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Expand upon your understanding of RNA methylation with the help of EpiGentek’s Global RNA Methylation Kits. From directly quantifying the "fifth RNA base" N6-Methyladenosine (m6A) to measuring 5-mC RNA, our kits make it easy for you to determine how RNA methylation influences your research project.
About m6A RNA Methylation
Investigate N6-methyladenosine (m6A) -- the most common and abundant methylation modification in RNA molecules present in eukaryotes. Known as the "fifth RNA base", m6A RNA modifications are reversible, highly dynamic, and likely play important biological roles comparable to DNA methylation and histone modification.
Schematic procedure for the EpiQuik m6A RNA Methylation Quantification Kit (Cat# P-9005).
About 5-mC RNA Methylation
Study the occurrence of 5-methylcytosine (5-mC) in RNA -- a well-known methylation modification found in various RNA molecules including tRNAs, rRNAs, mRNAs, and non-coding RNAs (ncRNAs). Evidence has shown that RNA cytosine methylation affects various biological processes, such as RNA stability and mRNA translation. The loss of 5-mC in vault RNAs may cause aberrant processing into Argonaute-associated small RNA fragments that can function as microRNAs.
Featured Kits
EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)
For absorbance-based quantitation of N6-methyladenosine in RNA in an ELISA-like format
- Fast: The entire procedure can be finished within 3 hours and 45 minutes
- High Sensitivity: Detection limit can be as low as 10 pg of m6A
- Convenient: Allows m6A quantification from both mRNA and ncRNA
- High Specificity: No cross-reactivity to unmethylated adenosine within the indicated concentration range of the sample RNA
- Universal Controls: Suitable for quantifying m6A from any species
- Flexible: Strip-well microplate format makes the assay flexible: manual or high throughput
Catalog #: P-9005
EpiQuik m6A RNA Methylation Quantification Kit (Fluorometric)
For fluorescence-based measurement of N6-methyladenosine (m6A or 6mA) in RNA
- Fast: The entire procedure can be finished within 3 hours and 45 minutes
- High Sensitivity: Detection limit can be as low as 5 pg of m6A
- Convenient: Allows m6A quantification from both mRNA and ncRNA
- High Specificity: No cross-reactivity to unmethylated adenosine within the indicated concentration range of the sample RNA
- Universal Controls: Suitable for quantifying m6A from any species
- Flexible: Strip-well microplate format makes the assay flexible: manual or high throughput
Catalog #: P-9008
MethylFlash Urine m6A Quantification Kit
For absorbance-based quantitation of N6-methyladenosine in urine in an ELISA-like format
- Fast: The entire procedure can be finished within 4 hours
- High Sensitivity: Detection limit can be as low as 0.01 ng/assay well
- Low Input: Urine volume for each assay can range from 1 to 20 µl, with 5 µl as optimal
- High Specificity: No cross-reactivity to unmethylated adenosine
- Universal Controls: Suitable for quantifying m6A from any species
- Flexible: Strip-well microplate format makes the assay flexible: manual or high throughput
Catalog #: P-9015
EpiQuik 5mC RNA Methylation Quantification Kit (Colorimetric)
For absorbance-based quantitation of N6-methyladenosine in RNA in an ELISA-like format
- Fast: The entire procedure only needs 2 hours and 40 minutes
- Sensitive: Detection limit can be as low as 0.02% of 5-mC RNA from 200 ng of input RNA
- Accurate: Highly comparable with HPLC-MS analysis
- Specific: No cross-reactivity to unmethylated cytosine or hydroxymethylated cytosine within the indicated concentration range of the sample RNA
- Universal Controls: Suitable for quantifying 5mC from any species
- Flexible: Strip-well microplate format makes the assay flexible: manual or high throughput
Catalog #: P-9009
