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EpiQuik Global Histone H3K9 Methylation Assay Kit

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Schematic procedure for using the EpiQuik Global Histone H3K9 Methylation Assay Kit.
Measurement of H3-K9 methylation with different concentrations of the di-methyl H3-K9 standard from the EpiQuikâ„¢ Global Histone H3-K9 Methylation Assay Kit.
Input Type: Cells, Unisolated Samples
Research Area: Histone Methylation
Target Application: Amount Quantitation
Vessel Format: 96-Well Plate
100% Guarantee: 6 months
Catalog No.SizePriceQty
P-3018-4848 assays $369.00 
P-3018-9696 assays $549.00 
Availability: Usually Ships In 1-2 Days 
Product Overview

The EpiQuik™ Global Histone H3-K9 Methylation Assay Kit is suitable for specifically measuring global histone H3-K9 methylation using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells. The kit has the following advantages:

  • Quick and efficient procedure, which can be finished within 5 hours.
  • Innovative colorimetric assay without the need for radioactivity, electrophoresis, and chromatography.
  • Strip microwell format makes the assay flexible: manual or high throughput.
  • Simple, reliable, and consistent assay conditions.

Principle & Procedure
The EpiQuik™ Global H3-K9 Methylation Assay Kit is designed for measuring global histone H3-K9 methylation. In an assay with this kit, the histone proteins are stably spotted on the strip wells. The methylated histone H3-K9 can be recognized with a high-affinity antibody. The ratio or amount of methylated H3-K9 can be quantified through HRP conjugated secondary antibody-color development system and is proportional to the intensity of color development.

 
Fig. 1. Schematic procedure for using the EpiQuik Global Histone H3K9 Methylation Assay Kit.

Fig. 2. Measurement of H3-K9 methylation with different concentrations of the di-methyl H3-K9 standard from the EpiQuik™ Global Histone H3-K9 Methylation Assay Kit.

Product Components

GC1 (10X Lysis Buffer)
GC2 (Extraction Buffer)
GC3 (10X Wash Buffer)
GC4 (Histone Buffer)
GC5 (Blocking Buffer)
GC6 (Antibody Buffer)
GC7 (Capture Antibody, 100 µg/ml)*
GC8 (Detection Antibody, 400 µg/ml)*
GC9 (Developing Solution)
GC10 (Stop Solution)
Methylated H3-K9 Control (60 µg/ml)*
8-Well Assay Strips (with Frame)
User Guide

* For maximum recovery of the products, centrifuge the original vial prior to opening the cap.

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Material Safety Data Sheet]
Product Citations

Berenguer E et. al. (June 2017). Inhibition of Histone H3K9 Methylation by BIX-01294 Promotes Stress-Induced Microspore Totipotency and Enhances Embryogenesis Initiation. Front Plant Sci. 8:1161.

Ding R et. al. (March 2017). Dose- and time- effect responses of DNA methylation and histone H3K9 acetylation changes induced by traffic-related air pollution. Sci Rep. 7:43737.

Zhou K et. al. (February 2017). Aberrant histone modification in CD19<sup>+</sup> B cells of patients with chronic lymphocytic leukemia. Onco Targets Ther. 10:1173-1179.

Yang Y et. al. (March 2015). The effect of mycophenolic acid on epigenetic modifications in lupus CD4<sup>+</sup>T cells. Clin Immunol.

Kumar P et. al. (June 2014). All-trans retinoic acid and sodium butyrate enhance natriuretic peptide receptor a gene transcription: role of histone modification. Mol Pharmacol. 85(6):946-57.

Zhao H et. al. (January 2014). Aberrant histone methylation in the patients with immune thrombocytopenia. Platelets. 25(3):207-10.

Xiaomeng X et. al. (March 2013). Aberrant histone acetylation and methylation levels in woman with endometriosis. Arch Gynecol Obstet. 287(3):487-94.

Zhao M et. al. (February 2012). Abnormal epigenetic modifications in peripheral blood mononuclear cells from patients with alopecia areata. Br J Dermatol. 166(2):226-73.

Hu N et. al. (May 2008). Abnormal histone modification patterns in lupus CD4+ T cells. J Rheumatol. 35(5):804-10.

Tsuchiya N et. al. (December 2004). Association of a functional CD19 polymorphism with susceptibility to systemic sclerosis. Arthritis Rheum. 50(12):4002-7.

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