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EpiQuik Total Histone H3 Acetylation Detection Fast Kit (Colorimetric)

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Suggested Workflow
Histone Protein Extraction
 
 
Histone H3 Acetylation Quantification
 
Schematic procedure for using the EpiQuik Total Histone H3 Acetylation Detection Fast Kit (Colorimetric).
Total histone H3 acetylation assay conducted using the EpiQuik™ Total Histone H3 Acetylation Detection Fast Kit (Colorimetric) with different concentrations of standard control proteins. OD of each point is average of three different tests.
Input Type: Histone Extracts
Research Area: Histone Acetylation
Target Application: Amount Quantitation
Vessel Format: 96-Well Plate
100% Guarantee: 6 months
Catalog No.SizePriceQty
P-4030-4848 assays $270.00 
P-4030-9696 assays $490.00 
Availability: Usually Ships In 1-2 Days 
Product Overview

The EpiQuik™ Total Histone H3 Acetylation Detection Fast Kit (Colorimetric) is a convenient package of tools that allows the experimenter to colorimetrically detect if histone H3 is acetylated, and colorimetrically quantify the amount of the acetylated histone H3. The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment. The EpiQuik™ Total Histone H3 Acetylation Detection Fast Kit (Colorimetric) is suitable for specifically measuring total histone H3 acetylation using a variety of mammalian cells (human, mouse, etc.) including fresh and frozen tissues, and cultured adherent and suspension cells. The kit has the following advantages:

  • Quick and efficient procedure, which can be finished within 2.5 hours.
  • Innovative colorimetric assay without the need for radioactivity, electrophoresis, or chromatography.
  • Captures histone H3 acetylated at any lysine site with the detection limit as low 2 ng/well and detection range from 5 ng-2 µg/well of histone extracts.
  • The control is conveniently included for the quantification of the amount of acetylated histone H3.
  • Strip microplate format makes the assay flexible: manual or high throughput.
  • Simple, reliable, and consistent assay conditions.

Principle & Procedure
The EpiQuik™ Total Histone H3 Acetylation Detection Fast Kit (Colorimetric) is designed for measuring total histone H3 acetylation in a fast format. In an assay with this kit, the acetyl histone H3 is captured to the strip wells coated with an anti-acetyl histone H3 antibody. The captured acetyl histone H3 can then be detected with a labeled detection antibody followed by a color development reagent. The ratio of acetyl histone H3 is proportional to the intensity of absorbance. The absolute amount of acetyl histone H3 can be quantified by comparing to the standard control.

Product Components

 

C1 (10X Wash Buffer)
C2 (Antibody Buffer)
C3 (Detection Antibody, 1 mg/ml)*
C4 (Color Developer)
C5 (Stop Solution)
Standard Control (100 µg/ml)*
8-Well Sample Strips (with Frame)
8-Well Standard Control Strips
User Guide

 * For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap.

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Material Safety Data Sheet]
Customer Reviews

Rating via Distributor/Reseller Verified Purchase Reviewed on: Tuesday 21 November, 2017
Application Description
We analyzed the histone modifications associated with biologic drugs treatment in different time points of the treatment following the manufacturers´ instructions. It is ready to use, easy and fast to perform and the instructions are clear.

Other Thoughts
The amount of Antibody buffer (C2) is quite restrictive. The kit does not include enough volume to perform pre-dilutions of the samples. When we measured the background of the empty plates that had been at room temperature for at least 15 minutes, you can see that the background is not homogenous. As the expected differences between our case and control samples are quite small, the variability found in the background may mask the specific differences within them. Nevertheless, I guess that it is difficult to obtain a totally homogenous background.It is important to underlie that all materials needed are included in the kit. To carry out the experiments you only need a microplate spectrophotometer and a centrifuge.


Differences in the percentage of acetylated H3 in peripheral blood mononuclear cells between our case and control subjects.


Differences in the percentage of acetylated H3 in peripheral blood mononuclear cells of healthy subjects.
Introduction to Epigenetics
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