Epigentek Home
EpiGentek Guarantee

Methylamp Global DNA Methylation Quantification Ultra Kit


For ELISA-like quantitation of global DNA methylation (this kit has been replaced by #P-1030)

Citations (86) | Write a Review
Schematic procedure for the Methylamp Global DNA Methylation Quantification Ultra Kit.
Quantification of Methylated DNA.
Input Type: DNA
Research Area: DNA Methylation
Target Application: Amount Quantitation
Vessel Format: 96-Well Plate
100% Guarantee: 6 months
Catalog No.SizePriceQty
P-1014B-4848 assays $336.00 
P-1014B-9696 assays $599.00 
Availability: Usually Ships in 1 Day or Same day delivery Same Day NY Delivery 
Product Overview

We recommend using a newer version of this product: MethylFlash Global DNA Methylation (5-mC) ELISA Easy Kit (Colorimetric)

The Methylamp™ Global DNA Methylation Quantification Ultra Kit is an improved version of the popular Methylamp™ Global DNA Methylation Quantification Kit. As a complete set of essential components, the kit enables the experimenter to quantify global DNA methylation through an ELISA-like reaction using Epigentek's uniquely simplified and streamlined procedure. The entire procedure can be completed in less than 4 hours in either manual or high throughput analysis. In addition to having a shorter procedure, the improved kit also has higher sensitivty than its predecessor. It is suitable for detecting global DNA methylation status using genomic DNA isolated from variety of cultured cells, fresh and frozen tissue, paraffin-embedded tissue, plasma/serum sample, and body fluid sample, etc. The universal positive control included in the kit allows for quantitation of methylated DNA from any species.


  • Colorimetric quantification without radioactivity, extraction, and chromatography.
  • Strip microplate format makes the assay flexible: manual or high throughput analysis.


  • Shorter procedure, which can be finished in less than 4 hours.
  • Significantly higher sensitivity -- detection limitation can be as low as 0.2 ng of methylated DNA.
  • Universal positive control suitable for quantifying methylated DNA from any species.
Product Details

The Methylamp™ Global DNA Methylation Quantification Ultra Kit contains all reagents required for quantification of global DNA methylation. In this assay, DNA is immobilized to the strip well specifically coated with DNA affinity substance. The methylated fraction of DNA can be recognized by 5-methylcytosine antibody and quantified through an ELISA-like reaction. The amount of methylated DNA is proportional to the OD intensity.


Quantification of Methylated DNA.
Product Components

GU1 (10X Wash Buffer)
GU2 (DNA Binding Solution)
GU3 (Positive Control,100 µg/ml)**
GU4 (Block Solution)
GU5 (Capture Antibody, 1000 µg/ml)*
GU6 (Detecting Antibody, 400 µg/ml)*
GU7 (Enhance Solution)
GU8 (Developing Solution)
GU9 (Stop Solution)
NC (Negative Control)
8-Well Assay Strips (with Frame)
User Guide

* Spin the solution down to the bottom before use.
** This control is synthesized polynucleiotide methylated at every 5-cytosine.

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Material Safety Data Sheet]
Product Citations

Tan T et. al. (February 2018). Formaldehyde induces diabetes-associated cognitive impairments. FASEB J. :fj201701239R.

Yin M et. al. (January 2018). Micro Integral Membrane Protein (MIMP), a Newly Discovered Anti-Inflammatory Protein of Lactobacillus Plantarum, Enhances the Gut Barrier and Modulates Microbiota and Inflammatory Cytokines. Cell Physiol Biochem. 45(2):474-490.

Eleonora Gatta et. al. (August 2017). Emerging role of one-carbon metabolism and DNA methylation enrichment on δ-containing GABAA receptor expression in the cerebellum of subjects with alcohol use disorders (AUD) Int'l Journal of Neuropsychopharmacology.

Yan Ma et. al. (June 2017). Long noncoding RNA H19 inhibits the growth and invasion of trophoblasts by inactivating Wnt/β-catenin signaling via downregulation of DDX3X IJCEP. 10(6):6560-6567.

