Histone Acetylation

EpiQuik™ histone acetylation assays are ELISA-based methods for quantifying global levels of the most important and well-characterized acetylated H3/H4 marks from a variety of sample types, including cells (in situ), plasma, serum, and histone extracts. The EpiQuik™ technology allows for rapid and efficient colorimetric detection of acetylation modifications, conveniently eliminating the need for radioactivity, electrophor...

Histone Acetyltransferases (HATs) and Their Role in Gene Expression Histone acetyltransferases (HATs) are enzymes that transfer acetyl groups from acetyl-coenzyme A to the lysine residues of histones. These enzymes play important roles in regulating gene expression by modifying the structure of chromatin, making it more accessible for transcription. HATs and Transcription HATs directly correspond to transcription by selectively acetylating the epsilon-amino grou...

Epigenase™ histone deacetylase assays are ELISA-based methods for measuring the total activity/inhibition of HDACs or SIRTs from both purified enzyme and nuclear extract samples. The Epigenase™ technology allows for direct detection of enzyme-converted deacetylated products rather than trypsin-based peptide cleavage, thus eliminating assay interference caused by DMSO and thiol-containing chemicals, trypsin, and cellular ly...