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EpiQuik In Situ Histone H3K9 Methylation Assay Kit

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Schematic procedure for using the EpiQuik In Situ Histone H3K9 Methylation Assay Kit.
Input Type: Cells
Research Area: Histone Methylation
Target Application: Amount Quantitation
Vessel Format: 96-Well Plate
100% Guarantee: 6 months
Catalog No.SizePriceQty
P-3016-09696 assays $409.00 
P-3016-1922x96 assays $699.00 
Order now & get it by Thursday, November 14th  
Product Overview

The EpiQuik™ In Situ Histone H3-K9 Methylation Assay Kit is a convenient package of tools that allows the experimenter to specifically measure histone H3-K9 methylation in situ using cultured adherent cells. The kit has the following advantages:

  • Quick and efficient procedure, which can be finished within 3 hours.
  • Innovative colorimetric assay without the need for radioactivity, electrophoresis, and chromatography.
  • Measurement of in situ histone H3-K9 methylation without the need to prepare cell lysates.
  • Microplate format makes the assay to be suitable for high throughput analysis of agents that increase or inhibit H3-K9 methylation.
  • Simple, reliable, and consistent assay conditions.

Principle & Procedure
EpiQuik™ In Situ Histone H3-K9 Methylation Assay Kit is a whole cell-based detection of methylated H3-K9. In this assay, adherent cells are cultured in conventional 96-well microplates. After your experimental treatment, cells are fixed and permeabilized. The methylated H3-K9 is then detected by a high affinity H3-K9 antibody. The ratio or amount of methylated H3-K9 can be quantified through HRP conjugated secondary antibody-color development system and is proportional to the intensity of color development.

 
Fig. 1. Schematic procedure for using the EpiQuik In Situ Histone H3K9 Methylation Assay Kit.

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Safety Data Sheet]
BisulPlus Loci 5mC & 5hmC Detection PCR kit
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