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EpiQuik Methyl-Histone H3K4 ChIP Kit

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For immunoprecipitating H3K4me2 chromatin from cell input samples via microplate format

Citations (3) | Write a Review
Suggested Workflow
Chromatin Isolation
 
 
Chromatin Shearing
 
 
Methyl-Histone ChIP
 
 
PCR Analysis
 
Schematic procedure of the EpiQuik™ Methyl-Histone H3K4 ChIP Kit.
Analyses of histone H3K4me2 levels in the promoter of DKK3 gene by ChIP (using the EpiQuik™ Methyl-Histone H3K4 ChIP Kit) and subsequent qPCR: the experiments were performed on D458Med cell line comparing the fold enrichment over background after 24 hours of TSA treatment with respect to untreated cells.
Input Type: Chromatin
Research Area: Chromatin & Transcription
Target Application: Immunoprecipitation
Vessel Format: 96-Well Plate
100% Guarantee: 6 months
Catalog No.SizePriceQty
P-2007-2424 reactions $318.00 
P-2007-4848 reactions $524.00 
Availability: Usually Ships In 1-2 Days 
Product Overview

The EpiQuik™ Methyl-Histone H3K4 ChIP Kit is a convenient package of tools that allows the experimenter to investigate interactions of histone H3K4 methylation and DNA in vivo efficiently. The entire procedure can be completed within 5 hours and produces far more superior results than any competitor kits. The EpiQuik™ Methyl-Histone H3K4 ChIP Kit is suitable for combining the specificity of immunoprecipitation with qualitative and quantitative PCR, ChIP-Seq, and ChIP-on-chip. This kit has the following advantages:

  • The fastest procedure available, which can be finished within 5 hours.
  • Strip microwell format makes the assay flexible: manual or high throughput.
  • Columns for DNA purification are included: save time and reduce labor.
  • Compatible with all DNA amplification-based approaches.
  • Simple, reliable, and consistent assay conditions.

Background Information
Methylation on histone H3 at lysine 4 (H3K4) plays an important role in epigenetic regulation of the genes, it was well demonstrated that methylation on H3K4 leads to chromatin gene activation in many organisms. Dimethylation at H3K4 may serve as a global epigenetic mark in euchromatin. Thus, identification of genes activated through H3K4 methylation is of particular significance for understanding and analyzing biological processes under the normal and pathological conditions. Chromatin Immunoprecipitation (ChIP) is a powerful technique for studying protein-DNA interaction in vivo. ChIP also offers an advantageous tool that allows identification of activated genes associated with methylated histone H3K4. ChIP coupled with microarrays could be further used for profiling or mapping histone H3K4 methylation patterns.

Principle & Procedure
This ChIP Kit inlcudes all reagents required for carrying out a successful chromatin immunoprecipitation for methyl-histone H3K4 from mammalian cells. Particularly, this kit includes a ChIP-grade di-methyl histone H3K4 antibody and a negative control normal mouse IgG. Chromatin from the cells is extracted, sheared, and added into the microwell immobilized with the antibody. DNA is released from the antibody-captured methyl-histone H3K4 protein-DNA complex, reversed, and purified through the specifically designed F-Spin Column. Eluted DNA can be used for various down-stream applications.

Starting Materials
Starting materials can include various cell samples. In general, the input amount should be from 1,000,000 to 4,000,000 cells for each reaction.

Product Components

CP1 (Wash Buffer)
CP2 (Antibody Buffer)
CP3A (Pre-Lysis Buffer)
CP3B (Lysis Buffer)
CP4 (ChIP Dilution Buffer)
CP5 (DNA Release Buffer)
CP6 (Reverse Buffer)
CP7 (Binding Buffer)
CP8 (Elution Buffer)
Protease Inhibitor Cocktail (100X)*
Proteinase K (10 mg/ml)*
Normal Mouse IgG (0.5 mg/ml)*
Anti-Dimethyl-H3-K4 (1 mg/ml)*
8-Well Assay Strips (with Frame)
8-Well Strip Caps
F-Spin Column
F-Collection Tube
User Guide

* For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap.

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Material Safety Data Sheet]
Product Citations

Chen J et. al. (June 2016). Aberrant Epigenetic Alterations of Glutathione-S-Transferase P1 in Age-Related Nuclear Cataract. Curr Eye Res. :1-9.

Kraus D et. al. (April 2014). Nicotinamide N-methyltransferase knockdown protects against diet-induced obesity. Nature. 508(7495):258-62.

Valdora F et. al. (April 2013). Epigenetic Silencing of DKK3 in Medulloblastoma. Int J Mol Sci. 14(4):7492-505.

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