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EpiQuik Nuclear Extraction Kit II (Nucleic Acid-Free)

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For isolation of nucleic acid-free nuclear proteins from cells or tissues

Citations (8) | Write a Review
Schematic image of the EpiQuik™ Nuclear Extraction Kit II (Nucleic Acid-Free).
Input Type: Unisolated Samples
Research Area: DNA Methylation, Histone Acetylation, Histone Methylation, Sumoylation
Target Application: Sample Isolation
Vessel Format: Columns/Tubes
100% Guarantee: 6 months
Catalog No.SizePriceQty
OP-0022-100100 extractions $158.00 
Availability: Usually Ships in 1 Day or Same day delivery Same Day NY Delivery 
Product Overview

The EpiQuik™ Nuclear Extraction Kit II (Nucleic Acid-Free) is a complete set of optimized reagents to provide a simple and selective method for isolating nuclear proteins free of nucleic acids attached, to be used in a variety of applications in just 60 minutes. These applications may include Western blot, protein-DNA binding assays, nuclear enzyme assays, and any other procedures requiring optimized nucleic acid-free nuclear proteins but not enzymatic activity. The protocol is fast and easy-to-use, and also isolates very abundant yields of nuclear extract from mammalian cells or tissue samples. Some of the kit's highlighted featured include the following:

  • Pre-optimized and simple step-wise 1 hour protocol.
  • Conveniently includes all essential reagents to carry out a nucleic acid-free nuclear extraction.
  • Standardized procedure for reproducible results.

Principle & Procedure
The EpiQuik™ Nuclear Extraction Kit II (Nucleic Acid-Free) simply applies our proprietary nuclear protein isolation buffers to cell/tissues. After treatment with pre-lysis and lysis buffers, the nucleic acid-free nuclear proteins are easily extracted for immediate use or storage at proper conditions.

Note: This item was formerly catalog no. OP-0002-2 (OP00022, OP-00022, OP0002-2)

 

 
Fig. 1. Schematic image of the EpiQuik™ Nuclear Extraction Kit II (Nucleic Acid-Free).

Product Components

NP1 (10X Pre-Extraction Buffer)
NP2 (Extraction Buffer)
NP3 (Extraction Pre-Cleaner)
NP4 (Extraction Cleaner)
1000X DTT Solution
1000X Protease Inhibitor Cocktail (PIC)
User Guide

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Material Safety Data Sheet]
Product Citations

Yana H et. al. (December 2015). The effects of LSD1 inhibition on self-renewal and differentiation of human induced pluripotent stem cells Exp Cell Res.

Haupt S et. al. (November 2015). Alternative promoter methylation regulates together with Foxp3 transcription of GARP. Biochim Biophys Acta.

Ivankovic D et. al. (October 2015). Mitochondrial and lysosomal biogenesis are activated following PINK1/parkin-mediated mitophagy. J Neurochem.

Wessam F. El-Hadidy et. al. (April 2014). Possible protective effect of procainamide as an epigenetic modifying agent in experimentally induced type 2 diabetes mellitus in rats. Alexandria Journal of Medicine. S2090-5068(14):00028-1. Abstract

Siuda D et. al. (April 2014). Social isolation-induced epigenetic changes in midbrain of adult mice. J Physiol Pharmacol. 65(2):247-55.

Yamashita K et. al. (March 2014). The effects of the heme precursor 5-aminolevulinic acid (ALA) on REV-ERBĪ± activation. FEBS Open Bio. 4:347-52.

Wei W et. al. (May 2013). Codelivery of mTERT siRNA and paclitaxel by chitosan-based nanoparticles promoted synergistic tumor suppression. Biomaterials. 34(15):3912-23.

Galoian K et. al. (June 2012). mTORC1 inhibition and ECM-cell adhesion-independent drug resistance via PI3K-AKT and PI3K-RAS-MAPK feedback loops. Tumour Biol. 33(3):885-90.

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