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   Home  »  Research Kits  »  Histone Acetylation  »  Histone Acetylation Quantification  »  EpiQuik Global Histone H3 Acetylation Assay Kit 
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Base Cat #P-4008

EpiQuik Global Histone H3 Acetylation Assay Kit

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Suggested Workflow
Histone Protein Extraction
 
 
Histone H3 Acetylation Quantification
 
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Schematic procedure for using the EpiQuik Global Histone H3 Acetylation Assay Kit.
Acetylated histone H3 control was bound to microplate wells at different concentrations and measured using the EpiQuik™ Global Histone H3 Acetylation Assay Kit.
Input Type: Histone Extracts
Research Area: Histone Acetylation
Target Application: Amount Quantitation
Vessel Format: 96-Well Plate
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Catalog No.SizePriceQty
P-4008-4848 assays $443.00 
P-4008-9696 assays $630.00 
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Product Overview

The EpiQuik™ Global Histone H3 Acetylation Assay Kit is a convenient package of tools that allows the experimenter to measure global acetylation of histone H3 at tremendously fast speeds and consistency, superior and safer than all other current methods. The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment. The EpiQuik™ Global Histone H3 Acetylation Assay Kit is suitable for specifically measuring global histone H3 acetylation using a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells. The kit has the following advantages:

  • Quick and efficient procedure, which can be finished within 5 hours.
  • Innovative colorimetric assay without the need for radioactivity, electrophoresis, or chromatography.
  • Strip microplate format makes the assay flexible: manual or high throughput analysis.
  • Simple, reliable, and consistent assay conditions.

Principle & Procedure
The EpiQuik™ Global Histone H3 Acetylation Assay Kit is designed for measuring global histone H3 acetylation. In an assay with this kit, the histone proteins are stably spotted on the strip wells. The acetylated histone H3 can be recognized with a high-affinity antibody. The ratio or amount of acetylated histone H3 can be quantified through HRP conjugated secondary antibody-color development system and is proportional to the intensity of color development.

