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Methylamp MS-qPCR Fast Kit

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Schematic procedure for using the Methylamp MS-qPCR Fast Kit.
MS-PCR amplification of a gene region in euchromatin with internal control primers. Successful GpC methylation and bisulfite conversion were carried out using an EpiQuik Chromatin Accessibility & Methylome Sequencing (CAMe-Seq) Kit (Cat. No. P-1048) and PCR was performed using Methylamp™ MS- qPCR Fast Kit (Cat. No. P-1028).
Input Type: DNA
Research Area: DNA Methylation
Target Application: PCR Amplification
Vessel Format: 96-Well Plate, Columns/Tubes
100% Guarantee: 6 months
Catalog No.SizePriceQty
P-1028-100100 reactions $163.00 
P-1028-200200 reactions $297.00 
Availability: Usually Ships In 1-2 Days 
Product Overview

The Methylamp™ MS-qPCR Fast Kit is a complete set of essential components, which enables the experimenter to perform fast, specific, sensitive and reproducible methylation-specific quantitative PCR. The Methylamp™ MS-qPCR Fast Kit is very suitable for quantitative methylation-specific PCR in a fast format using very minute amounts of DNA. The novel hot start DNA polymerase contained in this kit can specifically reduce the overall time required for MS-qPCR from approximately 2.5 hours to less than 70 minutes. The kit also facilitates sensitivity and specificity of MS-qPCR by significantly increasing primer-bisulfite DNA template annealing, while simultaneously reducing non-specific annealing. This results in considerable time savings and a more efficient MS- qPCR. The kit has the following advantages:

  • An extremely fast procedure that can be finished within 70 minutes.
  • Abundant yields due to high amplification efficiency.
  • Highly accurate and specific in MSP, with reduced false- positive results.
  • Convenient master mix format to allow easy reaction setup.
  • Simple, reliable, and consistent assay conditions.
  • Can be used with any block-based real-time PCR instrument.

Principle & Procedure
The Methylamp™ MS-qPCR Fast Kit provides a master mix format, which contains a hot start DNA polymerase, dNTPs, an MS-PCR enhancer, an optimized buffer, and an intercalating green dye. This master mix allows for a convenient and easy reaction setup. The unique hot start DNA polymerase is provided in an inactive state at ambient temperature and is reactivated by several minute incubations at 95°C, which can easily be integrated into existing thermal cycling steps. The hot start DNA polymerase in combination with the optimized buffer ensures the MS-qPCR specificity and sensitivity. The green dye allows for DNA detection and analysis without using a sequence-specific probe.

 
Fig. 1. Schematic procedure for using the Methylamp MS-qPCR Fast Kit.

Fig. 2. MS-PCR amplification of a gene region in euchromatin with internal control primers. Successful GpC methylation and bisulfite conversion were carried out using an EpiQuik Chromatin Accessibility & Methylome Sequencing (CAMe-Seq) Kit (Cat. No. P-1048) and PCR was performed using MethylampMS- qPCR Fast Kit (Cat. No. P-1028).

Product Components

Master Mix (2X)
DNA/RNA-Free Water
Control Primers (β-actin)
Forward (100 µM)
Reverse (100 µM)
User Guide

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

Product Citations

Minning C et. al. (August 2014). Exploring breast carcinogenesis through integrative genomics and epigenomics analyses. Int J Oncol.

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