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   Home  »  Research Kits  »  Histone Methylation  »  Histone Methylation Quantification  »  EpiQuik Global Histone H3K27 Methylation Assay Kit 
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Base Cat #P-3020

EpiQuik Global Histone H3K27 Methylation Assay Kit

3.02 Reviews|15 Citations

For ELISA-like measurement of global histone H3K27 methylation

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Schematic procedure for using the EpiQuik Global Histone H3K27 Methylation Assay Kit.
Input Type: Cells, Unisolated Samples
Research Area: Histone Methylation
Target Application: Amount Quantitation
Vessel Format: 96-Well Plate
100% Guarantee: 6 months
Why EpiGentek AntibodiesApplication Guarantee for Antibodies
Catalog No.SizePriceQty
P-3020-4848 assays $470.00 
P-3020-9696 assays $699.00 
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Order now & get it by Tuesday, July 21st  

Product Overview

The EpiQuik™ Global Histone H3-K27 Methylation Assay Kit is suitable for specifically measuring global histone H3-K27 methylation using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells. The kit has the following advantages:

  • Quick and efficient procedure, which can be finished within 5 hours.
  • Innovative colorimetric assay without the need for radioactivity, electrophoresis, and chromatography.
  • Strip microwell format makes the assay flexible: manual or high throughput.
  • Simple, reliable, and consistent assay conditions.

Principle & Procedure
The EpiQuik™ Global H3-K27 Methylation Assay Kit is designed for measuring global histone H3-K27 methylation. In an assay with this kit, the histone proteins are stably spotted on the strip wells. The methylated histone H3-K27 can be recognized with a high-affinity antibody. The ratio or amount of methylated H3-K27 can be quantified through HRP conjugated secondary antibody-color development system and is proportional to the intensity of color development.

Datasheet & SDS

PDF
Datasheet/User Guide: P-3020.pdf If the above file is not available online, you can request a copy by contacting us.
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Customer Reviews

