EpiQuik In Situ Histone H3K9 Methylation Assay Kit

The EpiQuik™ In Situ Histone H3-K9 Methylation Assay Kit is a convenient package of tools that allows the experimenter to specifically measure histone H3-K9 methylation in situ using cultured adherent cells. The kit has the following advantages:

• Quick and efficient procedure, which can be finished within 3 hours.
• Innovative colorimetric assay without the need for radioactivity, electrophoresis, and chromatography.
• Measurement of in situ histone H3-K9 methylation without the need to prepare cell lysates.
• Microplate format makes the assay to be suitable for high throughput analysis of agents that increase or inhibit H3-K9 methylation.
• Simple, reliable, and consistent assay conditions.

Principle & Procedure
EpiQuik™ In Situ Histone H3-K9 Methylation Assay Kit is a whole cell-based detection of methylated H3-K9. In this assay, adherent cells are cultured in conventional 96-well microplates. After your experimental treatment, cells are fixed and permeabilized. The methylated H3-K9 is then detected by a high affinity H3-K9 antibody. The ratio or amount of methylated H3-K9 can be quantified through HRP conjugated secondary antibody-color development system and is proportional to the intensity of color development.

 
Fig. 1. Schematic procedure for using the EpiQuik In Situ Histone H3K9 Methylation Assay Kit.