Holiday Winter Sale Holiday Winter Sale: Use code E1712WNT to save 15% on your entire order. Ends 12/29/17. » Learn More
Epigentek Home
 
EpiGentek Guarantee

EpiQuik Global Histone H4 Acetylation Assay Kit

Share:

Citations (25) | Write a Review
Suggested Workflow
Histone Protein Extraction
 
 
Histone H4 Acetylation Quantification
 
Schematic procedure for using the EpiQuik Global Histone H4 Acetylation Assay Kit.
Input Type: Histone Extracts
Research Area: Histone Acetylation
Target Application: Amount Quantitation
Vessel Format: 96-Well Plate
100% Guarantee: 6 months
Catalog No.SizePriceQty
P-4009-4848 assays $349.00 
P-4009-9696 assays $524.00 
Availability: Usually Ships In 1-2 Days 
Product Overview

TheEpiQuik™ Global Histone H4 Acetylation Assay Kit is a convenient package of tools that allows the experimenter to measure global acetylation of histone H4 at tremendously fast speeds and consistency, superior and safer than all other current methods. The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment. The EpiQuik™ Global Histone H4 Acetylation Assay Kit is suitable for specifically measuring global histone H4 acetylation using a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells. The kit has the following advantages:

  • Quick and efficient procedure, which can be finished within 5 hours.
  • Innovative colorimetric assay without the need for radioactivity, electrophoresis, or chromatography.
  • Strip microplate format makes the assay flexible: manual or high throughput analysis.
  • Simple, reliable, and consistent assay conditions.

Principle & Procedure
The EpiQuik™ Global Histone H4 Acetylation Assay Kit is designed for measuring global histone H4 acetylation. In an assay with this kit, the histone proteins are stably spotted on the strip wells. The acetylated histone H4 can be recognized with a high-affinity antibody. The ratio or amount of acetylated H4 can be quantified through HRP conjugated secondary antibody-color development system and is proportional to the intensity of color development.

Product Components

GE1 (10X lysis buffer)
GE2 (extraction buffer)
GE3 (10X wash buffer)
GE4 (histone buffer)
GE5 (blocking buffer)
GE6 (antibody buffer)
GE7 (capture antibody, 100 µg/ml)*
GE8 (detection antibody, 400 µg/ml)*
GE9 (developing solution)
GE10 (stop solution)
Acetylated histone H4 control (60 µg/ml)*
8 well assay strips (with frame)
User guide

* For maximum recovery of the products, centrifuge the original vial prior to opening the cap.

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Material Safety Data Sheet]
Product Citations

Ding S et. al. (November 2017). Chronic sun exposure is associated with distinct histone acetylation changes in human skin. Br J Dermatol.

Gilson P. Dorneles et. al. (October 2017). Acute Strenuous Exercise Induces an Imbalance on Histone H4 Acetylation/Histone Deacetylase 2 and Increases the Proinflammatory Profile of PBMC of Obese Individuals Oxidative Medicine and Cellular Longevity. 2017

Gonçalves LK et. al. (September 2017). Maternal consumption of high-fat diet and grape juice modulates global histone H4 acetylation levels in offspring hippocampus: a preliminary study. Neurosci Lett.

Li B et. al. (March 2017). DICER1 regulates endometrial carcinoma invasion via histone acetylation and methylation. J Cancer. 8(6):933-939.

Zhou K et. al. (February 2017). Aberrant histone modification in CD19<sup>+</sup> B cells of patients with chronic lymphocytic leukemia. Onco Targets Ther. 10:1173-1179.

de Mello AS et. al. (November 2016). The modulation of inflammatory parameters, Brain-derived neurotrophic factor levels and global histone H4 acetylation status in peripheral blood of patients with Gaucher disease type 1. Clin Biochem.

Cechinel LR et. al. (October 2016). Treadmill exercise induces age and protocol-dependent epigenetic changes in prefrontal cortex of Wistar rats. Behav Brain Res. 313:82-7.

Liu J et. al. (July 2016). Curcumin prevents adriamycin-induced nephropathy MCP-1 expression through blocking histone acetylation Int J Clin Exp Med. 9(7):12696-12704.

