EpiQuik Global Histone H3K4 Methylation Assay Kit

The EpiQuik™ Global Histone H3-K4 Methylation Assay Kit is suitable for specifically measuring global histone H3-K4 methylation using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells. The kit has the following advantages:

• Quick and efficient procedure, which can be finished within 5 hours.
• Innovative colorimetric assay without the need for radioactivity, electrophoresis, and chromatography.
• Strip microwell format makes the assay flexible: manual or high throughput.
• Simple, reliable, and consistent assay conditions.

Principle & Procedure
The EpiQuik™ Global H3-K4 Methylation Assay Kit is designed for measuring global histone H3-K4 methylation. In an assay with this kit, the histone proteins are stably spotted on the strip wells. The methylated histone H3-K4 can be recognized with a high-affinity antibody The ratio or amount of methylated H3-K4 can be quantified through HRP conjugated secondary antibody-color development system and is proportional to the intensity of color development.