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EpiQuik Tissue Methyl-CpG Binding Domain Protein 2 ChIP Kit

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Suggested Workflow
Chromatin Isolation
 
 
Chromatin Shearing
 
 
MDB Protein ChIP
 
 
PCR Analysis
 
Schematic procedure for using the EpiQuik Tissue Methyl-CpG Binding Domain Protein 2 ChIP Kit.
Chromatin was precipitated from AN3CA cell line with the EpiQuikâ„¢ Tissue Methyl-CpG Binding Domain Protein 2 ChIP Kit. Real time PCR was used to amplify the promoter region of methylated P16.
Input Type: Chromatin
Research Area: Chromatin & Transcription
Target Application: Immunoprecipitation
Vessel Format: 96-Well Plate
100% Guarantee: 6 months
Catalog No.SizePriceQty
P-2018-2424 reactions $380.00 
P-2018-4848 reactions $549.00 
Availability: Usually Ships In 1-2 Days 
Product Overview

The EpiQuik™ Tissue Methyl-CpG Binding Domain Protein 2 ChIP Kit is a convenient package of tools that allows the experimenter to investigate the in vivo binding of MBD2 to methylated DNA in a variety of mammalian tissues. The entire procedure can be completed within 5 hours and produces far superior results than any competitor kits. The EpiQuik™ Tissue Methyl-CpG Binding Domain Protein 2 ChIP Kit is suitable for combining the specificity of immunoprecipitation with qualitative and quantitative PCR, MS-PCR, DNA sequencing, and southern blot as well as DNA microarray. The kit has the following advantages:

  • The fastest procedure available, which can be finished within 5 hours.
  • Strip microwell format makes the assay flexible: manual or high throughput.
  • Columns for DNA purification are included: save time and reduce labor.
  • Compatible with all DNA amplification-based approaches.
  • Simple, reliable, and consistent assay conditions.

Principle & Procedure
The EpiQuik™ Tissue Methyl-CpG Binding Domain Protein 2 ChIP Kit contains all reagents required for carrying out a successful Chromatin Immunoprecipitation for MBD2 from mammalian tissues. Particularly, this kit includes a ChIP-grade MBD2 antibody and a negative control normal mouse IgG. Chromatin from the cells is extracted, sheared, and added into the microwell immobilized with the antibody. DNA is released from the antibody-captured MBD2 protein-DNA complex, reversed and purified through the specifically designed Fast-Spin Column. Eluted DNA can be used for various down-stream applications.

Product Components

CP1 (Wash Buffer)
CP2 (Antibody Buffer)
CP3 (Lysis Buffer)
CP4 (ChIP Dilution Buffer)
CP5 (DNA Release Buffer)
CP6 (Reverse Buffer)
CP7 (Binding Buffer)
CP8 (Elution Buffer)
Homogenizing Buffer
Protease Inhibitor Cocktail (100X)*
Proteinase K (10 mg/ml)*
Normal Mouse IgG (0.5 mg/ml)*
Anti-MBD2 (0.5 mg/ml)*
8-Well Assay Strips (with Frame)
8-Well Strip Caps
F-Spin Column
F-Collection Tube
User Guide

* For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap.

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Material Safety Data Sheet]
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