EpiQuik Global Histone H3K27 Tri-Methylation Assay Kit

We recommend using a newer version of this product: EpiQuik Global Tri-Methyl Histone H3K27 Quantification Kit (Colorimetric).

The EpiQuik™ Global Histone H3-K27 Tri-Methylation Assay Kit is suitable for specifically measuring global histone H3-K27 tri-methylation using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells. The kit has the following advantages:

• Quick and efficient procedure, which can be finished within 5 hours.
• Innovative colorimetric assay without the need for radioactivity, electrophoresis, or chromatography.
• Strip microwell format makes the assay flexible: manual or high throughput.
• Simple, reliable, and consistent assay conditions.

Principle & Procedure
The EpiQuik™ Global H3-K27 Tri-Methylation Assay Kit is designed for measuring global histone H3-K27 tri-methylation. In an assay with this kit, the histone proteins are stably spotted on the strip wells. The tri-methylated histone H3-K27 can be recognized with a high-affinity antibody. The ratio or amount of tri-methylated H3-K27 can be quantified through HRP conjugated secondary antibody-color development system and is proportional to the intensity of color development.

1. Shankar E et. al. (August 2020). Dual targeting of EZH2 and androgen receptor as a novel therapy for castration-resistant prostate cancer. Toxicol Appl Pharmacol. :115200.
2. Deligezer U et. al. (October 2010). Post-treatment circulating plasma BMP6 mRNA and H3K27 methylation levels discriminate metastatic prostate cancer from localized disease. Clin Chim Acta. 411(19-20):1452-6.