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   Home  »  Global m6A & 5mC RNA Quantification Kits 
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Global RNA Quantification Kits

Accurately measure global m6A or 5-mC in RNA

Extraction
RNA
Global RNA Modification ELISAs
m6A
5mC
Site-specific Assays
Bisulfite conversion
MeRIP
qPCR
NGS Analysis
Nuclear Extracts
Enzyme Activity/Inhibition ELISAs
m6A methylases
m6A demethylases
» Comprehensive Solutions for RNA Modification Analysis

Expand upon your understanding of RNA methylation with the help of EpiGentek’s Global RNA Methylation Kits. From directly quantifying the "fifth RNA base" N6-Methyladenosine (m6A) to measuring 5-mC RNA, our kits make it easy for you to determine how RNA methylation influences your research project.

About m6A RNA Methylation

Investigate N6-methyladenosine (m6A) -- the most common and abundant methylation modification in RNA molecules present in eukaryotes. Known as the "fifth RNA base", m6A RNA modifications are reversible, highly dynamic, and likely play important biological roles comparable to DNA methylation and histone modification.

Schematic procedure for the EpiQuik CUT&RUN m6A RNA Enrichment (MeRIP) Kit (Cat# P-9018).

Schematic procedure for the EpiQuik m6A RNA Methylation Quantification Kit (Cat# P-9005).

About 5-mC RNA Methylation

Study the occurrence of 5-methylcytosine (5-mC) in RNA -- a well-known methylation modification found in various RNA molecules including tRNAs, rRNAs, mRNAs, and non-coding RNAs (ncRNAs). Evidence has shown that RNA cytosine methylation affects various biological processes, such as RNA stability and mRNA translation. The loss of 5-mC in vault RNAs may cause aberrant processing into Argonaute-associated small RNA fragments that can function as microRNAs.

Featured Kits

m6A MCF-7 RNA Human Brain RNA Yeast tRNA chart
EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)

For absorbance-based quantitation of N6-methyladenosine in RNA in an ELISA-like format

  • Fast: The entire procedure can be finished within 3 hours and 45 minutes
  • High Sensitivity: Detection limit can be as low as 10 pg of m6A
  • Convenient: Allows m6A quantification from both mRNA and ncRNA
  • High Specificity: No cross-reactivity to unmethylated adenosine within the indicated concentration range of the sample RNA
  • Universal Controls: Suitable for quantifying m6A from any species
  • Flexible: Strip-well microplate format makes the assay flexible: manual or high throughput

Catalog #: P-9005

View Kit
m6A RNA Delta RFU chart
EpiQuik m6A RNA Methylation Quantification Kit (Fluorometric)

For fluorescence-based measurement of N6-methyladenosine (m6A or 6mA) in RNA

  • Fast: The entire procedure can be finished within 3 hours and 45 minutes
  • High Sensitivity: Detection limit can be as low as 5 pg of m6A
  • Convenient: Allows m6A quantification from both mRNA and ncRNA
  • High Specificity: No cross-reactivity to unmethylated adenosine within the indicated concentration range of the sample RNA
  • Universal Controls: Suitable for quantifying m6A from any species
  • Flexible: Strip-well microplate format makes the assay flexible: manual or high throughput

Catalog #: P-9008

View Kit
m6A RNA Urine (ul) Chart
MethylFlash Urine m6A Quantification Kit

For absorbance-based quantitation of N6-methyladenosine in urine in an ELISA-like format

  • Fast: The entire procedure can be finished within 4 hours
  • High Sensitivity: Detection limit can be as low as 0.01 ng/assay well
  • Low Input: Urine volume for each assay can range from 1 to 20 µl, with 5 µl as optimal
  • High Specificity: No cross-reactivity to unmethylated adenosine
  • Universal Controls: Suitable for quantifying m6A from any species
  • Flexible: Strip-well microplate format makes the assay flexible: manual or high throughput

Catalog #: P-9015

View Kit
5-mC MS-LC Human Brain Mouse Brain Chart
EpiQuik 5mC RNA Methylation Quantification Kit (Colorimetric)

For absorbance-based quantitation of N6-methyladenosine in RNA in an ELISA-like format

  • Fast: The entire procedure only needs 2 hours and 40 minutes
  • Sensitive: Detection limit can be as low as 0.02% of 5-mC RNA from 200 ng of input RNA
  • Accurate: Highly comparable with HPLC-MS analysis
  • Specific: No cross-reactivity to unmethylated cytosine or hydroxymethylated cytosine within the indicated concentration range of the sample RNA
  • Universal Controls: Suitable for quantifying 5mC from any species
  • Flexible: Strip-well microplate format makes the assay flexible: manual or high throughput

Catalog #: P-9009

View Kit

Integrated Workflow Tools for RNA Methylation Research

Global RNA Modification ELISAs
Site-specific Assays
MeRIP
Enzyme Activity/Inhibition ELISAs


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