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CUT&RUN m6A MeRIP Assays

Enrich for m6A-containing RNA from low input with minimal non-specific background levels

RNA modifications can occur at various locations along an RNA sequence. Identifying where they are and what impact they may have on post-transcriptional regulation, including gene expression and mRNA control, can help researchers understand anomalies in various biological functions. Therefore, techniques that can quickly and accurately detect these modifications, especially m6A in RNA, are important for epigenetic research. meRIP and MeRIP-seq are two familiar methods used to detect m6A RNA methylation in the entire transcriptome range, but they have limitations and can be pricey. EpiGentek has improved upon these techniques, developing a newer method called CUT&RUN RNA m6A enrichment (meRIP). Using a specialized nuclease that enriches for m6A containing RNA, the process allows for high-resolution mapping in only a few hours.

What is CUT&RUN m6A meRIP?
This innovative approach combines the advantages of MeRIP-seq and miCLIP with EpiGentek’s proprietary EpiQuik technology for higher enrichment, lower input, reduced background, and a faster, more streamlined procedure. CUT&RUN m6A MeRIP uses a state-of-the-art RNA cleavage enzyme mix to simultaneously fragment immunocaptured RNA and cleave/remove any RNA sequences in both ends of the target m6A-containing sequences, without affecting RNA regions occupied by the antibody. Short RNA fragments are consequently generated only bound with anti-m6A antibody. True target m6A-enriched regions can therefore be reliably identified, and high-resolution mapping achieved.

Kit Details
Click image to see more details	EpiQuik CUT&RUN m6A RNA Enrichment (MeRIP) Kit High resolution m6A RNA enrichment based on CUT&RUN for increased specificity and signals Fast: The entire procedure is 2 hours and 3 min with less than 30 min of hands-on time High Enrichment: Generates short RNA fragments bound only to m6A antibody Low Sample Requirement: Input RNA can be as low as 500 ng Minimal Background: Data analysis with <10 million reads Catalog #: P-9018
Click image to see more details	EpiNext CUT&RUN RNA m6A-Seq Kit All-in-one m6A RNA enrichment and NGS library preparation based on CUT&RUN Fast: The entire Procedure is less than 6 hours with less than 1 hour of hands-on time High Enrichment: Generates short RNA fragments bound only to m6A antibody Low Sample Requirement: Input RNA can be as low as 500 ng Minimal Background: Data analysis with <10 million reads Library Prep: Includes cDNA library preparation components Catalog #: P-9016