The EpiQuik™ Global Histone H3-K9 Methylation Assay Kit is suitable for specifically measuring global histone H3-K9 methylation using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells. The kit has the following advantages:
Principle & ProcedureThe EpiQuik™ Global H3-K9 Methylation Assay Kit is designed for measuring global histone H3-K9 methylation. In an assay with this kit, the histone proteins are stably spotted on the strip wells. The methylated histone H3-K9 can be recognized with a high-affinity antibody. The ratio or amount of methylated H3-K9 can be quantified through HRP conjugated secondary antibody-color development system and is proportional to the intensity of color development.
Fig. 1. Schematic procedure for using the EpiQuik Global Histone H3K9 Methylation Assay Kit.
Fig. 2. Measurement of H3-K9 methylation with different concentrations of the di-methyl H3-K9 standard from the EpiQuik™ Global Histone H3-K9 Methylation Assay Kit.
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