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EpiQuik In Situ Histone H3K27 Tri-Methylation Assay Kit

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Schematic procedure for using the EpiQuik In Situ Histone H3K27 Tri-Methylation Assay Kit.
MDA-231 cancer cells were grown in microplate wells for 48 h and tri-methyl histone H3-K27 was detected with this kit.
Input Type: Cells
Research Area: Histone Methylation
Target Application: Amount Quantitation
Vessel Format: 96-Well Plate
100% Guarantee: 6 months
Catalog No.SizePriceQty
P-3014T-09696 assays $399.00 
P-3014T-1922x96 assays $658.00 
Availability: Usually Ships In 1-2 Days 
Product Overview

The EpiQuik™ In Situ Histone H3-K27 Tri-Methylation Assay Kit is a convenient package of tools that allows the experimenter to specifically measure histone H3-K27 tri-methylation in situ using cultured adherent cells. The kit has the following advantages:

  • Quick and efficient procedure, which can be finished within 3 hours.
  • Innovative colorimetric assay without the need for radioactivity, electrophoresis, and chromatography.
  • Measurement of in situ histone H3-K27 tri-methylation without the need to prepare cell lysates.
  • Microplate format makes the assay suitable for high throughput analysis of agents that increases or inhibits H3-K27 tri-methylation.
  • Simple, reliable, and consistent assay conditions.

Principle & Procedure
EpiQuik™ In Situ Histone H3-K27 Tri-Methylation Assay Kit is a whole cell-based detection of tri-methylated H3-K27. In this assay, adherent cells are cultured in conventional 96-well microplates. After your experimental treatment, cells are fixed and permeabilized. The tri- methylated H3-K27 is then detected by an anti-tri-methyl H3-K27 antibody. The ratio or amount of tri-methylated H3 -K27 can be quantified through HRP conjugated secondary antibody-color development system and is proportional to the intensity of color development.

 
Fig. 1. Schematic procedure for using the EpiQuik In Situ Histone H3K27 Tri-Methylation Assay Kit.

Fig. 2. MDA-231 cancer cells were grown in microplate wells for 48 h and tri-methyl histone H3-K27 was detected with this kit.

Product Components

GK1 (10X Wash Buffer)
GK2 (Permeabilizing Buffer)
GK3 (Blocking Buffer)
GK4 (Antibody Buffer)
GK5 (Capture Antibody, 1000 µg/ml)*
GK6 (Detection Antibody, 200 µg/ml)*
GK7 (Developing Solution)
GK8 (Stop Solution)
30% H2O2 Solution
Tri-Methyl H3-K27 Control (20 µg/ml)
8-Well Control Strips
Microplate
User Guide

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

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