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EpiQuik In Situ Histone H3K9 Methylation Assay Kit

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Schematic procedure for using the EpiQuik In Situ Histone H3K9 Methylation Assay Kit.
Input Type: Cells
Research Area: Histone Methylation
Target Application: Amount Quantitation
Vessel Format: 96-Well Plate
100% Guarantee: 6 months
Catalog No.SizePriceQty
P-3016-09696 assays $399.00 
P-3016-1922x96 assays $658.00 
Availability: Usually Ships In 1-2 Days 
Product Overview

The EpiQuik™ In Situ Histone H3-K9 Methylation Assay Kit is a convenient package of tools that allows the experimenter to specifically measure histone H3-K9 methylation in situ using cultured adherent cells. The kit has the following advantages:

  • Quick and efficient procedure, which can be finished within 3 hours.
  • Innovative colorimetric assay without the need for radioactivity, electrophoresis, and chromatography.
  • Measurement of in situ histone H3-K9 methylation without the need to prepare cell lysates.
  • Microplate format makes the assay to be suitable for high throughput analysis of agents that increase or inhibit H3-K9 methylation.
  • Simple, reliable, and consistent assay conditions.

Principle & Procedure
EpiQuik™ In Situ Histone H3-K9 Methylation Assay Kit is a whole cell-based detection of methylated H3-K9. In this assay, adherent cells are cultured in conventional 96-well microplates. After your experimental treatment, cells are fixed and permeabilized. The methylated H3-K9 is then detected by a high affinity H3-K9 antibody. The ratio or amount of methylated H3-K9 can be quantified through HRP conjugated secondary antibody-color development system and is proportional to the intensity of color development.

 
Fig. 1. Schematic procedure for using the EpiQuik In Situ Histone H3K9 Methylation Assay Kit.

Product Components

GB1 (10X Wash Buffer)
GB2 (Permeabilizing Buffer)
GB3 (Blocking Buffer)
GB4 (Antibody Buffer)
GB5 (Capture Antibody, 100 µg/ml)*
GB6 (Detection Antibody, 400 µg/ml)*
GB7 (Developing Solution)
GB8 (Stop Solution)
30% H2O2 Solution
Methylated H3-K9 Control (20 µg/ml)
8-Well Control Strips
Microplates
User Guide

* For maximum recovery of the products, centrifuge the original vial prior to opening the cap.

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Material Safety Data Sheet]
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