The Methylamp™ 96 DNA Modification Kit is a complete set of essential components which enables the experimenter to modify DNA efficiently. The entire procedure can be completed within 2 hours and 30 minutes and produces far superior results than any competitor kits. The Methylamp™ 96 DNA Modification Kit is suitable for MS-PCR, real time MS-PCR, methylation sequencing, and pyrosequencing, as well as methylation microarray.The kit has the following advantages:
Principle & ProcedureThe Methylamp™ 96 DNA Modification Kit contains all reagents required for bisulfite conversion on a DNA sample. DNA is denatured by heating, which allows DNA denaturation and bisulfite modification to be carried out simultaneously. In the modification process, bisulfite reagent reacts specifically with single-stranded DNA, thereby deaminating cytosine and creating a uracil residue. The unique DNA protection reagents contained in the modification buffer can prevent the chemical and thermophilic degradation of DNA in the bisulfite treatment. The non-toxic modified DNA capture buffer enables DNA to tightly bind to the column filter, thus DNA cleaning can be carried out on the column to effectively remove residual sodium bisulfite and salts. Modified DNA then can be eluted and stably stored at -20°C for up to 2 months.
Fig. 1. Schematic procedure for using the Methylamp 96 DNA Modification Kit
Godler DE et. al. (April 2010). Methylation of novel markers of fragile X alleles is inversely correlated with FMRP expression and FMR1 activation ratio. Hum Mol Genet. 19(8):1618-32.