Write a Review
Rating ★★★☆☆3/5 via Distributor/Reseller Verified Purchase
Reviewed on: Tuesday 21 November, 2017
Application Description
We analyzed the histone modifications associated with biologic drugs in different time points of the treatment following the manufacturers´ instructions. ...
Rating ★★★☆☆3/5 via Distributor/Reseller Verified Purchase
Reviewed on: Friday 03 November, 2017
Application Description
We analyzed the histone modifications associated with biologic drugs in different time points of the treatment following the manufacturers´ instructions. ...
  1. Kumar P et. al. (August 2024). Epigenetic mechanisms differentially regulate blood pressure and renal dysfunction in male and female Npr1 haplotype mice. FASEB J. 38(15):e23858.
  2. Adams-Sherrod GA et. al. (July 2024). Sex-specific modulation of renal epigenetic and injury markers in aging kidney. Am J Physiol Renal Physiol.
  3. Bredel M et. al. (June 2023). Haploinsufficiency of NFKBIA reshapes the epigenome antipodal to the IDH mutation and imparts disease fate in diffuse gliomas. Cell Rep Med. 4(6):101082.
  4. Yoda M et. al. (April 2023). Nicotinamide-N-methyltransferase regulates lipid metabolism via SAM and 1-methylnicotinamide in the AML12 hepatocyte cell line. J Biochem.
  5. Zhao Z et. al. (April 2023). The putative methyltransferase LaeA regulates mycelium growth and cellulase production in Myceliophthora thermophila. Biotechnol Biofuels Bioprod. 16(1):58.
  6. Deniz BF et. al. (May 2021). High Gestational Folic Acid Supplementation Prevents Hypoxia-Ischemia-Induced Caspase-3 Augmenting Without Changing Synapsin And H3 Methylation Levels In The Rat Hippocampus. Int J Dev Neurosci.
  7. Shankar E et. al. (August 2020). Dual targeting of EZH2 and androgen receptor as a novel therapy for castration-resistant prostate cancer. Toxicol Appl Pharmacol. :115200.
  8. Mei X et. al. (July 2020). Fasciola gigantica excretory-secretory products (FgESPs) modulate the differentiation and immune functions of buffalo dendritic cells through a mechanism involving DNMT1 and TET1. Parasit Vectors. 13(1):355.
  9. Bilan Li et. al. (March 2017). DICER1 regulates endometrial carcinoma invasion via histone acetylation and methylation Journal of Cancer. 8:933 -939.
  10. Li B et. al. (March 2017). DICER1 regulates endometrial carcinoma invasion via histone acetylation and methylation. J Cancer. 8(6):933-939.
  11. Burgess SL et. al. (July 2016). Role of Serum Amyloid A, GM-CSF and Bone Marrow Granulocyte-Monocyte Precursor Expansion in Segmented Filamentous Bacteria-mediated Protection from Entamoeba histolytica. Infect Immun.
  12. Badisa RB et. al. (January 2015). N-acetyl cysteine mitigates the acute effects of cocaine-induced toxicity in astroglia-like cells. PLoS One. 10(1):e0114285.
  13. Vishnubalaji R et. al. (January 2015). Genome-wide mRNA and miRNA expression profiling reveal multiple regulatory networks in colorectal cancer. Cell Death Dis. 6:e1614.
  14. Batra V et. al. (March 2012). Interaction between γ-radiation and dietary folate starvation metabolically reprograms global hepatic histone H3 methylation at lysine 4 and lysine 27 residues. Food Chem Toxicol. 50(3-4):464-72.
  15. Chen C et. al. (October 2011). Abnormal histone acetylation and methylation levels in esophageal squamous cell carcinomas. Cancer Invest. 29(8):548-56.
Rating ★★★☆☆3/5 via Distributor/Reseller Verified Purchase
Reviewed on: Tuesday 21 November, 2017
Application Description
We analyzed the histone modifications associated with biologic drugs in different time points of the treatment following the manufacturers´ instructions. It is ready to use, easy and fast to perform.
Other Thoughts
The amount of Antibody buffer (GG6) is quite restrictive. The kit does not include enough volume to perform pre-dilutions of the samples. The strip wells do not bind properly to the plate. Therefore, it sometimes causes troubles when you introduce the plate in the Multiskan, causing it to get stuck. When we measured the background of the empty plates that had been at room temperature for at least 15 minutes, we saw that the background was not very homogenous. As the expected differences between our case and control samples were quite small, the variability found in the background may mask the specific differences within them. Nevertheless, I think there must be difficult to reduce such variation. It is important to underlie that all materials needed are included in the kit. To carry out the experiments you only need a microplate spectrophotometer and a centrifuge.
Differences in the percentage of methylated H3K27 in peripheral blood mononuclear cells of healthy subjects.
Differences in the percentage of methylated H3K27 in peripheral blood mononuclear cells.
Rating ★★★☆☆3/5 via Distributor/Reseller Verified Purchase
Reviewed on: Friday 03 November, 2017
Application Description
We analyzed the histone modifications associated with biologic drugs in different time points of the treatment following the manufacturers´ instructions. In total, we have already used 4 units of this kit (3020-96) this year. It is ready to use, easy and fast to perform. The user guide was clear and easy to use. The presentation of the product was good.
Other Thoughts
The amount of Antibody buffer (GG6) is quite restrictive. The kit does not include enough volume to perform pre-dilutions of the samples. The strip wells do not bind properly to the plate. Therefore, it sometimes causes troubles when you introduce the plate in the Multiskan, causing it to get stuck. When we measured the background of the empty plates that had been at room temperature for at least 15 minutes, we saw that the background was not very homogenous. As the expected differences between our case and control samples were quite small, the variability found in the background may mask the specific differences within them. Nevertheless, I think there must be difficult to reduce such variation.

It is important to underlie that all materials needed are included in the kit. To carry out the experiments you only need a microplate spectrophotometer and a centrifuge.
Differences in the percentage of methylated H3K27 in peripheral blood mononuclear cells of healthy subjects.
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