Histone H3K79me3 (H3K79 Trimethyl) Polyclonal Antibody

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Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K79 antibody.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K79 antibody at 1:1000 dilution.

Immunohistochemistry of paraffin-embedded human mammary cancer using TriMethyl-Histone H3-K79 antibody at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded rat testis using TriMethyl-Histone H3-K79 antibody at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded mouse testis using TriMethyl-Histone H3-K79 antibody at dilution of 1:200 (40x lens).

Immunofluorescence analysis of 293T cells using TriMethyl-Histone H3-K79 antibody. Blue: DAPI for nuclear staining.

Chromatin immunoprecipitation analysis of extracts of 293 cell line, using H3K79me3 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Application:	ChIP, DB, IF, IHC, WB
Areas of Research:	Histone Methylation & Demethylation
Clonality:	Polyclonal
Conjugate:	Unconjugated
Host:	Rabbit
Isotype:	IgG
Purification:	Affinity Purified
Reactivity:	Broad Range, Human, Mouse, Rat
100% Guarantee:	6 months

Catalog No.	Size	Price	Qty
A-4045-025	25 µl	$178.00
A-4045-050	50 µl	$344.00
A-4045-100	100 µl	$477.00

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Product Overview
Datasheet & SDS
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Product Overview

Description
Histone H3K79me3 (H3K79 Trimethyl) Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.

Specificity
Broad Range, Mouse, Rat, Human

Isotype
IgG

Uniprot ID
Q16695

Purification
Affinity Purified

Immunogen
A synthetic trimethylated peptide around K79 of human histone H3 (NP_003520.1)

Storage
Shipped at 4°C. Upon receipt, store at -20°C. Avoid repeated freeze.

Alternative Names
HIST3H3, H3/g

Application
DB, WB, IHC, IF, ChIP; Recommended dilution: DB 1:500 - 1:2000, WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:50 - 1:200, ChIP 1:50 - 1:200

Datasheet & SDS

Datasheet/User Guide
If the above file is not available online, you can request a copy by contacting us.

[Safety Data Sheet]

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Histone H3K79me3 (H3K79 Trimethyl) Polyclonal Antibody