The EpiQuik™ Global Histone H3-K27 Methylation Assay Kit is suitable for specifically measuring global histone H3-K27 methylation using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells. The kit has the following advantages:
- Quick and efficient procedure, which can be finished within 5 hours.
- Innovative colorimetric assay without the need for radioactivity, electrophoresis, and chromatography.
- Strip microwell format makes the assay flexible: manual or high throughput.
- Simple, reliable, and consistent assay conditions.
Principle & Procedure
The EpiQuik™ Global H3-K27 Methylation Assay Kit is designed for measuring global histone H3-K27 methylation. In an assay with this kit, the histone proteins are stably spotted on the strip wells. The methylated histone H3-K27 can be recognized with a high-affinity antibody. The ratio or amount of methylated H3-K27 can be quantified through HRP conjugated secondary antibody-color development system and is proportional to the intensity of color development.