Histone H3.3 Monoclonal Antibody [RMC109B]

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IHC image of Histone H3.3 Monoclonal Antibody [RMC109B] diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of Histone H3.3 Monoclonal Antibody [RMC109B] diluted at 1:100 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of Hela cells with Histone H3.3 Monoclonal Antibody [RMC109B] at 1:60, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Application:	ELISA, IF, IHC
Areas of Research:	Unmodified Histone
Clonality:	Monoclonal
Conjugate:	Unconjugated
Host:	Rabbit
Isotype:	IgG
Purification:	Affinity Purified
Reactivity:	Human
100% Guarantee:	6 months

Catalog No.	Size	Price	Qty
A68425-050	50 µl	$249.00
A68425-100	100 µl	$399.00

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Product Overview
User Guide & MSDS

Product Overview

Background
Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Description
Histone H3.3 Monoclonal Antibody , clone RMC109B. Unconjugated. Raised in: Rabbit.

Concentration
Inquire

Formulation
Liquid. Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Specificity
Human

Isotype
IgG

Uniprot ID
P84243

Purification
Affinity Purified

Immunogen
A synthesized peptide

Storage
Shipped at 4°C. Upon receipt, store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.

Alternative Names
Histone H3.3, H3F3A, H3.3A, H3F3, PP781, AND, H3F3B, H3.3B

Application
ELISA, IHC, IF; Recommended dilution: IHC: 1:50-1:500, IF: 1:30-1:200

VCU0006

User Guide & MSDS

[User Guide]*
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Histone H3.3 Monoclonal Antibody [RMC109B]