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EpiQuik In Situ Histone H3K4 Methylation Assay Kit

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Schematic Procedure for Using the EpiQuikâ„¢ In Situ Histone H3K4 Methylation Assay Kit.
Input Type: Cells
Research Area: Histone Methylation
Target Application: Amount Quantitation
Vessel Format: 96-Well Plate
100% Guarantee: 6 months
Catalog No.SizePriceQty
P-3015-09696 assays $399.00 
P-3015-1922x96 assays $658.00 
Availability: Usually Ships In 1-2 Days 
Product Overview

The EpiQuik™ In Situ Histone H3K4 Methylation Assay Kit is a convenient package of tools that allows the experimenter to specifically measure histone H3K4 methylation in situ using cultured adherent cells. The kit has the following advantages:

  • Quick and efficient procedure, which can be finished within 3 hours
  • Innovative colorimetric assay without the need for radioactivity, electrophoresis, or chromatography
  • Measurement of in situ histone H3K4 methylation without the need to prepare cell lysates
  • Microplate format makes the assay suitable for high throughput analysis of agents that increases or inhibits H3-K4 methylation
  • Simple, reliable, and consistent assay conditions

Background 
Epigenetic activation or inactivation of genes play a critical role in many important human diseases, especially in cancer. A major mechanism for epigenetic inactivation of the genes is methylation of CpG islands in genome DNA caused by DNA methyltransferases. Histone methyltransferases (HMTs) control or regulate DNA methylation through chromatin-dependent transcription repression or activation. HMTs transfer 1-3 methyl groups from S-adenosyl-Lmethionine to the lysine and arginine residues of histone proteins. SET1, SET7/9, Ash1, ALL-1, MLL, ALR, Trx, and SMYD3 are histone methyltransferases that catalyze methylation of histone H3 at lysine 4 (H3-K4) in mammalian cells. H3-K4 methylation may serve as a global epigenetic mark in euchromatin and mediates activated transcription. Increased global H3-K4 methylation is also found to be involved in some pathological processes such as cancer progress. 

Principle & Procedure
The EpiQuik™ In Situ Histone H3K4 Methylation Assay Kit is a whole cell-based detection method for methylated H3-K4. In this assay, adherent cells are cultured in conventional 96-well microplates. After your experimental treatment, cells are fixed and permeabilized. The methylated H3K4 is then detected by an anti-methyl H3-K4 antibody. The ratio or amount of methylated H3K4 can be quantified through HRP conjugated secondary antibody-color development system and is proportional to the intensity of color development.

Product Components

GA1 (10X Wash Buffer)
GA2 (Permeabilizing Buffer)
GA3 (Blocking Buffer)
GA4 (Antibody Buffer)
GA5 (Capture Antibody, 100 µg/ml)*
GA6 (Detection Antibody, 200 µg/ml)*
GA7 (Developing Solution)
GA8 (Stop Solution)
30% H2O2 Solution
Methylated H3-K4 Control (20 µg/ml)
8-Well Control Strips
Microplates
User Guide

* For maximum recovery of the products, centrifuge the original vial prior to opening the cap.

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Material Safety Data Sheet]
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