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EpiQuik Global Acetyl Histone H3K9 Quantification Kit (Colorimetric)

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For ELISA-like measurement of total H3K9ac amounts

Citations (9) | Write a Review
Suggested Workflow
Histone Protein Extraction
 
 
Histone H3 Acetylation Quantification
 
Schematic procedure for using the EpiQuik Global Acetyl Histone H3K9 Quantification Kit (Colorimetric).
Input Type: Histone Extracts
Research Area: Histone Acetylation
Target Application: Amount Quantitation
Vessel Format: 96-Well Plate
100% Guarantee: 6 months
Catalog No.SizePriceQty
P-4010-4848 assays $277.00 
P-4010-9696 assays $499.00 
Availability: Usually Ships In 1-2 Days 
Product Overview

The EpiQuik™ Global Acetyl Histone H3-K9 Quantification Kit (Colorimetric) is a convenient package of tools that allows the experimenter to specifically measure global acetylation of histone H3-K9 colorimetrically, using a variety of mammalian cells (human, mouse, etc.) including fresh and frozen tissues, and cultured adherent and suspension cells.  The kit has the following advantages:

  • Quick and efficient procedure, which can be finished within 2.5 hours.
  • Innovative colorimetric assay without the need for radioactivity, electrophoresis, or chromatography.
  • Specifically captures acetyl H3-K9 with the detection limit as low as 2 ng/well and detection range from 20 ng-5 µg/well of histone extracts.
  • The control is conveniently included for the quantification of the amount of acetyl H3-K9.
  • Strip microplate format makes the assay flexible: manual or high throughput.
  • Simple, reliable, and consistent assay conditions.

Background Information
Acetylation of histones, including histone H3, has been involved in the regulation of chromatin structure and recruitment of transcription factors to the gene promoters. Histone acetyltransferases (HATs) and histone deacetylases (HDACs) play a critical role in the control of histone H3 acetylation at multiple sites. Histone H3 at lysine 9 (H3-K9) is a primary acetylated site of histone H3. Acetylation of histone H3-K9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Acetylation of histone H3-K9 is tightly involved in cell cycle regulation, cell proliferation, and apoptosis; acetylation of histone H3-K9 is also an active marker. The balance between histone H3-K9 acetylation and methylation is important for the establishment of specific chromatin structures. An imbalance in the equilibrium of histone H3 acetylation, including K9 acetylation, has been associated with tumorigenesis and cancer progression. Histone H3-K9 acetylation may be increased by inhibition of HDACs and decreased by HAT inhibition. Thus, quantitative detection of global acetyl histone H3-K9 would provide useful information for better understanding epigenetic regulation of gene activation and for developing HAT or HDAC-targeted drugs. The EpiQuik™ Global Acetyl Histone H3-K9 Quantification Kit (Colorimetric) provides a tool for measuring global acetylation of histone H3-K9.

Principle & Procedure
This kit is designed for measuring global histone H3-K9 acetylation. In an assay with this kit, the acetyl histone H3 at lysine 9 is captured to the strip wells coated with an anti-acetyl H3-K9 antibody. The captured acetyl histone H3-K9 can then be detected with a labeled detection antibody followed by a color development reagent. The ratio of acetyl H3-K9 is proportional to the intensity of absorbance. The absolute amount of acetyl H3-K9 can be quantified by comparing to the standard control.

Starting Materials
Input material should be purified histone extracts. In general, the input amount should be from 50 ng to 200 ng per well of histone extracts. 

 

Product Components

C1 (10X wash buffer)
C2 (antibody buffer)
C3 (detection antibody, 1 mg/ml)*
C4 (color developer)
C5 (stop solution)
Standard control (100 µg/ml)*
8 well sample strips (with frame)
8 well standard control strips
User guide

* For maximum recovery of the products, centrifuge the original vial prior to opening the cap.

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Material Safety Data Sheet]
Product Citations

Ding R et. al. (March 2017). Dose- and time- effect responses of DNA methylation and histone H3K9 acetylation changes induced by traffic-related air pollution. Sci Rep. 7:43737.

Ding R et. al. (January 2016). H3K9 acetylation change patterns in rats after exposure to traffic-related air pollution. Environ Toxicol Pharmacol. 42:170-175.

Kumar P et. al. (June 2014). All-trans retinoic acid and sodium butyrate enhance natriuretic peptide receptor a gene transcription: role of histone modification. Mol Pharmacol. 85(6):946-57.

Cantone L et. al. (April 2014). Extracellular histones mediate the effects of metal-rich air particles on blood coagulation. Environ Res. 132C:76-82.

Siuda D et. al. (April 2014). Social isolation-induced epigenetic changes in midbrain of adult mice. J Physiol Pharmacol. 65(2):247-55.

Kumar P et. al. (March 2014). Histone deacetylase inhibitors modulate the transcriptional regulation of guanylyl cyclase/natriuretic peptide receptor-a gene: interactive roles of modified histones, histone acetyltransferase, p300, AND Sp1. J Biol Chem. 289(10):6991-7002.

Monteiro JB et. al. (March 2014). Endometriosis is characterized by a distinct pattern of histone 3 and histone 4 lysine modifications. Reprod Sci. 21(3):305-18.

Shyamasundar S et. al. (June 2013). Analysis of epigenetic factors in mouse embryonic neural stem cells exposed to hyperglycemia. PLoS One. 8(6):e65945.

Deng L et. al. (May 2013). Synthesis, Activity and Metabolic Stability of Non-Ribose Containing Inhibitors of Histone Methyltransferase DOT1L. Medchemcomm. 4(5):822-826.

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