The EpiQuik™ Global Histone H3-K27 Tri-Methylation Assay Kit is suitable for specifically measuring global histone H3-K27 tri-methylation using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells. The kit has the following advantages:
Principle & ProcedureThe EpiQuik™ Global H3-K27 Tri-Methylation Assay Kit is designed for measuring global histone H3-K27 tri-methylation. In an assay with this kit, the histone proteins are stably spotted on the strip wells. The tri-methylated histone H3-K27 can be recognized with a high-affinity antibody. The ratio or amount of tri-methylated H3-K27 can be quantified through HRP conjugated secondary antibody-color development system and is proportional to the intensity of color development.
Fig. 1. Schematic procedure for using the EpiQuik Global Histone H3K27 Tri-Methylation Assay Kit.
Deligezer U et. al. (October 2010). Post-treatment circulating plasma BMP6 mRNA and H3K27 methylation levels discriminate metastatic prostate cancer from localized disease. Clin Chim Acta. 411(19-20):1452-6.