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EpiQuik HDAC8 Assay Kit (Colorimetric)

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Suggested Workflow
Nuclear Protein Extraction
 
 
HDAC Protein Quantification
 
Schematic procedure for using the EpiQuik HDAC8 Assay Kit (Colorimetric).
HDAC8 proteins were bound to microplate wells at different concentrations and measured using the EpiQuik™ HDAC8 Assay Kit.
Nuclear proteins were prepared from different human tissue samples. 5 µg of nuclear proteins were bound to microplate wells for HDAC8 level measurement using the EpiQuik™ HDAC8 Assay Kit.
Input Type: Nuclear Extracts, Purified Enzyme
Research Area: Histone Acetylation
Target Application: Amount Quantitation
Vessel Format: 96-Well Plate
100% Guarantee: 6 months
Catalog No.SizePriceQty
P-4007-4848 assays $369.00 
P-4007-9696 assays $524.00 
Availability: Usually Ships In 1-2 Days 
Product Overview

The EpiQuik™ HDAC8 Assay Kit is a convenient package of tools that allows the experimenter to measure the amount of HDAC8 at tremendously fast speeds and consistency; superior and safer than all other current methods. The kit is ready-to-use and provides all the essential components needed to carry out a successful HDAC8 assay experiment without the need for electrophoresis and transfer process. It is suitable for measuring HDAC8 level from various fresh tissues and cultured mammalian cells. The kit has the following advantages:

  • Fast procedure, which can be finished within 3 hours.
  • Innovative colorimetric assay to semi-quantitatively measure HDAC8 amount without the need for electrophoresis.
  • Strip microplate format makes the assay flexible: manual or high throughput analysis.
  • Simple, reliable, and consistent assay conditions.

Principle & Procedure
The EpiQuik™ HDAC8 Assay Kit is designed for measuring total HDAC8 amount from tissues or cells. In an assay with this kit, the nuclear proteins containing HDAC8 are stably coated on the strip wells. The HDAC8 is recognized with a high-affinity specific antibody. The amount of HDAC8 can be quantified through a HRP conjugated secondary antibody-color development system and is proportional to the intensity of color development.

 
Fig. 1. Schematic procedure for using the EpiQuik HDAC8 Assay Kit (Colorimetric).

Fig. 2. HDAC8 proteins were bound to microplate wells at different concentrations and measured using the EpiQuik™ HDAC8 Assay Kit.

Fig. 3. Nuclear proteins were prepared from different human tissue samples. 5 µg of nuclear proteins were bound to microplate wells for HDAC8 level measurement using the EpiQuik™ HDAC8 Assay Kit.

Product Components

HC1 (10X Wash Buffer)
HC2 (HDAC Assay Buffer)
HC3 (Blocking Buffer)
HC4 (Capture Antibody, 200 µg/ml)*
HC5 (Detection Antibody, 200 µg/ml)*
HC6 (Developing Solution)
HC7 (Stop Solution)
HDAC8 Control (100 ng/µl)
8-Well Assay Strip (with Frame)
User Guide

* For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap.

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Material Safety Data Sheet]
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