Save $100 on Antibodies Save $100 when you buy $500 in antibodies. Use code E1705ABR. Expires June 30th. » Learn More
Epigentek Home
 
Epigentek Free Shipping

EpiQuik Global Histone H3K27 Tri-Methylation Assay Kit

Share:

Citations (1) | Write a Review
Schematic procedure for using the EpiQuik Global Histone H3K27 Tri-Methylation Assay Kit.
Input Type: Cells, Unisolated Samples
Research Area: Histone Methylation
Target Application: Amount Quantitation
Vessel Format: 96-Well Plate
100% Guarantee: 6 months
Catalog No.SizePriceQty
P-3020T-4848 assays $369.00 
P-3020T-9696 assays $549.00 
Availability: Usually Ships In 1-2 Days 
Product Overview

The EpiQuik™ Global Histone H3-K27 Tri-Methylation Assay Kit is suitable for specifically measuring global histone H3-K27 tri-methylation using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells. The kit has the following advantages:

  • Quick and efficient procedure, which can be finished within 5 hours.
  • Innovative colorimetric assay without the need for radioactivity, electrophoresis, or chromatography.
  • Strip microwell format makes the assay flexible: manual or high throughput.
  • Simple, reliable, and consistent assay conditions.

Principle & Procedure
The EpiQuik™ Global H3-K27 Tri-Methylation Assay Kit is designed for measuring global histone H3-K27 tri-methylation. In an assay with this kit, the histone proteins are stably spotted on the strip wells. The tri-methylated histone H3-K27 can be recognized with a high-affinity antibody. The ratio or amount of tri-methylated H3-K27 can be quantified through HRP conjugated secondary antibody-color development system and is proportional to the intensity of color development.

 
Fig. 1. Schematic procedure for using the EpiQuik Global Histone H3K27 Tri-Methylation Assay Kit.

Product Components

GT1 (10X Lysis Buffer)
GT2 (Extraction Buffer)
GT3 (10X Wash Buffer)
GT4 (Histone Buffer)
GT5 (Blocking Buffer)
GT6 (Antibody Buffer)
GT7 (Capture Antibody, 100 µg/ml)*
GT8 (Detection Antibody, 400 µg/ml)*
GT9 (Developing Solution)
GT10 (Stop Solution)
Tri-Methyl H3-K27 Control (60 µg/ml)*
8-Well Assay Strips (with frame)
User Guide

* For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap.

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

Product Citations

Deligezer U et. al. (October 2010). Post-treatment circulating plasma BMP6 mRNA and H3K27 methylation levels discriminate metastatic prostate cancer from localized disease. Clin Chim Acta. 411(19-20):1452-6.

Replace Your Santa Cruz Abs

Researchers Also Purchased
EpiQuik Global Histone H3 Acetylation Assay Kit
EpiQuik Global Histone H3 Acetylation Assay Kit
EpiQuik Global Di-Methyl Histone H3K9 Quantification Kit (Colorimetric)
EpiQuik Global Di-Methyl Histone H3K9 Quantification Kit (Colorimetric)
MethylFlash Hydroxymethylated DNA 5-hmC Quantification Kit (Colorimetric)
MethylFlash Hydroxymethylated DNA 5-hmC Quantification Kit (Colorimetric)
EpiQuik Global Histone H3K27 Methylation Assay Kit
EpiQuik Global Histone H3K27 Methylation Assay Kit
Epigentek is an epigenetics company that specializes in epigenetic kits, antibodies, reagents, and services for epigenetic research in DNA methylation, histone modification, and chromatin studies. Terms & Conditions | Privacy Policy | Site Map
Copyright © 2017 Epigentek Group Inc. All rights reserved.