The EpiQuik™ In Vivo Universal Protein Sumoylation Assay Kit is a convenient set of tools that allows the experimenter to measure in vivo protein sumoylation. The kit is ready-to-use and provides all the essential components needed for measuring in vivo protein sumoylation from multiple mammalian cells/tissues including human, mouse and rat.

WHY CHOOSE THE EPIQUIK™ IN VIVO UNIVERSAL PROTEIN SUMOYLATION ASSAY KIT?

• Fast procedure, which can be finished within 5 hours.
• One-step colorimetric assay without the need for affinity chromatography and Western blotting.
• Flexible antibody choice allows the detection of sumoylation of multiple target proteins simultaneously.
• Included SUMO protein as the positive control allows protein sumoylation to be quantified.
• Strip microplate format makes the assay flexible: manual or high throughput analysis.
• Simple, reliable, and consistent assay conditions.

The EpiQuik™ In Vivo Universal Protein Sumoylation Assay Kit is designed for measuring sumoylation of targeted proteins. Sumoylation of the targeted proteins is indicated by SUMO conjugated to these proteins. In an assay with this kit, the antibodies specific to the targeted proteins are stably bound to the strip wells and the targeted proteins are captured by these antibodies. Sumoylation of the targeted proteins is detected by recognition of SUMO conjugated to these proteins with an anti-SUMO antibody. The ratio or intensity of the sumoylation, which is proportional to the conjugated SUMO amount, can be quantified through the signal report-color development system.

SCHEMATIC PROCEDURE:

S1 (10X Wash Buffer)
S2 (Binding Buffer)
S3 (Mouse IgG, 1 µg/µl)*
S4 (Blocking Buffer)
S5 (Sumo Assay Buffer)
S6 (Sumo Protein, 1 µg/µl)*
S7 (Sumo Antibody, 1 µg/µl)*
S8 (Signal Report Solution)*
S9 (Color Development Solution)
S10 (Stop Solution)
8-Well Assay Strip (with Frame)
8-Well Control Strips
User Guide


* For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap.

[User Guide]* *Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

Gao, G. et. al. (November 2010). Proteasome Activator REG{gamma} Enhances Coxsackieviral Infection by Facilitating p53 Degradation. J Virol. 84(21): 11056-66. PubMed Abstract