Liu XY et. al. (December 2015). Reduced Histone H3 Acetylation in CD4(+) T Lymphocytes: Potential Mechanism of Latent Autoimmune Diabetes in Adults. Dis Markers. 2015:285125.

de Moura AC et. al. (November 2015). Global Histone H4 Acetylation in the Olfactory Bulb of Lactating Rats with Different Patterns of Maternal Behavior. Cell Mol Neurobiol.

Yan N et. al. (June 2015). Histone hypoacetylation and increased histone deacetylases in peripheral blood mononuclear cells from patients with Graves' disease. Mol Cell Endocrinol.

Yang Y et. al. (March 2015). The effect of mycophenolic acid on epigenetic modifications in lupus CD4<sup>+</sup>T cells. Clin Immunol.

Cho Y et. al. (March 2014). Colon cancer cell apoptosis is induced by combined exposure to the n-3 fatty acid docosahexaenoic acid and butyrate through promoter methylation. Exp Biol Med (Maywood). 239(3):302-10.

Monteiro JB et. al. (March 2014). Endometriosis is characterized by a distinct pattern of histone 3 and histone 4 lysine modifications. Reprod Sci. 21(3):305-18.

Xiaomeng X et. al. (March 2013). Aberrant histone acetylation and methylation levels in woman with endometriosis. Arch Gynecol Obstet. 287(3):487-94.

Rehan VK et. al. (October 2012). Perinatal nicotine exposure induces asthma in second generation offspring. BMC Med. 10:129.

Ghadimi D et. al. (October 2012). Epigenetic imprinting by commensal probiotics inhibits the IL-23/IL-17 axis in an in vitro model of the intestinal mucosal immune system. J Leukoc Biol. 92(4):895-911.

Zhao M et. al. (February 2012). Abnormal epigenetic modifications in peripheral blood mononuclear cells from patients with alopecia areata. Br J Dermatol. 166(2):226-73.

Chen C et. al. (October 2011). Abnormal histone acetylation and methylation levels in esophageal squamous cell carcinomas. Cancer Invest. 29(8):548-56.

Kraft M et. al. (September 2011). Disruption of the histone acetyltransferase MYST4 leads to a Noonan syndrome-like phenotype and hyperactivated MAPK signaling in humans and mice. J Clin Invest. 121(9):3479-91.

Sui L et. al. (December 2010). Effects of perinatal hypothyroidism on regulation of reelin and brain-derived neurotrophic factor gene expression in rat hippocampus: Role of DNA methylation and histone acetylation. Steroids. 75(12):988-97.

Biermann J et. al. (January 2010). Valproic acid-mediated neuroprotection and regeneration in injured retinal ganglion cells. Invest Ophthalmol Vis Sci. 51(1):526-34.

Hu N et. al. (November 2009). Aberrant expression pattern of histone acetylation modifiers and mitigation of lupus by SIRT1-siRNA in MRL/lpr mice. Scand J Rheumatol. 38(6):464-71.

Hu N et. al. (May 2008). Abnormal histone modification patterns in lupus CD4+ T cells. J Rheumatol. 35(5):804-10.

Tsuchiya N et. al. (December 2004). Association of a functional CD19 polymorphism with susceptibility to systemic sclerosis. Arthritis Rheum. 50(12):4002-7.

Epigentek Product Reviews
Related Products

Researchers Also Purchased
EpiQuik Global Histone H3 Acetylation Assay Kit
EpiQuik Global Histone H3 Acetylation Assay Kit
Methylamp Global DNA Methylation Quantification Ultra Kit
Methylamp Global DNA Methylation Quantification Ultra Kit
MethylFlash Methylated DNA 5-mC Quantification Kit (Colorimetric)
MethylFlash Methylated DNA 5-mC Quantification Kit (Colorimetric)
EpiQuik Total Histone Extraction Kit
EpiQuik Total Histone Extraction Kit
Epigentek is an epigenetics company that specializes in epigenetic kits, antibodies, reagents, and services for epigenetic research in DNA methylation, histone modification, and chromatin studies. Terms & Conditions | Privacy Policy | Site Map
Copyright © 2017 Epigentek Group Inc. All rights reserved.