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   Home  »  Research Kits  »  Histone Methylation  »  Histone Methylation Quantification  »  EpiQuik Global Histone H3K4 Methylation Assay Kit 
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Base Cat #P-3017

EpiQuik Global Histone H3K4 Methylation Assay Kit

3.01 Review|18 Citations

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Schematic procedure for using the EpiQuik Global Histone H3K4 Methylation Assay Kit.
Input Type: Cells, Unisolated Samples
Research Area: Histone Methylation
Target Application: Amount Quantitation
Vessel Format: 96-Well Plate
100% Guarantee: 6 months
Why EpiGentek AntibodiesApplication Guarantee for Antibodies
Catalog No.SizePriceQty
P-3017-4848 assays $470.00 
P-3017-9696 assays $699.00 
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Order now & get it by Thursday, July 16th  

Product Overview

The EpiQuik™ Global Histone H3-K4 Methylation Assay Kit is suitable for specifically measuring global histone H3-K4 methylation using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells. The kit has the following advantages:

  • Quick and efficient procedure, which can be finished within 5 hours.
  • Innovative colorimetric assay without the need for radioactivity, electrophoresis, and chromatography.
  • Strip microwell format makes the assay flexible: manual or high throughput.
  • Simple, reliable, and consistent assay conditions.

Principle & Procedure
The EpiQuik™ Global H3-K4 Methylation Assay Kit is designed for measuring global histone H3-K4 methylation. In an assay with this kit, the histone proteins are stably spotted on the strip wells. The methylated histone H3-K4 can be recognized with a high-affinity antibody The ratio or amount of methylated H3-K4 can be quantified through HRP conjugated secondary antibody-color development system and is proportional to the intensity of color development.

Datasheet & SDS

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Datasheet/User Guide: P-3017.pdf If the above file is not available online, you can request a copy by contacting us.
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Customer Reviews

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Rating ★★★☆☆3/5 via Distributor/Reseller Verified Purchase
Reviewed on: Tuesday 21 November, 2017
Application Description
We analyzed the histone modifications associated with biologic drugs in different time points of the treatment following the manufacturers´ instructions. ...
  1. Crosswhite P et. al. (May 2024). TNFα Induces DNA and Histone Hypomethylation and Pulmonary Artery Smooth Muscle Cell Proliferation Partly via Excessive Superoxide Formation. Antioxidants (Basel). 13(6)
  2. Chen H et. al. (November 2023). Photoaged Polystyrene Nanoplastics Result in Transgenerational Reproductive Toxicity Associated with the Methylation of Histone H3K4 and H3K9 in Caenorhabditis elegans. Environ Sci Technol.
  3. Zhao Z et. al. (April 2023). The putative methyltransferase LaeA regulates mycelium growth and cellulase production in Myceliophthora thermophila. Biotechnol Biofuels Bioprod. 16(1):58.
  4. Ghosh et. al. (October 2021). Prevention of Inorganic Arsenic induced Squamous Cell Carcinoma of Skin in Swiss Albino Mice By Black Tea Through Epigenetic Modulation Research Square.
  5. Deniz BF et. al. (May 2021). High Gestational Folic Acid Supplementation Prevents Hypoxia-Ischemia-Induced Caspase-3 Augmenting Without Changing Synapsin And H3 Methylation Levels In The Rat Hippocampus. Int J Dev Neurosci.
  6. Padalino G et. al. (November 2020). Identification of 6-(piperazin-1-yl)-1,3,5-triazine as a chemical scaffold with broad anti-schistosomal activities. Wellcome Open Res. 5:169.
  7. Yan N et. al. (June 2019). Aberrant Histone Methylation in Patients with Graves’ Disease International Journal of Endocrinology. 2019
  8. Ding S et. al. (November 2017). Chronic sun exposure is associated with distinct histone acetylation changes in human skin. Br J Dermatol.
  9. Bilan Li et. al. (March 2017). DICER1 regulates endometrial carcinoma invasion via histone acetylation and methylation Journal of Cancer. 8:933 -939.
  10. Li B et. al. (March 2017). DICER1 regulates endometrial carcinoma invasion via histone acetylation and methylation. J Cancer. 8(6):933-939.
  11. Zhou K et. al. (February 2017). Aberrant histone modification in CD19<sup>+</sup> B cells of patients with chronic lymphocytic leukemia. Onco Targets Ther. 10:1173-1179.
  12. Yang Y et. al. (March 2015). The effect of mycophenolic acid on epigenetic modifications in lupus CD4<sup>+</sup>T cells. Clin Immunol.
  13. Zhao H et. al. (January 2014). Aberrant histone methylation in the patients with immune thrombocytopenia. Platelets. 25(3):207-10.
  14. Xiaomeng X et. al. (March 2013). Aberrant histone acetylation and methylation levels in woman with endometriosis. Arch Gynecol Obstet. 287(3):487-94.
  15. Batra V et. al. (March 2012). Interaction between γ-radiation and dietary folate starvation metabolically reprograms global hepatic histone H3 methylation at lysine 4 and lysine 27 residues. Food Chem Toxicol. 50(3-4):464-72.
  16. Zhao M et. al. (February 2012). Abnormal epigenetic modifications in peripheral blood mononuclear cells from patients with alopecia areata. Br J Dermatol. 166(2):226-73.
  17. Chen C et. al. (October 2011). Abnormal histone acetylation and methylation levels in esophageal squamous cell carcinomas. Cancer Invest. 29(8):548-56.
  18. Hu N et. al. (May 2008). Abnormal histone modification patterns in lupus CD4+ T cells. J Rheumatol. 35(5):804-10.
Rating ★★★☆☆3/5 via Distributor/Reseller Verified Purchase
Reviewed on: Tuesday 21 November, 2017
Application Description
We analyzed the histone modifications associated with biologic drugs in different time points of the treatment following the manufacturers´ instructions. We also analyzed the differences in histone methylation between patients from the dermatology unit and healthy subjects. It is ready to use, easy and fast to perform.
Other Thoughts
The amount of Antibody buffer (GD6) is quite restrictive. The kit does not include enough volume to perform pre-dilutions of the samples. Moreover, the strip wells do not bind properly to the plate. Therefore, it sometimes causes troubles when you introduce the plate in the Multiskan, causing it to get stuck. When we measured the background of the empty plates that had been at room temperature for at least 15 minutes, we saw that the background was not very homogenous. As the expected differences between our case and control samples were quite small, the variability found in the background may mask the specific differences within them. However, I think there must be difficult to reduce such variation. It is important to underlie that all materials needed are included in the kit. To carry out the experiments you only need a microplate spectrophotometer and a centrifuge.
Differences in the percentage of methylated H3K4 in peripheral blood mononuclear cells.
Differences in the percentage of methylated H3K4 in peripheral blood mononuclear cells between our cases and controls.
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