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MuERVL-Gag Polyclonal Antibody

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Epifluorescence IF image using MuERVL-Gag Polyclonal Antibody in mouse preimplantation embryos from the 1 cell - 4 cell stages.
ICC staining MuERVL-Gag in mouse 3-cell stage embryo. The cells were incubated overnight at 4°C with MuERVL-Gag Polyclonal Antibody at a 1/100 dilution (shown in red) and phalloidin (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Application: ICC, IF
Areas of Research: Other Antibodies
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Affinity Purified
Reactivity: Mouse
100% Guarantee: 6 months
Catalog No.SizePriceQty
A-2801-100100 µl $359.00 
Availability: Usually Ships In 1-2 Days 
Product Overview

Background
When the zygotic genome is first transcribed, a large number of retrotransposons are expressed, including the endogenous retroviruses (ERVs), LINE-1 elements, and the non-autonomous SINE elements. MuERV-L/MERVL retrovirus-like particles are transiently de-repressed and produce 3% of the transcribed mRNAs at the two-cell (2C) stage. Right after the 2C stage, the expression of MERVL-retroelement is silenced. It has been found that this regulated pattern of MERVL expression overlapped with more than one hundred 2C-specific genes that have co-opted regulatory elements from these foreign retroviruses to begin their transcription.  

Description
Rabbit polyclonal antibody to MuERVL-Gag.

Formulation
1*TBS (pH7.4), 0.5% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. 

Specificity
Mouse

Isotype
IgG

Purification
Affinity purified

Storage
Store at 4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Alternative Names
Murine Endogenous Retrovirus-Gag

Application
IF (1:100 - 1:500), ICC (1:100-1:200)

JHB0002

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing [email protected] along with your contact information and institution name.

Customer Reviews

Rating by k*****@berkeley.edu Verified Purchase Reviewed on: Thursday 06 July, 2017
Application Description
The antibody was used for IF to look at the subcellular localization of the protein. Very clean images were obtained, no complaints.

Procedural Details
Female mice, aged 4 weeks were superovulated with PSMG and HCG injections spaced 48hrs apart. preimplantation embryos were harvested 20hrs post HCG injection and in-vitro cultured in KSOM until desired developmental stage was reached. Embryos were fixed with 4% PFA supplemented with 0.3% BSA. Embryos were then treated with primary antibody at a 1:400 dilution in 0.3% BSA in PBS and incubated overnight. Embryos were then incubated with Alexa Fluor 647 or 488 secondary antibodies for 1-2hrs and then washed thoroughly in 0.3% BSA + PBS. Embryos were then treated with DAPI for 15 minutes, washed again and mounted onto slides ready for visualization.

Other Thoughts
Would like to know the specific peptide sequence used to generate the antibody if that information is available.


Epifluorecence IF image using MuERV-L polyclonoal antibody (epigentek) in mouse preimlantation embryos from the 1 cell - 4 cell stages
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