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B-tubulin Monoclonal Antibody [A1-A4]

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Confocal IF image using B-tubulin Monoclonal Antibody [A1-A4] in an early 2 cell mouse embryo.
Western blot analysis on
1: NIH/3T3
2: Hela
lysates using B-tubulin Monoclonal Antibody [A1-A4].
Immunofluorescent staining on SKOV-3 cells using B-tubulin Monoclonal Antibody [A1-A4].
Application: ICC, IF, WB
Areas of Research: Cellular Structure
Clonality: Monoclonal
Conjugate: Unconjugated
Host: Mouse
Isotype: IgG3
Purification: Affinity Purified
Reactivity: Human, Mouse, Rat
100% Guarantee: 6 months
Catalog No.SizePriceQty
A-0801-100100 µl $399.00 
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Product Overview

Background 
Tubulin is one of several members of a small family of globular proteins. The most common members of the tubulin family are α-tubulin and β-tubulin. The beta-tubulin (relative molecular weight about 50 kDa) is counterpart of alpha-tubulin in tubulin heterodimer, it is coded by multiple tubulin genes and it is also posttranslationally modified. Heterogeneity of subunit is concentrated in C-terminal structural domain. Beta-Tubulin may have bound GTP or GDP. Under certain conditions B-tubulin can hydrolyze its bound GTP to GDP plus Pi, release the Pi, and exchange the GDP for GTP. 

Description
Mouse monoclonal antibody to B-tubulin, clone A1-A4

Specificity
Human, Mouse, Rat

Isotype
IgG3

Uniprot ID
Q9D6F9, P07437

Immunogen
Synthetic peptide (KLH-coupled)

Formulation
Liguid. 1*TBS (pH7.4), 0.5% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage
Store at –20°C

Alternative Names
β tubulin, Tubulin beta-5 chain, beta 5-tubulin, beta Ib tubulin, beta-4 tubulin, tubulin beta polypeptide, tubulin beta-1 chain, tubulin, beta, tubulin, beta polypeptide, Anti B-tubulin 

Application
WB: 1:5,000-1:10,000 
ICC: 1:200-1:500
IF: 1:100-1:500

JHB0002

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

Customer Reviews

Rating by k*****@berkeley.edu Verified Purchase Reviewed on: Thursday 06 July, 2017
Application Description
The antibody was used for IF to look at the subcellular localization of the protein. Very clean images were obtained, no complaints.

Procedural Details
Female mice, aged 4 weeks were superovulated with PSMG and HCG injections spaced 48hrs apart. preimplantation embryos were harvested 20hrs post HCG injection and in-vitro cultured in KSOM until desired developmental stage was reached. Embryos were fixed with 4% PFA supplemented with 0.3% BSA. Embryos were then treated with primary antibody at a 1:400 dilution in 0.3% BSA in PBS and incubated overnight. Embryos were then incubated with Alexa Fluor 647 or 488 secondary antibodies for 1-2hrs and then washed thoroughly in 0.3% BSA + PBS. Embryos were then treated with DAPI for 15 minutes, washed again and mounted onto slides ready for visualization.


Confocal IF image using B-tubulin monoclonal antibody (epigentek) in an early 2 cell mouse embryo.
DNMT ELISA Easy Kit

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