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N6-methyladenosine (m6A) Polyclonal Antibody

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ELISA performed at 1:1000 dilution using N6-methyladenosine (m6A) Polyclonal Antibody.
Application: ELISA, ICC, IF, IP
Areas of Research: RNA Methylation
Clonality: Polyclonal
Conjugate: HRP, Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Affinity Purified
Reactivity: Broad Range, Human, Mouse
100% Guarantee: 6 months
Catalog No.SizePriceQty
A-1801-02020 µl $99.00 
A-1801-100100 µl $339.00 
Availability: Usually Ships In 1-2 Days 
Product Overview

Background
N6-methyladenosine (m6A) is the most common and abundant modification in RNA molecules present in eukaryotes. The m6A modification is catalyzed by a methyltransferase complex METTL3 and removed by the recently discovered m6A RNA demethylases FTO and ALKBH5, which catalyze m6A demethylation in an α-ketoglutarate (α-KG)- and Fe2+-dependent manner. It was shown that METTL3, FTO, and ALKBH5 play essential roles in many biological processes, ranging from development and metabolism to fertility. m6A accounts for more than 80% of all RNA base methylations and exists in various species. m6A is mainly distributed in mRNA and also occurs in non-coding RNA such as tRNA, rRNA, and snRNA. The relative abundance of m6A in mRNA transcripts has been shown to affect RNA metabolism processes such as splicing, nuclear export, translation ability and stability, and RNA transcription. Abnormal m6A methylation levels induced by defects in m6A RNA methylase and demethylase could lead to dysfunction of RNA and diseases. For example, abnormally low levels of m6A in target mRNAs due to increased FTO activity in patients with FTO mutations, through an as-yet-undefined pathway, contributes to the onset of obesity and related diseases. The dynamic and reversible chemical m6A modification in RNA may also serve as a novel epigenetic marker of profound biological significance. Therefore, more useful information for a better understanding of m6A RNA methylation levels and distribution on RNA transcripts could benefit diagnostics and therapeutics of disease.

Description
N6-methyladenosine (m6A) Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Liquid. PBS, PH 7.4.

Specificity
Mouse, Human, Broad Range

Isotype
IgG

Purification
Affinity purified

Immunogen
KLH-conjugated N6-methyladenosine (m6A)

Storage
Shipped at 4°C. Upon delivery aliquot and store at 4°C (short-term), or store at  20°C or -80°C (long-term). Avoid repeated freeze.

Alternative Names
Anti-N6-methyladenosine, N6-methyladenosine, N6(methylnitroso)adenosine, m6A, N6-methyladenosine, 6mA

Application
ELISA, IF, ICC, IP; Recommended dilution: ELISA 1:1000, IF: 1:50 - 1:100, ICC: 1:50 - 1:100, IP: 1:50 - 1:100

 

User Guide & MSDS

[User Guide]*
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