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EpiQuik Global Histone H3 Phosphorylation (Ser10) Assay Kit (Fluorometric)

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Schematic procedure for using the EpiQuik Global Histone H3 Phosphorylation (Ser10) Assay Kit (Fluorometric).
Input Type: Cell Extracts, Histone Extracts, Unisolated Samples
Research Area: Phosphorylation
Target Application: Amount Quantitation
Vessel Format: 96-Well Plate
100% Guarantee: 6 months
Catalog No.SizePriceQty
P-7003-4848 assays $266.00 
P-7003-9696 assays $493.00 
Availability: Usually Ships In 1-2 Days 
Product Overview

The EpiQuik™ Global Histone H3 Phosphorylation (Ser10) Assay Kit (Fluorometric) is a convenient set of tools that allows the experimenter to measure global phospho histone H3 (ser10). The kit is ready-to-use and provides all the essential components needed for specifically measuring global histone H3 phophorylation at ser10 using a variety of mammalian cells (human, mouse, etc.) including fresh and frozen tissues, and cultured adherent and suspension cells. The kit has the following advantages:

  • Quick and efficient procedure, which can be finished within 3 hours.
  • Innovative fluorometric assay without the need for radioactivity, electrophoresis, or chromatography.
  • Specifically captures phospho histone H3 (ser10) with the detection limit as low as 2 ng/well.
  • The control is conveniently included for the quantification of phosphorylated histone H3 (ser10).
  • Strip microplate format makes the assay flexible: manual or high throughput.
  • Simple, reliable, and consistent assay conditions.

Principle & Procedure
The EpiQuik™ Global Histone H3 Phosphorylation (Ser10) Assay Kit (Fluorometric) is designed for measuring global histone H3 phosphorylation at ser10. In an assay with this kit, the phosphorylated histone H3 at ser10 is captured to the strip wells coated with an anti-phospho histone H3 (ser10) antibody. The captured phospho histone H3 (ser10) can then be detected with a labeled detection antibody, followed by a color development reagent. The ratio of phospho histone H3 (ser10) is proportional to the intensity of absorbance. The absolute amount of phospho histone H3 (ser10) can be quantitated by comparing to the standard control.

 
Fig. 1. Schematic procedure for using the EpiQuik Global Histone H3 Phosphorylation (Ser10) Assay Kit (Fluorometric).

Product Components

F1 (10X Wash Buffer)
F2 (Antibody Buffer)
F3 (Detection Antibody, 1 mg/ml)*
F4 (Fluoro Developer)*
F5 (Fluoro Enhancer)*
F6 (Fluoro Dilution)
Standard Control (100 µg/ml)*
8-Well Sample Strips (with Frame)
8-Well Standard Control Strips
User Guide

* For maximum recovery of the products, centrifuge the original vial prior to opening the cap.

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Material Safety Data Sheet]
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