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Histone H3 Polyclonal Antibody

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Histone H3 is one of five main histone proteins involved in chromatin structure in eukaryotic cells

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Western blot analysis on A: HepG2, B: NIH/3T3, C: Rat liver, cell lysates using the Histone H3 pAb.
Application: WB
Areas of Research: Unmodified Histone
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Affinity Purified
Reactivity: Human, Mouse, Rat
100% Guarantee: 6 months
Catalog No.SizePriceQty
A-1112-100100 ug $369.00 
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Product Overview

Background
The primary building block of chromatin is the nucleosome which is made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4). Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated through an intricate series of post-translational modifications of histones, known as the histone code, and nucleosome remodeling. Histone H3 is mainly acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. 

Description
Rabbit polyclonal antibody to Histone H3.

Formulation
Liquid. In TBS, pH 7.4, containing 0.5% BSA, 40% Glycerol, 0.05% sodium azide.

Immunogen
Synthetic peptide (KLH-coupled) corresponding to a region of Histone H3.

Purification
Affinity Purified

Specificity

Human, Mouse, Rat

Isotype
IgG

Alternative Names
Anti-histone H3, H3 histone antibody

Storage
Store at 4°C. Aliquot and store at -20°C or -80°C. Avoid multiple freeze/thaw cycles.

Application
WB: 1:1000-1:2000

JHB0002


Fig. 1. Western blot analysis on A: HepG2, B: NIH/3T3, C: Rat liver, cell lysates using the Histone H3 pAb.

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