 hours.
  • Strip microwell format makes the assay flexible: manual or high throughput.
  • Columns for DNA purification are included: save time and reduce labor.
  • Compatible with all DNA amplification-based approaches.
  • Simple, reliable, and consistent assay conditions.

Principle & Procedure
The EpiQuik™ Methyl-CpG Binding Domain Protein 2 ChIP Kit contains all reagents required for carrying out a successful chromatin immunoprecipitation for MBD2 from mammalian cells. Particularly, this kit includes a ChIP-grade MBD2 antibody and a negative control normal mouse IgG. Chromatin from the cells is extracted, sheared and added into the microwell immobilized with the antibody. DNA is released from the antibody-captured MBD2 protein-DNA complex, reversed and purified through the specifically designed Fast-Spin Column. Eluted DNA can be used for various down-stream applications.

Product Components

CP1 (Wash Buffer) 
CP2 (Antibody Buffer)
CP3A (Pre-Lysis Buffer)
CP3B Lysis Buffer)
CP4 (ChIP Dilution Buffer)
CP5 (DNA Release Buffer)
CP6 (Reverse Buffer)
CP7 (Binding Buffer)
CP8 (Elution Buffer)
Protease Inhibitor Cocktail (100X)*
Proteinase K (10 mg/ml)*
Normal Mouse IgG (0.5 mg/ml)*
Anti-MBD2 (0.2 mg/ml)*
8-Well Assay Strips (with Frame)
8-Well Strip Caps
F-Spin Column
F-Collection Tube
User Guide

* For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap.

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Safety Data Sheet]
MethylFlash ELISA Easy
Related Products

Researchers Also Purchased
DNMT1 Monoclonal Antibody [60B1220.1]
DNMT1 Monoclonal Antibody [60B1220.1]
EpiQuik Methylated DNA Immunoprecipitation Kit
EpiQuik Methylated DNA Immunoprecipitation Kit
MethylFlash Global DNA Hydroxymethylation (5-hmC) ELISA Easy Kit (Colorimetric)
MethylFlash Global DNA Hydroxymethylation (5-hmC) ELISA Easy Kit (Colorimetric)
HRP-Goat Anti-Rabbit Secondary Antibody
HRP-Goat Anti-Rabbit Secondary Antibody
Terms & Conditions | Privacy Policy | Site Map
Copyright © 2019 EpiGentek Group Inc. All rights reserved.