Histone H3 Polyclonal Antibody

Background
The primary building block of chromatin is the nucleosome which is made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4). Histones thereby play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated through an intricate series of post-translational modifications of histones, known as the histone code, and nucleosome remodeling. Histone H3 is mainly acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. 

Description
Histone H3 Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Immunogen
Recombinant Protein

Purification
Affinity Purified

Specificity
Human

Isotype
IgG

Uniprot ID
P68431, Q71DI3, P84243

Alternative Names
Anti-histone H3, H3 histone antibody

Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Application
WB, ELISA; Recommended dilution: WB: 1:500-1:2000, ELISA 1:40000