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EpiQuik Dnmt3B Assay Kit

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For measuring the amount of DNMT3B protein in samples

Citations (9) | Write a Review
Suggested Workflow
Nuclear Protein Extraction
 
 
DNMT Assay
 
Schematic procedure for using the EpiQuik Dnmt3B Assay Kit.
Nuclear extracts were prepared from MCF-7 cells using the EpiQuik Nuclear Extraction Kit and total amount of Dnmt was measured.
Input Type: Nuclear Extracts, Purified Enzyme
Research Area: DNA Methylation
Target Application: Amount Quantitation
Vessel Format: 96-Well Plate
100% Guarantee: 6 months
Catalog No.SizePriceQty
P-3013-248 assays $409.00 
P-3013-396 assays $579.00 
Order now & get it by Thursday, August 29th  
Product Overview

The EpiQuik™ Dnmt3B Assay Kit is a convenient package of tools that allows the experimenter to measure the amount of Dnmt3B at tremendously fast speeds and consistency; superior and safer than all other current methods. The kit is ready-to-use and provides all the essential components needed to carry out a successful Dnmt3B assay experiment. The EpiQuik™ Dnmt3B Assay Kit is suitable for measuring Dnmt amounts quantitatively from fresh tissue and cultured cells of human and mouse. The kit has the following advantages:

  • The fastest procedure, which can be finished within 3 hours.
  • Innovative colorimetric assay, which quantitatively measure Dnmt3B amount without the need for electrophoresis.
  • Strip microplate format makes the assay flexible: manual or high throughput analysis.
  • Simple, reliable, and consistent assay conditions.

Principle & Procedure
The EpiQuik™ Dnmt3B Assay Kit is designed for measuring total Dnmt3B amount from tissues or cells. In an assay with this kit, the unique Dnmt affinity substrate is stably coated on the strip well. The sample is added into the well and Dnmt3B contained in the sample binds to the substrate. The bound Dnmt3B can be recognized with a specific Dnmt3B antibody and colorimetrically quantified through an ELISA-like reaction. The amount of Dnmt3B is proportional to the intensity of color development.

 
Fig. 1. Schematic procedure for using the EpiQuik Dnmt3B Assay Kit.

Fig. 2. Nuclear extracts were prepared from MCF-7 cells using the EpiQuik Nuclear Extraction Kit and total amount of Dnmt was measured.

Product Details

The EpiQuik™ Dnmt3B Assay Kit is a convenient package of tools that allows the experimenter to measure the amount of Dnmt3B at tremendously fast speeds and consistency; superior and safer than all other current methods. The kit is ready-to-use and provides all the essential components needed to carry out a successful Dnmt3B assay experiment. The EpiQuik™ Dnmt3B Assay Kit is suitable for measuring Dnmt amounts quantitatively from fresh tissue and cultured cells of human and mouse. The kit has the following advantages:

  • The fastest procedure, which can be finished within 3 hours.
  • Innovative colorimetric assay, which quantitatively measure Dnmt3B amount without the need for electrophoresis.
  • Strip microplate format makes the assay flexible: manual or high throughput analysis.
  • Simple, reliable, and consistent assay conditions.

Principle & Procedure
The EpiQuik™ Dnmt3B Assay Kit is designed for measuring total Dnmt3B amount from tissues or cells. In an assay with this kit, the unique Dnmt affinity substrate is stably coated on the strip well. The sample is added into the well and Dnmt3B contained in the sample binds to the substrate. The bound Dnmt3B can be recognized with a specific Dnmt3B antibody and colorimetrically quantified through an ELISA-like reaction. The amount of Dnmt3B is proportional to the intensity of color development.

 
Fig. 1. Schematic procedure for using the EpiQuik Dnmt3B Assay Kit.

Fig. 2. Nuclear extracts were prepared from MCF-7 cells using the EpiQuik Nuclear Extraction Kit and total amount of Dnmt was measured.

Product Components

DB1 (10X Wash Buffer)
DB2 (Assay Buffer)
DB3 (Dnmt3B Standard, 20 µg/ml)*
DB4 (Capture Antibody, 100 µg/ml)*
DB5 (Detection Antibody, 200 µg/ml)*
DB6 (Developing Solution)
DB7 (Stop Solution)
BB (Blocking Buffer)
8-Well Assay Strips (with Frame)
User Guide

* For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap.

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Safety Data Sheet]
Product Citations

Mccoy CR et. al. (January 2019). Altered DNA Methylation in the Developing Brains of Rats Genetically Prone to High Versus Low Anxiety. J Neurosci.

Coco M et. al. (November 2017). Effects of age and sex on epigenetic modification induced by an acute physical exercise. Medicine (Baltimore). 96(44):e8325.

Cechinel LR et. al. (October 2016). Treadmill exercise induces age and protocol-dependent epigenetic changes in prefrontal cortex of Wistar rats. Behav Brain Res. 313:82-7.

Mytych J et. al. (February 2016). Prolonged Effects of Silver Nanoparticles on p53/p21 Pathway-Mediated Proliferation, DNA Damage Response, and Methylation Parameters in HT22 Hippocampal Neuronal Cells. Mol Neurobiol.

Horsburgh S et. al. (December 2015). Exercise-conditioned plasma attenuates nuclear concentrations of DNA methyltransferase 3B in human peripheral blood mononuclear cells. Physiol Rep. 3(12)

Zhao J et. al. (January 2013). Genome-wide identification of Epstein-Barr virus-driven promoter methylation profiles of human genes in gastric cancer cells. Cancer. 119(2):304-12.

Jin Y et. al. (June 2011). Homocysteine levels impact directly on epigenetic reprogramming in astrocytes. Neurochem Int. 58(7):833-8.

Majid S et. al. (April 2009). BTG3 tumor suppressor gene promoter demethylation, histone modification and cell cycle arrest by genistein in renal cancer. Carcinogenesis. 30(4):662-70.

Roll JD et. al. (January 2008). DNMT3b overexpression contributes to a hypermethylator phenotype in human breast cancer cell lines. Mol Cancer. 7:15.

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