Song et. al. (June 2017). Measuring Global DNA Methylation to Assess Neurotoxicity of Titanium Dioxide Nanoparticles Science of Advanced Materials. 9(6):1051-1056.

Prasad R et. al. (May 2017). Honokiol inhibits ultraviolet radiation-induced immunosuppression through inhibition of ultraviolet-induced inflammation and DNA hypermethylation in mouse skin. Sci Rep. 7(1):1657.

Castillo S et. al. (April 2017). On the Role of Mining Exposure in Epigenetic Effects in Parkinson's Disease. Neurotox Res.

Shao Cuijie et. al. (March 2017). The regulation of chronic inflammatory persistent pain by Dnmt3a in the spinal cord in rats Biomedical Research.

Chen Wang et. al. (February 2017). Preliminary research on DNA methylation changes during murine palatogenesis induced by TCDD Journal of Cranio-Maxillofacial Surgery. 45(2)

Zhang M et. al. (February 2017). Ultraviolet B decreases DNA methylation level of CD4+ T cells in patients with systemic lupus erythematosus. Inflammopharmacology.

Chen G et. al. (January 2017). Maternal diabetes modulates dental epithelial stem cells proliferation and self-renewal in offspring through apurinic/apyrimidinicendonuclease 1-mediated DNA methylation. Sci Rep. 7:40762.

Cheon H et. al. (November 2016). Terahertz molecular resonance of cancer DNA. Sci Rep. 6:37103.

Zhang L et. al. (June 2016). Antiproliferation of berberine is mediated by epigenetic modification of constitutive androstane receptor (CAR) metabolic pathway in hepatoma cells. Sci Rep. 6:28116.

Figge DA et. al. (June 2016). Dynamic DNA Methylation Regulates Levodopa-Induced Dyskinesia. J Neurosci. 36(24):6514-24.

Liu Z et. al. (June 2016). DNA Demethylation Rescues the Impaired Osteogenic Differentiation Ability of Human Periodontal Ligament Stem Cells in High Glucose. Sci Rep. 6:27447.

Wang L et. al. (May 2016). Determining the association between methylenetetrahydrofolate reductase (MTHFR) gene polymorphisms and genomic DNA methylation level: A meta-analysis. Birth Defects Res A Clin Mol Teratol.

McClelland R et. al. (April 2016). Accelerated ageing and renal dysfunction links lower socioeconomic status and dietary phosphate intake. Aging (Albany NY).

Yamazaki M et. al. (February 2016). Fructose consumption induces hypomethylation of hepatic mitochondrial DNA in rats. Life Sci.

Huang L et. al. (February 2016). Phenanthrene exposure induces cardiac hypertrophy via reducing miR-133a expression by DNA methylation. Sci Rep. 6:20105.

Aguilar C et. al. (August 2015). DNA Methylation Dynamics Regulate the Formation of a Regenerative Wound Epithelium during Axolotl Limb Regeneration. PLoS One. 10(8):e0134791.

Nugent BM et. al. (May 2015). Brain feminization requires active repression of masculinization via DNA methylation. Nat Neurosci. 18(5):690-7.

Yamaya Y et. al. (April 2015). Methylation of free-floating deoxyribonucleic acid fragments in the bronchoalveolar lavage fluid of dogs with chronic bronchitis exposed to environmental tobacco smoke. Ir Vet J. 68(1):7.

Chen H et. al. (April 2015). Hypermethylation of glucocorticoid receptor gene promoter results in glucocorticoid receptor gene low expression in peripheral blood mononuclear cells of patients with systemic lupus erythematosus. Rheumatol Int.

Barbier E et. al. (April 2015). DNA methylation in the medial prefrontal cortex regulates alcohol-induced behavior and plasticity. J Neurosci. 35(15):6153-64.

He D et. al. (April 2015). Aberrant gene promoter methylation of p16, FHIT, CRBP1, WWOX, and DLC-1 in Epstein-Barr virus-associated gastric carcinomas. Med Oncol. 32(4):92.

Yu HL et. al. (March 2015). Global DNA methylation was changed by a maternal high-lipid, high-energy diet during gestation and lactation in male adult mice liver. Br J Nutr. :1-8.