Datasheet & SDS

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Datasheet/User Guide: P-4008.pdf If the above file is not available online, you can request a copy by contacting us.
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  1. Koc S et. al. (January 2025). Prepubertal phthalate exposure can cause histopathological alterations, DNA methylation and histone acetylation changes in rat brain. Toxicol Ind Health. :7482337251315212.
  2. Jeffrey MP et. al. (April 2024). A Lacticaseibacillus rhamnosus secretome induces immunoregulatory transcriptional, functional and immunometabolic signatures in human THP-1 monocytes. Sci Rep. 14(1):8379.
  3. Qin Y et. al. (January 2024). Neurotoxic effects induced by flunitrazepam and its metabolites in zebrafish: Oxidative stress, apoptosis, and histone hypoacetylation. Sci Total Environ. :170521.
  4. Balci-Ozyurt A et. al. (October 2023). Evaluation of possible cytotoxic, genotoxic and epigenotoxic effects of titanium dioxide nanoparticles and possible protective effect of melatonin. Toxicol Mech Methods. :1-13.
  5. Convertini P et. al. (August 2023). ACLY as a modulator of liver cell functions and its role in Metabolic Dysfunction-Associated Steatohepatitis. J Transl Med. 21(1):568.
  6. Reyes-Mata MP et. al. (May 2023). Global DNA Methylation and Hydroxymethylation Levels in PBMCs Are Altered in RRMS Patients Treated with IFN-β and GA-A Preliminary Study. Int J Mol Sci. 24(10)
  7. Knoche SM et. al. (September 2022). The histone deacetylase inhibitor M344 as a multifaceted therapy for pancreatic cancer. PLoS One. 17(9):e0273518.
  8. Roy S. et. al. (May 2022). Induction of transgenerational innate immune memory against Vibrio infections in a brine shrimp (Artemia franciscana) model Aquaculture. 557
  9. Li Z et. al. (April 2022). Histone acetyltransferase GCN5-mediated lysine acetylation modulates salt stress aadaption of Trichoderma. Appl Microbiol Biotechnol. 106(8):3033-3049.
  10. Roig-Soriano J et. al. (March 2022). AAV-mediated expression of secreted and transmembrane αKlotho isoforms rescues relevant aging hallmarks in senescent SAMP8 mice. Aging Cell. :e13581.
  11. Kunhorm P et. al. (March 2022). Enhancement of cordycepin production from Cordyceps militaris culture by epigenetic modification. Biotechnol Lett.
  12. Fraga I et. al. (December 2021). Effects of a multimodal exercise protocol on functional outcomes, epigenetic modulation and brain-derived neurotrophic factor levels in institutionalized older adults: a quasi-experimental pilot study. Neural Regen Res. 16(12):2479-2485.
  13. Ehteda A et. al. (April 2021). Dual targeting of the epigenome via FACT complex and histone deacetylase is a potent treatment strategy for DIPG. Cell Rep. 35(2):108994.
  14. Mak MP et. al. (December 2020). Valproic acid combined with cisplatin-based chemoradiation in locally advanced head and neck squamous cell carcinoma patients and associated biomarkers. Ecancermedicalscience. 14:1155.
  15. Passamonti M et. al. (May 2020). Clues of in vivo nuclear gene regulation by mitochondrial short non-coding RNAs. Sci Rep. 10(1):8219.
  16. Xie Y et. al. (May 2020). LINC01198 facilitates gliomagenesis through activating PI3K/AKT pathway. RNA Biol. :1-13.
  17. da Costa Loureiro L et. al. (April 2020). Pulmonary surfactant phosphatidylcholines induce immunological adaptation of alveolar macrophages. Mol Immunol. 122:163-172.
  18. Sun L et. al. (April 2020). Activation of Tyrosine Metabolism in CD13+ Cancer Stem Cells Drives Relapse in Hepatocellular Carcinoma. Cancer Res Treat. 52(2):604-621.
  19. Masui K et. al. (November 2019). mTORC2 links growth factor signaling with epigenetic regulation of iron metabolism in glioblastoma. J Biol Chem.
  20. Giacomet C et. al. (August 2019). Does a HIIT modulate BDNF levels, and epigenetic and muscle damage markers in postmenopausal obese women? Comparative Exercise Physiology. :1-10.
  21. Wang Y et. al. (June 2019). Aquaporin 3 maintains the stemness of CD133+ hepatocellular carcinoma cells by activating STAT3. Cell Death Dis. 10(6):465.
  22. Sun N et. al. (May 2019). Effects of histone deacetylase inhibitor Scriptaid and Parathyroid Hormone on osteocyte functions and metabolism. J Biol Chem.
  23. Ovejero-Benito MC et. al. (September 2018). Histone modifications associated with biological drug response in moderate-to-severe psoriasis. Exp Dermatol.
  24. Yin M et. al. (January 2018). Micro Integral Membrane Protein (MIMP), a Newly Discovered Anti-Inflammatory Protein of Lactobacillus Plantarum, Enhances the Gut Barrier and Modulates Microbiota and Inflammatory Cytokines. Cell Physiol Biochem. 45(2):474-490.
  25. Li R et. al. (December 2017). Serum CCL20 and its association with SIRT1 activity in multiple sclerosis patients. J Neuroimmunol. 313:56-60.
  26. Ding S et. al. (November 2017). Chronic sun exposure is associated with distinct histone acetylation changes in human skin. Br J Dermatol.
  27. Bilan Li et. al. (March 2017). DICER1 regulates endometrial carcinoma invasion via histone acetylation and methylation Journal of Cancer. 8:933 -939.
  28. Zhou K et. al. (February 2017). Aberrant histone modification in CD19<sup>+</sup> B cells of patients with chronic lymphocytic leukemia. Onco Targets Ther. 10:1173-1179.
  29. Bramble MS et. al. (November 2016). Sex-Specific Effects of Testosterone on the Sexually Dimorphic Transcriptome and Epigenome of Embryonic Neural Stem/Progenitor Cells. Sci Rep. 6:36916.
  30. Li Y et. al. (August 2016). Combinatorial epigenetic mechanisms and efficacy of early breast cancer inhibition by nutritive botanicals. Epigenomics. 8(8):1019-37.
  31. Sramek M et. al. (February 2016). Non-DHFR-mediated effects of methotrexate in osteosarcoma cell lines: epigenetic alterations and enhanced cell differentiation. Cancer Cell Int. 16:14.
