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   Home  »  DNMT ELISA Kit for Measuring DNA Methyltransferase Activity and Inhibition 
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Banner showing enzymatic epigenetics workflow
EpiQuik DNMT Activity/Inhibition ELISA Easy Kit box and plate

EpiQuik™ DNMT Activity/Inhibition ELISA Easy Kit

A fast, sensitive, and convenient way to measure DNMT activity and inhibition with reliable, reproducible results.

Robust Detection of DNMT Activity/Inhibition

High sensitivity and specificity demonstrated with HeLa nuclear extracts
High sensitivity and specificity of DNMT activity/inhibition demonstrated with HeLa nuclear extracts using the EpiQuik™ DNMT Activity/Inhibition ELISA Easy Kit.
Assay sensitivity and specificity shown with M.SssI enzyme
DNMT activity/inhibition assay shows high sensitivity and specificity with M.SssI using the EpiQuik™ ELISA Easy Kit.

This ELISA-style kit quickly measures DNMT activity and inhibition directly from nuclear extracts or purified enzymes in about 2 hours.

  • Fast & simple: Complete the entire procedure in just ~2 h 10 min.
  • High sensitivity & specificity: Detects as low as 0.2 ng of purified enzyme.
  • Convenient: Ready-to-use format—no radioactivity or special tools.
  • Robust: Optimized kit yields a broader signal window and consistent results.
  • Flexible: 96 strip-well microplate format for manual or high-throughput analysis.

Consistent, reliable DNMT activity analysis—whether you’re working with a handful of samples or high-throughput workflows.

Compare DNMT Activity / Inhibition Assay Kits

Feature EpigenTek DNMT Activity/Inhibition Assay Kits Competitor A
Assay Principle ✅ Direct (immunoaffinity-based end-product measurement) ❌ Indirect (MBD protein binding to methylated DNA)
Sensitivity ✅ High (0.2 ng of enzyme) ❌ Low (0.5 ng of enzyme)
Signal/Noise Ratio ✅ High (>20 with very low background) ❌ Lower (<20)
Assay Accuracy with Cell Lysates ✅ Excellent (no antibody cross-reactivity) ❌ Poor (MBD2 cross-reacts with unmethylated DNA)
Accuracy of Enzyme Inhibition Assay ✅ High (direct detection → amount change of end-product) ❌ Low (indirect detection → MBD protein)
Peer-reviewed Citations ✅ Very high (350+*) ❌ Low
Also in fluorometric format ✅ Yes (cat #P-3004/3010) ❌ No

*Including all previous versions.

Superior DNMT Activity Detection—2× Faster, 2.5× More Sensitive

EpiQuik DNMT assay speed comparison
Fast protocol enables rapid DNMT activity calls.
EpiQuik DNMT assay sensitivity comparison
Excellent sensitivity across a wide dynamic range.

Featured in the world’s leading scientific journals

Our DNMT Activity/Inhibition ELISA Kit is trusted by leading researchers and cited in top-tier publications with high impact factors.

Nature Immunology

Impact Factor: 31.25

Zhao Y et al. (Sep 2021). Transposon-triggered innate immune response confers cancer resistance to the blind mole rat. Nat Immunol.

Acta Neuropathologica

Impact Factor: 18.2

Volpatti JR et al. (Sep 2022). X-linked myotubular myopathy is associated with epigenetic alterations and is ameliorated by HDAC inhibition. Acta Neuropathol.

Clinical and Translational Medicine

Impact Factor: 11.5

Zhang Y et al. (Aug 2021). Oncogenic PAX6 elicits CDK4/6 inhibitor resistance by epigenetically inactivating LATS2-Hippo signaling. Clin Transl Med.

Cell Communication and Signaling

Impact Factor: 9.4

Xu X et al. (Mar 2023). Induction of DNMT1-dependent demethylation of SHP-1 by the natural flavonoid compound Baicalein overcame Imatinib-resistance in CML CD34(+) cells. Cell Commun Signal.

Get ready for fast, sensitive DNMT activity quantification

View Product Details

Frequently asked questions

How long does the protocol take?

The complete workflow is approximately 2 hours and 10 minutes.

What is the assay’s sensitivity?

It can detect as little as 0.2 ng of purified DNMT enzyme.

Which sample types are supported?

The kit measures DNMT activity/inhibition from nuclear extracts and purified enzymes.

How is activity detected?

By direct antibody capture of DNMT-induced methylation on a DNA substrate, providing high specificity versus indirect MBD-based methods.

Is the format automation-friendly?

Yes. The kit uses a 96 strip-well microplate for flexible layouts and high-throughput workflows.

Also Available: DNA Methylation Antibodies

Discover rigorously validated antibodies optimized for detecting DNA methylation with precision and reliability across diverse research applications.

Explore DNA Methylation Antibodies
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