Urdinguio RG et. al. (March 2015). Aberrant DNA methylation patterns of spermatozoa in men with unexplained infertility. Hum Reprod.

Montjean D et. al. (March 2015). Sperm global DNA methylation level: association with semen parameters and genome integrity. Andrology.

Miller MM et. al. (February 2015). Modulating DNA Methylation in Activated CD8+ T Cells Inhibits Regulatory T Cell-Induced Binding of Foxp3 to the CD8+ T Cell IL-2 Promoter. J Immunol. 194(3):990-8.

Huumonen K et. al. (September 2014). Role of microRNAs and DNA Methyltransferases in Transmitting Induced Genomic Instability between Cell Generations. Front Public Health. 2:139.

Rudolf E et. al. (September 2014). Activation of p38 and changes in mitochondria accompany autophagy to premature senescence-like phenotype switch upon chronic exposure to selenite in colon fibroblasts. Toxicol Lett. 231(1):29-37.

Franz MB et. al. (August 2014). Global and single gene DNA methylation in umbilical cord blood cells after elective caesarean: a pilot study. Eur J Obstet Gynecol Reprod Biol. 179:121-4.

Desplats P et. al. (July 2014). Epigenetic Alterations in the Brain Associated with HIV-1 Infection and Methamphetamine Dependence. PLoS One. 9(7):e102555.

Tong Z et. al. (July 2014). Age-related formaldehyde interferes with DNA methyltransferase function, causing memory loss in Alzheimer's disease. Neurobiol Aging.

Corrales J et. al. (June 2014). Effects on specific promoter DNA methylation in zebrafish embryos and larvae following benzo[a]pyrene exposure. Comp Biochem Physiol C Toxicol Pharmacol. 163:37-46.

Cheray M et. al. (June 2014). Specific inhibition of DNMT1/CFP1 reduces cancer phenotypes and enhances chemotherapy effectiveness. Epigenomics. 6(3):267-75.

Khalyfa A et. al. (May 2014). Sleep Fragmentation During Late Gestation Induces Metabolic Perturbations and Epigenetic Changes in Adiponectin Gene Expression in Male Adult Offspring Mice. Diabetes.

Li G et. al. (May 2014). F0 maternal BPA exposure induced glucose intolerance of F2 generation through DNA methylation change in Gck. Toxicol Lett. 228(3):192-199.

Batra V et. al. (April 2014). Dietary l-methionine supplementation mitigates gamma-radiation induced global DNA hypomethylation: Enhanced metabolic flux towards S-adenosyl-l-methionine (SAM) biosynthesis increases genomic methylation potential. Food Chem Toxicol. 69C:46-54.

Lin N et. al. (April 2014). Homocysteine induces cytotoxicity and proliferation inhibition in neural stem cells via DNA methylation in vitro. FEBS J. 281(8):2088-96.

El Hajj Chehadeh S et. al. (March 2014). Gestational methyl donor deficiency alters key proteins involved in neurosteroidogenesis in the olfactory bulbs of newborn female rats and is associated with impaired olfactory performance. Br J Nutr. 111(6):1021-31.

Pacaud R et. al. (March 2014). The DNMT1/PCNA/UHRF1 disruption induces tumorigenesis characterized by similar genetic and epigenetic signatures. Sci Rep. 4:4230.

Wang J et. al. (March 2014). Genome-wide screen of DNA methylation changes induced by low dose X-ray radiation in mice. PLoS One. 9(3):e90804.

Schwimmer H et. al. (February 2014). Light at night and melatonin have opposite effects on breast cancer tumors in mice assessed by growth rates and global DNA methylation. Chronobiol Int. 31(1):144-50.

Kehrmann J et. al. (January 2014). Impact of 5-Aza-2`-deoxycytidine and Epigallocatechin-3-gallate for induction of human regulatory T cells. Immunology.

Hossain MM et. al. (January 2014). Massive deregulation of miRNAs from nuclear reprogramming errors during trophoblast differentiation for placentogenesis in cloned pregnancy. BMC Genomics. 15:43.

Koufaris C et. al. (December 2013). Time and dose-dependent effects of phenobarbital on the rat liver miRNAome. Toxicology. 314(2-3):247-53.