  32. Sharma S et. al. (January 2016). Epigenetic modifications by inhibiting histone deacetylases reverse memory impairment in insulin resistance induced cognitive deficit in mice. Neuropharmacology. 105:285-297.
  33. Liu XY et. al. (December 2015). Reduced Histone H3 Acetylation in CD4(+) T Lymphocytes: Potential Mechanism of Latent Autoimmune Diabetes in Adults. Dis Markers. 2015:285125.
  34. Xi-yu Liu and Jiang-feng Xu et. al. (December 2015). Reduced Histone H3 Acetylation in CD4+ T Lymphocytes: Potential Mechanism of Latent Autoimmune Diabetes in Adults Disease Markers.
  35. Yan N et. al. (June 2015). Histone hypoacetylation and increased histone deacetylases in peripheral blood mononuclear cells from patients with Graves' disease. Mol Cell Endocrinol.
  36. Novohradsky V et. al. (June 2015). New insights into the molecular and epigenetic effects of antitumor Pt(IV)-valproic acid conjugates in human ovarian cancer cells. Biochem Pharmacol. 95(3):133-44.
  37. Yang Y et. al. (March 2015). The effect of mycophenolic acid on epigenetic modifications in lupus CD4<sup>+</sup>T cells. Clin Immunol.
  38. Sutendra G et. al. (July 2014). A nuclear pyruvate dehydrogenase complex is important for the generation of acetyl-CoA and histone acetylation. Cell. 158(1):84-97.
  39. Kumar P et. al. (June 2014). All-trans retinoic acid and sodium butyrate enhance natriuretic peptide receptor a gene transcription: role of histone modification. Mol Pharmacol. 85(6):946-57.
  40. Kumar P et. al. (March 2014). Histone deacetylase inhibitors modulate the transcriptional regulation of guanylyl cyclase/natriuretic peptide receptor-a gene: interactive roles of modified histones, histone acetyltransferase, p300, AND Sp1. J Biol Chem. 289(10):6991-7002.
  41. Cho Y et. al. (March 2014). Colon cancer cell apoptosis is induced by combined exposure to the n-3 fatty acid docosahexaenoic acid and butyrate through promoter methylation. Exp Biol Med (Maywood). 239(3):302-10.
  42. Monteiro JB et. al. (March 2014). Endometriosis is characterized by a distinct pattern of histone 3 and histone 4 lysine modifications. Reprod Sci. 21(3):305-18.
  43. Habib O et. al. (December 2013). An improved method for the derivation of high quality iPSCs in the absence of c-Myc. Exp Cell Res. 319(20):3190-200.
  44. Doherty R et. al. (August 2013). Epigenetic regulation of the innate immune response to LPS in bovine peripheral blood mononuclear cells (PBMC). Vet Immunol Immunopathol. 154(3-4):102-10.
  45. Xiaomeng X et. al. (March 2013). Aberrant histone acetylation and methylation levels in woman with endometriosis. Arch Gynecol Obstet. 287(3):487-94.
  46. Rehan VK et. al. (October 2012). Perinatal nicotine exposure induces asthma in second generation offspring. BMC Med. 10:129.
  47. Ghadimi D et. al. (October 2012). Epigenetic imprinting by commensal probiotics inhibits the IL-23/IL-17 axis in an in vitro model of the intestinal mucosal immune system. J Leukoc Biol. 92(4):895-911.
  48. Easley CA 4th et. al. (June 2012). Human amniotic epithelial cells are reprogrammed more efficiently by induced pluripotency than adult fibroblasts. Cell Reprogram. 14(3):193-203.
  49. Zhao M et. al. (February 2012). Abnormal epigenetic modifications in peripheral blood mononuclear cells from patients with alopecia areata. Br J Dermatol. 166(2):226-73.
  50. Sanchez-Roman I et. al. (December 2011). Forty percent methionine restriction lowers DNA methylation, complex I ROS generation, and oxidative damage to mtDNA and mitochondrial proteins in rat heart. J Bioenerg Biomembr. 43(6):699-708.
  51. Zhang Z et. al. (October 2011). Epigenetic suppression of GAD65 expression mediates persistent pain. Nat Med. 17(11):1448-55.
  52. Chen C et. al. (October 2011). Abnormal histone acetylation and methylation levels in esophageal squamous cell carcinomas. Cancer Invest. 29(8):548-56.
  53. Kraft M et. al. (September 2011). Disruption of the histone acetyltransferase MYST4 leads to a Noonan syndrome-like phenotype and hyperactivated MAPK signaling in humans and mice. J Clin Invest. 121(9):3479-91.
  54. Rivière G et. al. (April 2011). Epigenetic regulation of somatic angiotensin-converting enzyme by DNA methylation and histone acetylation. Epigenetics. 6(4):478-89.
  55. Sui L et. al. (December 2010). Effects of perinatal hypothyroidism on regulation of reelin and brain-derived neurotrophic factor gene expression in rat hippocampus: Role of DNA methylation and histone acetylation. Steroids. 75(12):988-97.
  56. Kobori A et. al. (May 2010). Butyrate stimulates IL-32alpha expression in human intestinal epithelial cell lines. World J Gastroenterol. 16(19):2355-61.
  57. Nicolai J et. al. (April 2010). CXCL12 inhibits expression of the NMDA receptor's NR2B subunit through a histone deacetylase-dependent pathway contributing to neuronal survival. Cell Death Dis. 1:e33.
  58. Biermann J et. al. (January 2010). Valproic acid-mediated neuroprotection and regeneration in injured retinal ganglion cells. Invest Ophthalmol Vis Sci. 51(1):526-34.
  59. Hu N et. al. (November 2009). Aberrant expression pattern of histone acetylation modifiers and mitigation of lupus by SIRT1-siRNA in MRL/lpr mice. Scand J Rheumatol. 38(6):464-71.
  60. Hu N et. al. (May 2008). Abnormal histone modification patterns in lupus CD4+ T cells. J Rheumatol. 35(5):804-10.
  61. Tsuchiya N et. al. (December 2004). Association of a functional CD19 polymorphism with susceptibility to systemic sclerosis. Arthritis Rheum. 50(12):4002-7.
  62. Bartra C et. al. Activation of anti-inflammatory pathways by polyunsaturated fatty acid signaling may protect neurodevelopment in children prenatally exposed to methylmercury. Environ Health. 25(1) PubMed: 41987264

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