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EpiQuik Acetyl-Histone H3 ChIP Kit

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For immunoprecipitating H3ac chromatin from cell input samples via microplate format

Citations (13) | (1) | Write a Review
Schematic procedure of the EpiQuik™ Acetyl-Histone H3 ChIP Kit.
Input Type: Chromatin
Research Area: Chromatin & Transcription
Target Application: Immunoprecipitation
Vessel Format: 96-Well Plate
100% Guarantee: 6 months
Catalog No.SizePriceQty
P-2010-2424 reactions $338.00 
P-2010-4848 reactions $539.00 
Availability: Usually Ships In 1-2 Days 
Product Overview

The EpiQuik™ Acetyl-Histone H3 ChIP Kit is a convenient package of tools that allows the experimenter to investigate interactions of histone H3 acetylation and DNA in vivo efficiently. The entire procedure can be completed within 5 hours and produces far superior results than any competitor kits. The EpiQuik™ Acetyl-Histone H3 ChIP Kit is suitable for combining the specificity of immunoprecipitation with qualitative and quantitative PCR, ChIP-Seq, and ChIP-on-chip. This kit has the following advantages: 

  • The fastest procedure available, which can be finished within 5 hours.
  • Strip microwell format makes the assay flexible: manual or high throughput.
  • Columns for DNA purification are included: save time and reduce labor.
  • Compatible with all DNA amplification-based approaches.
  • Simple, reliable, and consistent assay conditions.

Background Information
Histone acetylation takes place at the α-amino groups of conserved lysine residues, where histone acetyltransferases (HATs) are responsible for catalyzing this modification. It has also been demonstrated that the acetylation of histone H3 serves as an epigenetic marker of chromosomal domains. Histone H3 acetylation regulates various cellular physiological processes, including transcriptional activation of genes, chromatin assembly, cell proliferation, and some pathological processes such as tumorigenesis. Chromatin Immunoprecipitation (ChIP) is a powerful technique for studying protein-DNA interaction in vivo. ChIP also offers an advantageous tool that allows identification of activated genes associated with acetylated histone H3. ChIP coupled with microarrays could be used further for profiling or mapping histone H3 acetylation patterns.

Principle & Procedure
This ChIP kit contains all reagents required for carrying out a successful chromatin immunoprecipitation for acetyl-histone H3 from mammalian cells. Particularly, this kit includes a ChIP-grade acetyl-histone H3 antibody and a negative control normal mouse IgG. Chromatin in the cells is extracted, sheared, and added into the microwell immobilized with the antibody. Acetyl-histone H3 protein-DNA complex is captured by the antibody and DNA is released and purified through the specifically designed F-Spin Column. Eluted DNA can be used for various down-stream applications.  

Starting Materials
Starting materials can include various cell samples. In general, the input amount should be from 1,000,000 to 4,000,000 cells for each reaction.

Product Components

CP1 (Wash Buffer)
CP2 (Antibody Buffer)
CP3A (Pre-Lysis Buffer)
CP3B (Lysis Buffer)
CP4 (ChIP Dilution Buffer)
CP5 (DNA Release Buffer)
CP6 (Reverse Buffer)
CP7 (Binding Buffer)
CP8 (Elution Buffer)
Protease Inhibitor Cocktail (100X)*
Proteinase K (10 mg/ml)*
Normal Mouse IgG (0.5 mg/ml)*
Anti-Acetyl-Histone H3 (1 mg/ml)*
8-Well Assay Strips (with Frame)
8-Well Strip Caps
F-Spin Column
F-Collection Tube
User Guide

* For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap.

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Material Safety Data Sheet]
Product Citations

Qiao Liu et. al. (May 2017). Epigenetic down regulation of G protein-coupled estrogen receptor (GPER) functions as a tumor suppressor in colorectal cancer Molecular Cancer. 16(87)

Scholpa NE et. al. (August 2016). Nephrotoxicity of epigenetic inhibitors used for the treatment of cancer. Chem Biol Interact. 258:21-29.

Chen J et. al. (June 2016). Aberrant Epigenetic Alterations of Glutathione-S-Transferase P1 in Age-Related Nuclear Cataract. Curr Eye Res. :1-9.

Cheng W et. al. (May 2016). Mainstream smoke and sidestream smoke affect the cardiac differentiation of mouse embryonic stem cells discriminately. Toxicology.

Chen YP et. al. (March 2016). DNA methylation and histone acetylation regulate the expression of MGMT and chemosensitivity to temozolomide in malignant melanoma cell lines. Tumour Biol.

Etiévant A et. al. (November 2015). Repetitive transcranial magnetic stimulation induces long-lasting changes in protein expression and histone acetylation Sci Rep. 5

Zhu X et. al. (March 2015). Epigenetic regulation of Werner syndrome gene in age-related cataract. J Ophthalmology.

Tao W et. al. (June 2014). Persistent inflammation-induced upregulation of BDNF promotes synaptic delivery of α-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid receptor GluA1 subunits in descending pain modulatory circuits. J Biol Chem.

Zhang L et. al. (March 2013). Epigenetic activation of the MiR-200 family contributes to H19-mediated metastasis suppression in hepatocellular carcinoma. Carcinogenesis. 34(3):577-86.

Moreno R et. al. (March 2011). The β-interferon scaffold attachment region confers high-level transgene expression and avoids extinction by epigenetic modifications of integrated provirus in adipose tissue-derived human mesenchymal stem cells. Tissue Eng Part C Methods. 17(3):275-87.

Li W et. al. (November 2010). IL-32: a host proinflammatory factor against influenza viral replication is upregulated by aberrant epigenetic modifications during influenza A virus infection. J Immunol. 185(9):5056-65.

Molteni R et. al. (March 2010). Reduced function of the serotonin transporter is associated with decreased expression of BDNF in rodents as well as in humans. Neurobiol Dis. 37(3):747-55.

Deng L et. al. P-glycoprotein Mediates Postoperative Peritoneal Adhesion Formation by Enhancing Phosphorylation of the Chloride Channel-3 Theranostics. 6(2):204-218.

Customer Reviews

Rating By r*****@uga.edu Verified Purchase Reviewed on: Wednesday 24 June, 2015
Application Description
Chromatin Immunoprecipitation for Histone H3 acetylation analysis.

Other Thoughts
The sensitivity of the product is pretty decent, but the quantities of the components really bothers me. Same issue with other similar kits like P-2012.

The P-2010 kit I have is for 48 reactions, that is for 48 samples.

The protocol mentions to include "input" DNA samples along with the ChIP samples for most part of the protocol, except for plate-well immunoprecipitation. However, the reagents included in the kit are not sufficient if included the "input" too.

For example, if I have 40 samples and I include the "inputs" too, it makes it 80 samples. So this would need me to buy a whole another kit or buy individual components except the antibody, just for the "inputs".

Thank you,
R*****
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