Sable P et. al. (November 2013). Maternal micronutrients and brain global methylation patterns in the offspring. Nutr Neurosci.

Grissom NM et. al. (October 2013). Gestational overgrowth and undergrowth affect neurodevelopment: similarities and differences from behavior to epigenetics. Int J Dev Neurosci. 31(6):406-14.

Anier K et. al. (October 2013). S-adenosylmethionine modifies cocaine-induced DNA methylation and increases locomotor sensitization in mice. Int J Neuropsychopharmacol. 16(9):2053-66.

Herbstman JB et. al. (September 2013). Predictors and consequences of global DNA methylation in cord blood and at three years. PLoS One. 8(9):e72824.

Haddad R et. al. (September 2013). Cardiac structure/function, protein expression, and DNA methylation are changed in adult female mice exposed to diethylstilbestrol in utero. Can J Physiol Pharmacol. 91(9):741-9.

Kerek R et. al. (August 2013). Early methyl donor deficiency may induce persistent brain defects by reducing Stat3 signaling targeted by miR-124. Cell Death Dis. 4:e755.

Fang X et. al. (July 2013). Benzo[a]pyrene decreases global and gene specific DNA methylation during zebrafish development. Environ Toxicol Pharmacol. 36(1):40-50.

Shyamasundar S et. al. (June 2013). Analysis of epigenetic factors in mouse embryonic neural stem cells exposed to hyperglycemia. PLoS One. 8(6):e65945.

Tong Z et. al. (May 2013). Aging-associated excess formaldehyde leads to spatial memory deficits. Sci Rep. 3:1807.

Deng L et. al. (May 2013). Synthesis, Activity and Metabolic Stability of Non-Ribose Containing Inhibitors of Histone Methyltransferase DOT1L. Medchemcomm. 4(5):822-826.

Ianni M et. al. (March 2013). Genetic factors regulating inflammation and DNA methylation associated with prostate cancer. Prostate Cancer Prostatic Dis. 16(1):56-61.

Chai J et. al. (January 2013). Molecular cloning, tissue expression, and analysis with genome DNA methylation of porcine LSD1 gene. Appl Biochem Biotechnol. 169(1):134-44.

Ghadimi D et. al. (October 2012). Epigenetic imprinting by commensal probiotics inhibits the IL-23/IL-17 axis in an in vitro model of the intestinal mucosal immune system. J Leukoc Biol. 92(4):895-911.

Hsu CY et. al. (October 2012). Global DNA methylation not increased in chronic hemodialysis patients: a case-control study. Ren Fail. 34(10):1195-9.

Nielsen DA et. al. (June 2012). Forced Abstinence from Cocaine Self-Administration is Associated with DNA Methylation Changes in Myelin Genes in the Corpus Callosum: a Preliminary Study. Front Psychiatry. 3:60.

Thaler R et. al. (June 2012). DMSO is a strong inducer of DNA hydroxymethylation in pre-osteoblastic MC3T3-E1 cells. Epigenetics. 7(6):635-51.

Yin LJ et. al. (March 2012). Insufficient maintenance DNA methylation is associated with abnormal embryonic development. BMC Med. 10:26.

Wang Bx et. al. (February 2012). Overexpression of DNA damage-induced 45 α gene contributes to esophageal squamous cell cancer by promoter hypomethylation. J Exp Clin Cancer Res. 31:11.

McGuinness D et. al. (February 2012). Socio-economic status is associated with epigenetic differences in the pSoBid cohort. Int J Epidemiol. 41(1):151-60.

Yang B et. al. (January 2012). Aberrant expression of MBD genes in CD4+ T cells from patients with systemic lupus erythematosus. Lupus. 21(1):111-3.

CHEN Xiao-li et. al. (January 2012). The association between global DNA hypomethylation, disturbed methylation pattern in promoter region of mismatched repair genes and neural tube defects Chinese Journal of Evidence -Based Pediatric. 7(2):102-106. Abstract

Shearstone JR et. al. (November 2011). Global DNA demethylation during mouse erythropoiesis in vivo. Science. 334(6057):799-802.

