Save $100 on Antibodies Save $100 when you buy $500 in antibodies. Use code E1705ABR. Expires June 30th. » Learn More
Epigentek Home
 
Epigentek Free Shipping

EpiQuik Tissue Methyl-Histone H3K9 ChIP Kit

Share:

For immunoprecipitating H3K9me2 chromatin from tissue input samples via microplate format

Citations (4) | Write a Review
Suggested Workflow
Chromatin Isolation
 
 
Chromatin Shearing
 
 
Methyl-Histone ChIP
 
 
PCR Analysis
 
Schematic procedure of the EpiQuik™ Tissue Methyl-Histone H3K9 ChIP Kit.
ChIP analysis of H3K9me2 in Syce1 gene promoters using the EpiQuik™ Tissue Methyl-Histone H3K9 ChIP Kit. Precipitated DNA from wild-type and 15-2KO lungs were examined by quantitative PCR. Error bars represent the standard deviation of three experiments.
Input Type: Chromatin
Research Area: Chromatin & Transcription
Target Application: Immunoprecipitation
Vessel Format: 96-Well Plate
100% Guarantee: 6 months
Catalog No.SizePriceQty
P-2008-2424 reactions $338.00 
P-2008-4848 reactions $539.00 
Availability: Usually Ships In 1-2 Days 
Product Overview

The EpiQuik™ Tissue Methyl-Histone H3K9 ChIP Kit is a convenient package of tools that allows the experimenter to investigate interactions of histone H3K9 methylation and DNA in a variety of mammalian tissues. The entire procedure can be completed within 5 hours and produces far more superior results than any competitor kits. The EpiQuik™ Tissue Methyl-Histone H3K9 ChIP Kit is suitable for combining the specificity of immunoprecipitation with qualitative and quantitative PCR, ChIP-Seq, and ChIP-on-chip. This kit has the following advantages: 

  • The fastest procedure available, which can be finished within 5 hours.
  • Strip microwell format makes the assay flexible: manual or high throughput.
  • Columns for DNA purification are included: save time and reduce labor.
  • Compatible with all DNA amplification-based approaches.
  • Simple, reliable, and consistent assay conditions.

Background
The position of the histone H3 at lysine 9 (H3K9) is important for epigenetic regulation of the genes, as it can be either methylated or acetylated. It was well demonstrated that switching acetylation to methylation on H3K9 leads to chromatin gene silencing in many organisms. Methylated H3K9 has been involved in tumorigenesis through inactivation of tumor suppression genes. Thus, identification of genes silenced through H3K9 methylation is of particular significance for understanding and analyzing biological processes under the normal and pathological conditions. Chromatin Immunoprecipitation (ChIP) is a powerful technique for studying protein-DNA interaction in vivo. ChIP also offers an advantageous tool that allows identification of silenced genes associated with methylated histone H3K9. ChIP coupled with microarrays could be further used for profiling or mapping histone H3K9 methylation patterns. Requirement for using ChIP to analyze histone-DNA interaction in solid tissues and tumors is rapidly increasing.

Principle & Procedure
This ChIP Kit contains all reagents necessary for carrying out a successful chromatin immunoprecipitation for methyl-histone H3K9 from mammalian tissues. Particularly, this kit includes a ChIP-grade di-methyl histone H3K9 antibody and a negative control normal mouse IgG. Chromatin from the cells is extracted, sheared, and added into the microwell immobilized with the antibody. DNA is released from the antibody-captured methyl-histone H3K9 protein-DNA complex, reversed, and purified through the specifically designed F-Spin Column. Eluted DNA can be used for various down-stream applications.

Starting Materials
Starting materials can include various tissue samples. In general, the input amount should be 20 mg of tissue for each reaction.

Product Components

CP1 (Wash Buffer)
CP2 (Antibody Buffer)
CP3 (Lysis Buffer)
CP4 (ChIP Dilution Buffer)
CP5 (DNA Release Buffer)
CP6 (Reverse Buffer)
CP7 (Binding Buffer)
CP8 (Elution Buffer)
Homogenizing Buffer
Protease Inhibitor Cocktail (100X)*
Proteinase K (10 mg/ml)*
Normal Mouse IgG (0.5 mg/ml)*
Anti-Dimethyl-H3-K9 (1 mg/ml)*
8-Well Assay Strips (with Frame)
8-Well Strip Caps
F-Spin Column
F-Collection Tube
User Guide

* For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap.

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Material Safety Data Sheet]
Epigentek Rewards
Related Products

Researchers Also Purchased
EpiQuik Tissue Methyl-Histone H3K4 ChIP Kit
EpiQuik Tissue Methyl-Histone H3K4 ChIP Kit
EpiQuik Tissue Acetyl-Histone H3 ChIP Kit
EpiQuik Tissue Acetyl-Histone H3 ChIP Kit
EpiQuik Global Acetyl Histone H3K9 Quantification Kit (Colorimetric)
EpiQuik Global Acetyl Histone H3K9 Quantification Kit (Colorimetric)
EpiQuik Tissue Acetyl-Histone H4 ChIP Kit
EpiQuik Tissue Acetyl-Histone H4 ChIP Kit
Epigentek is an epigenetics company that specializes in epigenetic kits, antibodies, reagents, and services for epigenetic research in DNA methylation, histone modification, and chromatin studies. Terms & Conditions | Privacy Policy | Site Map
Copyright © 2017 Epigentek Group Inc. All rights reserved.