The EpiQuik™ In Situ Histone H3-K9 Methylation Assay Kit is a convenient package of tools that allows the experimenter to specifically measure histone H3-K9 methylation in situ using cultured adherent cells. The kit has the following advantages:
Principle & ProcedureEpiQuik™ In Situ Histone H3-K9 Methylation Assay Kit is a whole cell-based detection of methylated H3-K9. In this assay, adherent cells are cultured in conventional 96-well microplates. After your experimental treatment, cells are fixed and permeabilized. The methylated H3-K9 is then detected by a high affinity H3-K9 antibody. The ratio or amount of methylated H3-K9 can be quantified through HRP conjugated secondary antibody-color development system and is proportional to the intensity of color development.
Fig. 1. Schematic procedure for using the EpiQuik In Situ Histone H3K9 Methylation Assay Kit.