Debien E et. al. (August 2011). ABT-737 and/or folate reverse the PDGF-induced alterations in the mitochondrial apoptotic pathway in low-grade glioma patients. Clin Epigenetics. 2(2):369-381.

Sanders YY et. al. (July 2011). Epigenetic regulation of thy-1 by histone deacetylase inhibitor in rat lung fibroblasts. Am J Respir Cell Mol Biol. 45(1):16-23.

Birkus G et. al. (May 2011). Role of cathepsin A and lysosomes in the intracellular activation of novel antipapillomavirus agent GS-9191. Antimicrob Agents Chemother. 55(5):2166-73.

Shi YY et. al. (April 2011). Diet and cell size both affect queen-worker differentiation through DNA methylation in honey bees (Apis mellifera, Apidae). PLoS One. 6(4):e18808.

Desplats P et. al. (March 2011). Alpha-synuclein sequesters Dnmt1 from the nucleus: a novel mechanism for epigenetic alterations in Lewy body diseases. J Biol Chem. 286(11):9031-7.

Chavan-Gautam P et. al. (March 2011). Gestation-dependent changes in human placental global DNA methylation levels. Mol Reprod Dev. 78(3):150.

Liu CC et. al. (February 2011). Global DNA methylation, DNMT1, and MBD2 in patients with systemic lupus erythematosus. Lupus. 20(2):131-6.

Huang SK et. al. (November 2010). Hypermethylation of PTGER2 confers prostaglandin E2 resistance in fibrotic fibroblasts from humans and mice. Am J Pathol. 177(5):2245-55.

Vucetic Z et. al. (October 2010). Maternal high-fat diet alters methylation and gene expression of dopamine and opioid-related genes. Endocrinology. 151(10):4756-64.

LaPlant Q et. al. (September 2010). Dnmt3a regulates emotional behavior and spine plasticity in the nucleus accumbens. Nat Neurosci. 13(9):1137-43.

Wang L et. al. (May 2010). Relation between hypomethylation of long interspersed nucleotide elements and risk of neural tube defects. Am J Clin Nutr. 91(5):1359-67.

Li Y et. al. (May 2010). Overexpression of the growth arrest and DNA damage-induced 45alpha gene contributes to autoimmunity by promoting DNA demethylation in lupus T cells. Arthritis Rheum. 62(5):1438-47.

Yamanaka K et. al. (February 2010). Gene silencing of DNA methyltransferases by RNA interference in bovine fibroblast cells. J Reprod Dev. 56(1):60-7.

Shutoh Y et. al. (October 2009). Low dose effects of dichlorodiphenyltrichloroethane (DDT) on gene transcription and DNA methylation in the hypothalamus of young male rats: implication of hormesis-like effects. J Toxicol Sci. 34(5):469-82.

Blazkova J et. al. (August 2009). CpG methylation controls reactivation of HIV from latency. PLoS Pathog. 5(8):e1000554.

Yaish MW et. al. (July 2009). AtMBD9 modulates Arabidopsis development through the dual epigenetic pathways of DNA methylation and histone acetylation. Plant J. 59(1):123-35.

Chen C et. al. (June 2009). Aberrant DNA methylation in thymic epithelial tumors. Cancer Invest. 27(5):582-91.

Epigentek Product Reviews
Related Products

Researchers Also Purchased
EpiQuik Global Histone H4 Acetylation Assay Kit
EpiQuik Global Histone H4 Acetylation Assay Kit
FitAmp  Blood and Cultured Cell DNA Extraction Kit
FitAmp Blood and Cultured Cell DNA Extraction Kit
EpiQuik Nuclear Extraction Kit
EpiQuik Nuclear Extraction Kit
EpiQuik DNA Methyltransferase (DNMT) Activity/Inhibition Assay Kit
EpiQuik DNA Methyltransferase (DNMT) Activity/Inhibition Assay Kit
Epigentek is an epigenetics company that specializes in epigenetic kits, antibodies, reagents, and services for epigenetic research in DNA methylation, histone modification, and chromatin studies. Terms & Conditions | Privacy Policy | Site Map
Copyright © 2017 Epigentek Group Inc. All rights reserved.