Epigentek Home
 
EpiGentek Guarantee

EpiQuik Dnmt3A Assay Kit

Share:

For measuring the amount of DNMT3A protein in samples

Citations (9) | Write a Review
Suggested Workflow
Nuclear Protein Extraction
 
 
DNMT Assay
 
Schematic procedure for using the EpiQuik Dnmt3A Assay Kit.
Nuclear extracts were prepared from MCF-7 cells using the EpiQuik Nuclear Extraction Kit and total amount of Dnmt was measured.
Input Type: Nuclear Extracts, Purified Enzyme
Research Area: DNA Methylation
Target Application: Amount Quantitation
Vessel Format: 96-Well Plate
100% Guarantee: 6 months
Catalog No.SizePriceQty
P-3012-248 assays $409.00 
P-3012-396 assays $579.00 
Order now & get it by Thursday, October 17th  
Product Overview

The EpiQuik™ Dnmt3A Assay Kit is a convenient package of tools that allows the experimenter to measure the amount of Dnmt3A at tremendously fast speeds and consistency; superior and safer than all other current methods. The kit is ready-to-use and provides all the essential components needed to carry out a successful Dnmt3A assay experiment. The EpiQuik™ Dnmt3A Assay Kit is suitable for measuring Dnmt amounts quantitatively from fresh tissue and cultured cells of human and mouse. The kit has the following advantages:

  • The fastest procedure, which can be finished within 3 hours.
  • Innovative colorimetric assay, which quantitatively measure Dnmt3A amount without the need for electrophoresis.
  • Strip microplate format makes the assay flexible: manual or high throughput analysis.
  • Simple, reliable, and consistent assay conditions.

Principle & Procedure
The EpiQuik™ Dnmt3A Assay Kit is designed for measuring total Dnmt3A amount from tissues or cells. In an assay with this kit, the unique Dnmt affinity substrate is stably coated on the strip well. The sample is added into the well and Dnmt3A contained in the sample binds to the substrate. The bound Dnmt3A can be recognized with a specific Dnmt3A antibody and colorimetrically quantified through an ELISA-like reaction. The amount of Dnmt3A is proportional to the intensity of color development.

 
Fig. 1. Schematic procedure for using the EpiQuik Dnmt3A Assay Kit.

Fig. 2. Nuclear extracts were prepared from MCF-7 cells using the EpiQuik Nuclear Extraction Kit and total amount of Dnmt was measured.

Product Components

DA1 (10X Wash Buffer)
DA2 (Assay Buffer)
DA3 (Dnmt3A Standard, 20 µg/ml)*
DA4 (Capture Antibody, 100 µg/ml)*
DA5 (Detection Antibody, 200 µg/ml)*
DA6 (Developing Solution)
DA7 (Stop Solution)
BB (Blocking Buffer)
8-Well Assay Strips (with Frame)
User Guide

* For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap.

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Safety Data Sheet]
Product Citations

Fukui T et. al. (March 2019). Extracellular Spermine Activates DNA Methyltransferase 3A and 3B. Int J Mol Sci. 20(5)

Mccoy CR et. al. (January 2019). Altered DNA Methylation in the Developing Brains of Rats Genetically Prone to High Versus Low Anxiety. J Neurosci.

Swathy B et. al. (January 2018). Understanding the influence of antipsychotic drugs on global methylation events and its relevance in treatment response. Epigenomics.

Coco M et. al. (November 2017). Effects of age and sex on epigenetic modification induced by an acute physical exercise. Medicine (Baltimore). 96(44):e8325.

Parira T et. al. (September 2017). Novel detection of post-translational modifications in human monocyte-derived dendritic cells after chronic alcohol exposure: Role of inflammation regulator H4K12ac. Sci Rep. 7(1):11236.

Mytych J et. al. (February 2016). Prolonged Effects of Silver Nanoparticles on p53/p21 Pathway-Mediated Proliferation, DNA Damage Response, and Methylation Parameters in HT22 Hippocampal Neuronal Cells. Mol Neurobiol.

Horsburgh S et. al. (December 2015). Exercise-conditioned plasma attenuates nuclear concentrations of DNA methyltransferase 3B in human peripheral blood mononuclear cells. Physiol Rep. 3(12)

Majid S et. al. (April 2009). BTG3 tumor suppressor gene promoter demethylation, histone modification and cell cycle arrest by genistein in renal cancer. Carcinogenesis. 30(4):662-70.

Roll JD et. al. (January 2008). DNMT3b overexpression contributes to a hypermethylator phenotype in human breast cancer cell lines. Mol Cancer. 7:15.

Introduction to Epigenetics
Related Products

Researchers Also Purchased
EpiQuik Dnmt1 Assay Kit
EpiQuik Dnmt1 Assay Kit
EpiQuik Dnmt3B Assay Kit
EpiQuik Dnmt3B Assay Kit
EpiQuik Nuclear Extraction Kit
EpiQuik Nuclear Extraction Kit
EpiQuik DNA Methyltransferase (DNMT) Activity/Inhibition Assay Kit
EpiQuik DNA Methyltransferase (DNMT) Activity/Inhibition Assay Kit
Terms & Conditions | Privacy Policy | Site Map
Copyright © 2019 EpiGentek Group Inc. All rights reserved.