The EpiQuik™ HDAC2 Assay Kit is a convenient package of tools that allows the experimenter to measure the amount of HDAC2 at tremendously fast speeds and consistency; superior and safer than all other current methods. The kit is ready-to-use and provides all the essential components needed to carry out a successful HDAC2 assay experiment without the need for electrophoresis and transfer process. It is suitable for measuring HDAC2 level from various fresh tissues and cultured mammalian cells. The kit has the following advantages:
Principle & ProcedureThe EpiQuik™ HDAC2 Assay Kit is designed for measuring total HDAC2 amount from tissues or cells. In an assay with this kit, the nuclear proteins containing HDAC2 are stably coated on the strip wells. The HDAC2 is recognized with a high-affinity specific antibody. The amount of HDAC2 can be quantified through a HRP conjugated secondary antibody-color development system and is proportional to the intensity of color development.
Fig. 1. Schematic procedure for using the EpiQuik HDAC2 Assay Kit (Colorimetric).
Fig. 2. HDAC2 proteins were bound to microplate wells at different concentrations and measured using the EpiQuik™ HDAC2 Assay Kit.
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